Animal model construction method for study of spleen deficiency and dampness syndrome and application thereof

A technology of spleen deficiency and dampness, animal model, applied in the field of biomedicine, to achieve the effects of simple modeling method, good practicability, easy operation and promotion

Active Publication Date: 2019-12-13
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is: in view of the defects and deficiencies in the prior art that there is no quantitative evaluation standard for the syndrome characterization of the animal model of spleen deficiency and the model is simple and conforms to the development process model of spleen deficiency and dampness in humans, it provides a Animal model construction method and its application in the study of spleen deficiency and dampness syndrome

Method used

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  • Animal model construction method for study of spleen deficiency and dampness syndrome and application thereof
  • Animal model construction method for study of spleen deficiency and dampness syndrome and application thereof
  • Animal model construction method for study of spleen deficiency and dampness syndrome and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Construction of a rat model in stagnation and damp-heat syndrome stages

[0030] (1) Animal grouping and feeding: 100 SPF grade male SD rats, weighing 160±20g, provided by the Experimental Animal Science and Technology Center of Jiangxi University of Traditional Chinese Medicine [SCXK (Gan) 2018-0003]. Animal feeding conditions: natural lighting according to circadian rhythm, rearing in SPF level experimental animal barrier system, laboratory temperature 23±2°C, relative humidity 53±3%. After a week of adaptive feeding, the animals were randomly divided into a model group (90 rats) and a normal group (10 rats), and they were fed with a high-fat diet (60% fat content) and a normal basic maintenance diet (10% fat content, respectively). quantity).

[0031] (2) Feed:

[0032] The normal basic maintenance feed ingredients are: choline chloride 0.2%, corn 30%, wheat 25.5%, honey yeast 2.5%, calcium hydrogen phosphate 2%, stone powder 1%, growth premix 1%, salt 0.3...

Embodiment 2

[0041] Example 2: Construction of Rat Models in Stages of Spleen Deficiency and Stasis Syndrome

[0042] (1) Animal grouping and feeding: the normal group (10 rats) in Example 1 and the rats in the model group (73 rats) conforming to stagnation and damp-heat syndrome continued to be fed in the SPF-level experimental animal barrier system in Example 1, At this stage, the two groups of animals were placed in 2 independent experimental rooms for feeding. The rats in the normal group (10) were fed under the same conditions as in Example 1, and continued to feed the normal basic maintenance feed (10% fat content), while the model Rats in the group (73 rats) were fed with a high-fat diet (60% fat content) by raising the relative humidity to 90±3%.

[0043] (2) Detection of indicators: Same as Example 1.

[0044] (3) Rats with spleen deficiency and blood stasis syndrome: Combining some clinical characteristics of rats with the diagnostic criteria of human spleen deficiency, it is de...

Embodiment 3

[0055] Example 3: Application of Rat Model of Spleen Deficiency and Excessive Dampness Syndrome

[0056] The first is to combine some clinical characteristics of spleen-deficiency rats with the diagnostic criteria of human spleen-deficiency to determine that the rats have abdominal obesity, loss of appetite, loose stool, dark yellow urine, purple tongue or watery coating. , fatigue, fatigue, drowsiness, hair loss, squinting and other symptoms and signs to assist in the diagnosis of depression, damp heat, spleen deficiency and blood stasis syndrome in humans. Further, in the study on the syndrome of spleen deficiency and dampness, the characteristics of intestinal bacteria Akkermansia ( AKK ) abundance and the ratio of kynurenine to tryptophan increased to assist in the diagnosis of human spleen deficiency and blood stasis syndrome. Further, in the study of spleen deficiency and dampness syndrome, after intervening the model with simvastatin and rosiglitazone in the positive...

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Abstract

The invention relates to the technical field of biological medicine, and specifically discloses an animal model construction method for the study of spleen deficiency and dampness syndrome. The animalmodel construction method comprises two steps that 1, a male rat is selected and fed with high-fat feed, the relative humidity is 53+ / -3%, a dark and quiet environment is maintained, the deep color and light color of the back face of the tongue of the rat are regularly subjected to recording, tongue picture capture, comparative analysis and quantitative grading, physiological and biochemical indexes such as intestinal florae, tryptophan and metabolites of the intestinal florae and tryptophan are measured regularly, physical signs such as hair color, urine and excrement and mental conditions of the rat are recorded regularly, and the construction of the rat model in the depression and damp-heat syndrome stage is completed until 4-6 weeks; 2, the rat in the depression and damp-heat syndromestage continues to be fed with the high-fat feed, the relative humidity is increased to 90+ / -3%, the indexes continue to be detected and observed until 8-10 weeks, and the construction of the rat model in the spleen deficiency and stasis syndrome stage is completed. The rat model construction has the advantages that the model construction method is simple, easy to operate and promote, and close to clinic.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for constructing an animal model for research on syndromes of spleen deficiency and dampness and its application. Background technique [0002] Since the diet type in the "enrichment era" is mainly "fat, sweet and thick", the intake of fat, sweet and thick food is too much, and there are more and more people with spleen deficiency and dampness syndrome. Spleen deficiency and excessive dampness will not only cause anorexia, diarrhea, anal burning pain, fatigue and other symptoms, but also because the spleen is the acquired foundation, the source of qi and blood biochemistry, and is located in the middle burner. Therefore, spleen deficiency and dampness are also related to many viscera diseases such as liver and stomach. Due to the importance of the spleen, the development of medicines for invigorating the spleen and removing dampness is becoming more and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A23K10/18A23K10/22A23K10/30A23K10/37A23K20/105A23K20/147A23K20/158A23K20/20A23K20/26A23K50/50
CPCA01K67/02A23K10/18A23K10/22A23K10/30A23K10/37A23K20/105A23K20/147A23K20/158A23K20/20A23K20/26A23K50/50Y02P60/87
Inventor 曾治君吉燕华张雅莉姜璎娜张吉日木吐徐国良刘红宁严小军
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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