Resource library of rat with gene mutation and preparation method thereof

Abstract

The invention belongs to the field of biotechnology, and relates to a resource library of rat strains with gene mutation and a preparation method thereof. The rats with gene mutation in the resource library can be obtained from rat spermatogonial stem cells, embryonic stem cells or fertilized ovum with piggyBac transposon insertion mutation. The rats with gene mutation in the resource library also can be obtained from shifting the transposon in germ cells of male rats with mutation. The library can be applied to animal phenotype analysis, gene function research, human disease simulation, medicament screening and drug target development.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a gene mutant rat strain resource bank and a preparation method thereof. The invention can be used for animal phenotype analysis, gene function research, human disease simulation, drug screening and drug target development. Background technique [0002] Gene mutations can change the DNA nucleotide sequence of a specific gene, and specific types include base substitution mutations, frameshift mutations, deletion mutations, and insertion mutations. Gene mutations cause changes in the expression or function of specific gene products, resulting in changes in biological processes at the cellular level and abnormal phenotypes at the individual animal level (Introduction to Genetic Analysis, 10th edition, edited by Griffiths et al. ). [0003] Experimental animal models carrying genetic mutations are very important tools in biological research, new drug development and livestock breed impro...

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Problems solved by technology

[0007] In order to solve the problem of the lack of gene mutation rat resource library that can be used for gene function research and new drug development, the present

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