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Resource library of rat with gene mutation and preparation method thereof

A resource bank and genome technology, applied in the biological field, can solve the problem of lack of genetic mutation rat resource bank and so on

Pending Publication Date: 2017-03-22
浙江耶大生物医药有限公司
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  • Abstract
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Problems solved by technology

[0007] In order to solve the problem of the lack of gene mutation rat resource library that can be used for gene function research and new drug development, the present invention provides a genetic mutation rat strain resource obtained by introducing insertion mutations into experimental rats using the piggyBac transposon system library

Method used

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  • Resource library of rat with gene mutation and preparation method thereof
  • Resource library of rat with gene mutation and preparation method thereof
  • Resource library of rat with gene mutation and preparation method thereof

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Embodiment Construction

[0037] The present invention provides a gene mutant rat strain resource library obtained by introducing insertion mutation into experimental rats by using the piggyBac transposon system, which has the advantage that it can be used for animal phenotype analysis, gene function research, human genetic disease simulation, and new drugs Screening and discovery of new drug targets.

[0038] Below in conjunction with specific examples, the key steps of the preparation and application of the present invention are further illustrated.

[0039] 1. Gene trap construction.

[0040] The present invention utilizes a gene trapping construct designed according to the principles of RNA transcription and splicing. More specifically, the piggyBac transposon-based gene trap construction includes two functional parts: one is the promoter trap construction, that is, the upstream gene transcription termination cassette, which contains the RNA splicing acceptor (SA) and polyA termination signal; the...

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Abstract

The invention belongs to the field of biotechnology, and relates to a resource library of rat strains with gene mutation and a preparation method thereof. The rats with gene mutation in the resource library can be obtained from rat spermatogonial stem cells, embryonic stem cells or fertilized ovum with piggyBac transposon insertion mutation. The rats with gene mutation in the resource library also can be obtained from shifting the transposon in germ cells of male rats with mutation. The library can be applied to animal phenotype analysis, gene function research, human disease simulation, medicament screening and drug target development.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a gene mutant rat strain resource bank and a preparation method thereof. The invention can be used for animal phenotype analysis, gene function research, human disease simulation, drug screening and drug target development. Background technique [0002] Gene mutations can change the DNA nucleotide sequence of a specific gene, and specific types include base substitution mutations, frameshift mutations, deletion mutations, and insertion mutations. Gene mutations cause changes in the expression or function of specific gene products, resulting in changes in biological processes at the cellular level and abnormal phenotypes at the individual animal level (Introduction to Genetic Analysis, 10th edition, edited by Griffiths et al. ). [0003] Experimental animal models carrying genetic mutations are very important tools in biological research, new drug development and livestock breed impro...

Claims

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Application Information

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IPC IPC(8): C40B40/06C40B50/06C12Q1/68
Inventor 潘雨堃
Owner 浙江耶大生物医药有限公司
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