Building method for liver cancer rat model

A technology of rat model and establishment method, which is applied in the fields of gene transcriptomics and proteomics, and can solve problems such as the inability to completely and correctly predict various modifications of the start and end positions of splicing and splicing

Inactive Publication Date: 2018-04-06
南宁科城汇信息科技有限公司
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Problems solved by technology

However, with the completion of the Human Genome Project, it was found that only from the perspective of genomics can not completely correctly predict

Method used

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Embodiment

[0019] The establishment method of this liver cancer rat model consists of the following steps:

[0020] ① Animals: 40 clean-grade Wistar male rats, weighing 150 ± 20g; randomly divided into 30 test groups and 10 control groups; kept in a semi-barrier system according to the feeding requirements of clean-grade animals;

[0021] ② Model making: Diethylnitrosamine solution with a concentration of 100 μg Pml was prepared with sterilized tap water (preserved in the dark, freshly prepared), and the rats in the test group were free to drink, replaced once a day, and changed to normal after 3 months. Sterilized tap water, continued to observe for 2 months, the whole experiment process lasted for 5 months; the control group drank sterilized tap water throughout the experiment process;

[0022] ③ Liver cancer determination: Animals were killed by excessive injection of pentobarbital sodium, the abdominal cavity was opened, liver tissue was taken and fixed with 10% formaldehyde, and aft...

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Abstract

The invention discloses a building method for a liver cancer rat model. The method comprises the steps that 1, animals are included, wherein the clean class is Wistar, the number of male rats is 40, the weight is 150+/-20 g, and the male rats are randomly divided into an experimental group including 30 rats and a control group including 10 rats; the animals are reared in a semi-barrier system according to the raising requirement of the clean class of the animals; 2, modeling is performed, wherein sterile tap water is used for preparing a diethylnitrosamine solution with the concentration being100 microgramPml (the solution is kept in a dark place and freshly prepared), the solution is freely drunk by the rats in the experimental group, the solution is changed one time every day, after three months, the solution is replaced with ordinary sterilization tap water, observation continues to be performed for 2 months, and the whole experimental process lasts 5 months; according to the control group, the rats drink the sterilization tap water in the whole experiment process; 3, liver cancer is determined; 4, liver tissue is sampled. A clinic sample is adopted for performing positioning and quantified expression verification, the evidence related clinically is found, the clinic value is evaluated, and the novel clue is provided for liver cancer pathogenesis and liver cancer mechanismresearch. The liver cancer key molecule screened by a question for discussion is adopted for researching a liver cancer marker related to early discovery, classification and evaluation prognosis, andthe research basis is laid for selecting a more effective and accurate liver cancer treatment target position.

Description

technical field [0001] The invention relates to the fields of gene transcriptomics and proteomics, in particular to a method for establishing a liver cancer rat model. Background technique [0002] One of the most important fields in biological and medical research is the observation of the structure, function and regulation of biological systems and life processes. However, in the past few hundred years, biologists have been focusing on the expression changes and functions of individual genes or proteins in biological systems, but cannot study the changes in living systems from a global and holistic perspective. With the advancement of medicine, people have found that many diseases, especially cancer, are often caused by the synergistic effects of multiple factors, multiple genes, and multiple pathways. This requires a technology and means that can comprehensively, dynamically and systematically study the life system, so the concept of "omics" came into being [3] . Howev...

Claims

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Application Information

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IPC IPC(8): A61K31/655
CPCA61K31/655
Inventor 于思创王海云
Owner 南宁科城汇信息科技有限公司
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