Young citrus fruit health-care food for weight management and preparation method of young citrus fruit health-care food

Abstract

The invention provides a young citrus fruit health-care food for weight management and a preparation method thereof, the young citrus fruit health-care food for weight management comprises the following components in parts by weight: 15 parts of young citrus fruit powder, 10 parts of excipient and 10 parts of first flavoring agent; 30 to 50 parts of an excipient; the content of the first flavoring agent is 16.5 to 27.5 parts. According to the method disclosed by the invention, the two components of citrus polyphenol and synephrine in the young citrus fruits can be reserved to the greatest extent. Citrus polyphenol contained in the citrus functional chewable tablet can inhibit fat digestion activity and inhibit absorption of fat food by a human body, synephrine can cause vasoconstriction, blood pressure rise and bronchial muscle relaxation, stimulate lipolysis and improve the metabolism rate and oxidation degree of fat by increasing the heat generation effect, and the fat digestion activity can be inhibited. Under the condition of normal diet, energy intake and fat decomposition are effectively reduced, so that the effect of reducing body weight can be achieved.

Description

Technical Field

[0001] The invention relates to the field of health food, in particular to a citrus young fruit health food for weight management and a preparation method thereof. Background Art

[0002] In recent years, with the improvement of living standards, the increase of work pressure and irregular diet, dyslipidemia and obesity have occurred. The "Report on the Nutrition and Chronic Disease Status of Chinese Residents (2020)" shows that the situation of overweight and obesity among Chinese residents is severe. The overweight and obesity rates of adult residents are 34.3% and 16.4% respectively; the overweight and obesity rates of children and adolescents aged 6 to 17 are 11.1% and 7.9% respectively; the overweight and obesity rates of children under 6 are 6.8% and 3.6% respectively. Obesity is a chronic metabolic disease in which energy intake exceeds energy consumption, resulting in excessive accumulation of fat in the body, which is harmful to human health.

[0003] With the improvement of health awareness, more and more people realize that obesity has become a risk factor that seriously threatens people's physical and mental health and cannot be ignored. The weight loss products on the market now mainly include weight loss pills and weight loss health foods. Although the weight loss effect of weight loss pills is obvious, they also have disadvantages such as large side effects and strong drug dependence. Although weight loss health foods do not have side effects and dependence, the weight loss effect of most weight loss health foods is not obvious.

[0004] Citrus fruits are rich in nutrients. They not only contain a large amount of pectin, oligosaccharides, organic acids, various amino acids and other nutrients, but also contain a variety of physiologically active ingredients that promote human health and prevent and treat some diseases, such as limonoids, alkaloids, volatile oils, phenolic substances, etc. During the growth and development of citrus fruits, a large number of natural fruit drops and young fruits produced by artificial fruits and vegetables will be produced. Compared with mature citrus fruits, the content of polyphenols, flavonoids, synephrine and limonoids in physiological fruit drops and thinning fruits of citrus is higher. Studies have shown that active ingredients such as synephrine and polyphenols in citrus have the effects of lowering lipids and losing weight. At present, the application of citrus flavonoid products in weight management is mainly as an auxiliary ingredient, which is compatible with other Chinese medicinal materials to prepare products with weight loss effects. In addition, due to the imperfect preparation process, polyphenols and synephrines are often lost in large quantities during the preparation of young citrus fruits. Therefore, the application of citrus flavonoid products in weight management needs further development. Summary of the invention

[0005] The purpose of the present invention is to provide a citrus young fruit health food for weight management and a preparation method thereof. The method uses young fruits produced by naturally fallen fruits and artificial fruits and vegetables as raw materials, which not only has a low production cost, but also has a good effect of managing weight.

[0006] In order to solve the problems existing in the prior art, the technical solution adopted by the present invention is: In a first aspect, the present invention provides a citrus young fruit health food for weight management, comprising citrus young fruit powder, an excipient and a first flavoring agent. In terms of weight, the citrus young fruit powder is 15 parts; the excipient is 30-50 parts; and the first flavoring agent is 16.5-27.5 parts.

[0007] Furthermore, the excipient is microcrystalline cellulose and / or mannitol; and the first flavoring agent is oligofructose and citric acid.

[0008] Furthermore, the excipient is composed of the following raw materials in parts by weight: 10-20 parts of microcrystalline cellulose and 20-30 parts of mannitol.

[0009] Furthermore, the first flavoring agent is composed of the following raw materials in parts by weight: 1.5-2.5 parts of citric acid, 15-25 parts of oligofructose.

[0010] Preferably, the following raw materials are included in parts by weight: 15 parts of citrus young fruit powder, 15 parts of microcrystalline cellulose, 30 parts of mannitol, 15 parts of oligofructose, and 1.5 parts of citric acid.

[0011] Furthermore, it also includes a binder, which is ethanol with a volume fraction of 75%.

[0012] Furthermore, it also includes a lubricant, which is magnesium stearate, and the lubricant is 4 parts by weight.

[0013] Furthermore, it also includes a second flavoring agent, which is citrus flavor, and the citrus flavor is 2 parts by weight.

[0014] Furthermore, the health food is a chewable tablet.

[0015] In a second aspect, the present invention provides a method for preparing the citrus young fruit health food for weight management as described in the first aspect, comprising the following steps: ① Preparation of citrus young fruit powder: Wash the citrus young fruit that meets the requirements in clean water, drain the water, slice it, freeze-dry it for 48 hours, crush it through a 100-mesh sieve, put it in a sealed container and store it in a -20℃ refrigerator for later use; ② Weigh the citrus young fruit powder, excipients, and the first flavoring agent according to the proportion, mix them evenly, and obtain a mixture; ③ The mixture prepared in step ② is added with a binder to make a soft material, granulate, and dry to obtain dry granules; ④ After the dry granules prepared in step ③ are uniformly mixed with the second flavor corrective and the lubricant, tableting is performed.

[0016] The advantages and beneficial effects of the present invention are: The citrus young fruit health food for weight management provided by the present invention is prepared by compounding citrus young fruit powder with specific fillers, flavoring agents, and lubricants in a specific ratio and a specific method, so that the two components of citrus polyphenols and synephrine in the citrus young fruit can be retained to the greatest extent. The chewable tablet of the present invention can inhibit fat digestion activity, inhibit the absorption of fatty foods by the human body, cause vasoconstriction, increase blood pressure and relax bronchial muscles, stimulate fat decomposition, and increase the metabolic rate and oxidation degree of fat by increasing thermogenesis, effectively reduce energy intake and fat decomposition under normal diet conditions, thereby achieving the effect of weight loss.

[0017] The present invention uses young citrus fruits as raw materials, which can not only control body weight, reduce body fat, lose weight, promote calorie consumption, and provide a variety of product options for obese and hyperlipidemia people, but also reasonably utilize citrus fallen fruits and vegetables and fruits, turning waste into treasure, and increasing the added value of citrus. At the same time, it has practical significance for the development of the citrus industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 The present invention is a flow chart of the preparation method of the citrus young fruit health food for weight management. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present application to clearly describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments in the present application belong to the scope of protection of this application.

[0020] The experimental methods in the following examples, unless otherwise specified, are all conventional methods, and are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. The materials, reagents, etc. used in the following examples, unless otherwise specified, can all be obtained from commercial channels.

[0021] The weight parts involved in the present invention can be μg, mg, g, kg and other units of measurement known in the food field. Example 1

[0022] This embodiment provides a citrus young fruit health food for weight management, which includes the following raw materials in parts by weight: 15 parts of citrus young fruit powder, 15 parts of microcrystalline cellulose, 30 parts of mannitol, 15 parts of oligofructose, and 1.5 parts of citric acid.

[0023] like Figure 1 As shown, the present embodiment provides a method for preparing a citrus health food for weight management, comprising the following steps: ①Put the selected citrus young fruits that are free of disease, insect pests and corruption in clean water for washing, draining, slicing and other pretreatments. Then freeze-dry them for 48 hours, crush them through a 100-mesh sieve, put them in a sealed container and store them in a -20℃ refrigerator for later use.

[0024] ② Mix the citrus young fruit powder, microcrystalline cellulose, mannitol, oligofructose and citric acid, and stir them evenly to prepare the health food of the present application.

[0025] In order to facilitate carrying and eating, we can further prepare it into granules: ③ Use an appropriate amount of food-grade ethanol with a volume fraction of 75% as a binder to make the soft material. The amount of the binder is based on the hardness of the soft material being "a ball can be formed by hand, and it will fall apart when lightly pressed". The above soft material is squeezed through a 20-mesh screen to form granules, and the granules are moved into a drying oven and dried at 60°C for about 35 minutes. The moisture content of the granules is controlled at 5%, and granulation is performed.

[0026] Furthermore, it can also be prepared into sheets: ④ Add 4 parts by weight of magnesium stearate and 2 parts by weight of citrus flavor to the granules prepared in step ③, mix well, compress into tablets, and package them at 0.27 g / tablet. The tablet weight difference should be within 5%.

[0027] The active ingredients of the chewable tablets prepared in this example were detected by the following method: 1. Total phenol content Take a chewable tablet sample prepared in Example 1 and mix it in a methanol solution (50% (v / v)) at a ratio of 1:20 (w / v) to obtain a chewable tablet diluent. The sample is stirred at room temperature for 3 hours and then filtered. The filtrate is retained for the determination of total phenols, total flavonoids, and synephrine content.

[0028] The total phenol content in the chewable tablet samples was determined by the FoLin-Ciocalteu colorimetric method, and gallic acid was used as the standard. Preparation of the standard curve: 0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6 and 0.7 mL of gallic acid standard solution (0.1 mg / mL) were taken in a 10 mL volumetric flask, deionized water was added to 1 mL, and 0.5 mL of Folin phenol reagent was added after shaking. After standing for 1 min, 1.5 mL of 20% sodium carbonate solution (w / v) was added and then made up to volume with deionized water. After shaking, it was kept away from light at room temperature for 1 h, and the absorbance was measured at 760 nm to draw the standard curve.

[0029] Determination of samples: Accurately weigh 1 mL of the chewable tablet dilution and determine the absorbance at 760 nm according to the above method. Perform the determination three times in parallel. Substitute the results into the standard curve to obtain the total phenol concentration of the chewable tablet sample dilution.

[0030] 2. Total flavonoid content The total flavonoids were determined by aluminum chloride colorimetry, and the standard was rutin. Preparation of the standard curve: Accurately pipette 0, 1, 2, 3, 4, and 5 mL of rutin standard solution (0.1 mg / mL) into 10 mL colorimetric tubes, add 0.3 mL of 5% sodium nitrite (v / w) and mix well. After reacting for 5 min, add 0.3 mL of 10% aluminum chloride (w / v) to the test tube and react for 6 min. Then, add 4 mL of 1 mol / L sodium hydroxide and mix well, and add deionized water to a final volume of 10 mL. The mixture reacts at room temperature for 12 min, and the absorbance of the solution is measured at 510 nm to draw a standard curve.

[0031] Determination of samples: Accurately weigh 1 mL of the chewable tablet dilution and determine the absorbance at 510 nm according to the above method. Perform the determination three times in parallel. Substitute the results into the standard curve to obtain the flavonoid concentration of the chewable tablet dilution.

[0032] 3. Synephrine content Refer to the total phenol extraction method and use the extract for the determination of synephrine. The method uses liquid chromatography: Take an appropriate amount of chewable tablet sample and mix it in methanol solution (50% (v / v)) at a ratio of 1:20 (w / v) to obtain a chewable tablet diluent. Stir the sample at room temperature for 3 hours, centrifuge and aspirate the supernatant, filter it through a 0.22 μm microporous filter membrane, and place it in a brown injection vial. Keep the filtrate for the determination of synephrine content.

[0033] Chromatographic conditions for synephrine determination: Chromatographic column: InertsilODS-3 chromatographic column (4.6mmx250mm, 5μm); mobile phase A: water (containing 0.06% phosphoric acid, 0.1% sodium dodecyl sulfate), B: methanol; isocratic elution, A:B-30%:70% (V / V), flow rate 0.8mL / min, column temperature 40℃; injection volume: 5μL; UV detection wavelength is 225nm; chromatographic workstation, Breeze system of Waters Company, USA.

[0034] Preparation of Synephrine Standard Solution: Accurately weigh 15.20 mg of synephrine standard into a 25 ml brown volumetric flask, dissolve and dilute with chromatographic grade methanol to obtain a stock solution of synephrine standard, accurately pipette the stock solution, and dilute it gradiently with chromatographic grade methanol to a synephrine concentration of 38 μg / mL-486 μg / mL. Seal and store at 4°C for later use.

[0035] Sample determination: Substitute the sample data results into the standard curve to obtain the synephrine concentration of the chewable tablet dilution.

[0036] After testing, each chewable tablet prepared in this example contained 2.420 mg of total phenols, 2.140 mg of total flavonoids, and 5.180 mg of synephrine. Example 2

[0037] This embodiment provides a citrus young fruit health food chewable tablet for weight management, which is composed of the following raw materials in parts by weight: 15 parts of citrus young fruit powder, 15 parts of microcrystalline cellulose, 30 parts of mannitol, 25 parts of oligofructose, 1.5 parts of citric acid, 2 parts of citrus flavor, 4 parts of magnesium stearate, and an appropriate amount of 75% by volume ethanol.

[0038] The preparation method of this embodiment is the same as that of embodiment 1.

[0039] The active ingredients of the chewable tablets prepared in this example were detected according to the method described in Example 1. The chewable tablets prepared in this example each contained 2.158 mg of total phenols, 1.909 mg of total flavonoids, and 4.620 mg of synephrine. Example 3

[0040] This embodiment provides a citrus health food chewable tablet for weight management, which is composed of the following raw materials in parts by weight: 15 parts of citrus young fruit powder, 15 parts of microcrystalline cellulose, 30 parts of mannitol, 20 parts of oligofructose, 1.5 parts of citric acid, 2 parts of citrus flavor, 4 parts of magnesium stearate, and an appropriate amount of 75% ethanol by volume.

[0041] The preparation method of this embodiment is the same as that of embodiment 1.

[0042] The active ingredients of the chewable tablets prepared in this example were detected according to the method described in Example 1. The chewable tablets prepared in this example each contained 2.281 mg of total phenols, 2.018 mg of total flavonoids, and 4.884 mg of synephrine. Example 4

[0043] This embodiment provides a citrus young fruit health food chewable tablet for weight management, which is composed of the following raw materials in parts by weight: 15 parts of citrus young fruit powder, 15 parts of microcrystalline cellulose, 30 parts of mannitol, 15 parts of oligofructose, 2.5 parts of citric acid, 2 parts of citrus flavor, 4 parts of magnesium stearate, and an appropriate amount of 75% by volume ethanol.

[0044] The preparation method of this embodiment is the same as that of embodiment 1.

[0045] The active ingredients of the chewable tablets prepared in this example were detected according to the method described in Example 1. The chewable tablets prepared in this example each had a total phenol content of 2.255 mg, a total flavonoid content of 1.995 mg, and a synephrine content of 4.829 mg. Example 5

[0046] This embodiment provides a citrus health food chewable tablet for weight management, which is composed of the following raw materials in parts by weight: 15 parts of citrus young fruit powder, 15 parts of microcrystalline cellulose, 30 parts of mannitol, 15 parts of oligofructose, 2 parts of citric acid, 2 parts of citrus flavor, 4 parts of magnesium stearate, and an appropriate amount of 75% by volume ethanol.

[0047] The preparation method of this embodiment is the same as that of embodiment 1.

[0048] The active ingredients of the chewable tablets prepared in this example were detected according to the method described in Example 1. The chewable tablets prepared in this example each contained 2.268 mg of total phenols, 2.007 mg of total flavonoids, and 4.856 mg of synephrine.

[0049] Comparative Example 1 This comparative example provides a citrus health food chewable tablet for weight management. The difference between this comparative example and Example 1 is only that citric acid is deleted, and the rest is the same as Example 1.

[0050] The active ingredients of the chewable tablets prepared in this example were detected according to the method described in Example 1. The chewable tablets prepared in this comparative example each had a total phenol content of 0.918 mg, a total flavonoid content of 0.812 mg, and a synephrine content of 1.965 mg.

[0051] Comparative Example 2 This comparative example provides a citrus health food chewable tablet for weight management. The difference between this comparative example and Example 1 is only that the amount of citric acid used is 0.5 parts, and the rest is the same as Example 1.

[0052] The active ingredients of the chewable tablets prepared in this example were detected according to the method described in Example 1. The chewable tablets prepared in this comparative example each had a total phenol content of 2.047 mg, a total flavonoid content of 1.811 mg, and a synephrine content of 4.383 mg.

[0053] Comparative Example 3 This comparative example provides a citrus health food chewable tablet for weight management. The difference between this comparative example and Example 1 is only that the amount of citric acid used is 10 parts, and the rest is the same as Example 1.

[0054] The active ingredients of the chewable tablets prepared in this example were detected according to the method described in Example 1. The chewable tablets prepared in this comparative example each had a total phenol content of 1.857 mg, a total flavonoid content of 1.643 mg, and a synephrine content of 3.975 mg.

[0055] From the comparison between Examples 1-5 and Comparative Examples 1-3, it can be seen that the contents of total phenol, total flavonoids, and synephrine in each chewable tablet prepared in the Examples of the present application are significantly higher than those in the chewable tablets prepared in Comparative Examples 1-3. Among them, the chewable tablet prepared in Example 3 has the highest content, with a total phenol content of 2.420 mg, a total flavonoid content of 2.140 mg, and a synephrine content of 5.180 mg.

[0056] According to the comparison of Examples 1-5, when the citrus young fruit powder is 15 parts, the microcrystalline cellulose is 15 parts, the mannitol is 30 parts, the oligofructose is 15 parts, and the citric acid is 1.5 parts, the total phenol, total flavonoids and synephrine contents of the prepared citrus health food chewable tablets are the highest. This is because microcrystalline cellulose, mannitol, oligofructose, and citric acid are compounded with the citrus young fruit powder in a specific ratio, which can retain the two components of citrus polyphenols and synephrine in the citrus young fruit to the greatest extent.

[0057] By comparing Example 1 with Examples 4 and 5, it can be seen that when the amount of citric acid is increased, the contents of total phenols, total flavonoids and synephrine in the young citrus fruits measured are significantly reduced, which shows that the flavoring agent of the present application not only improves the taste, but also the composite flavoring agent composed of citric acid and oligofructose is combined with the other components in a specific proportion, which plays a synergistic role and is helpful for the extraction of total phenols, total flavonoids and synephrine.

[0058] According to Comparative Example 1, due to the lack of citric acid, the total phenol, total flavonoids and synephrine contents in the chewable tablets were greatly reduced in Comparative Example 1. This may be because the addition of citric acid may change the pH value of the system and affect the extraction of active ingredients.

[0059] According to Comparative Examples 2 and 3, when the dosage of citric acid is too low or too high, the contents of total phenols, total flavonoids and synephrine in the chewable tablets will be greatly reduced.

[0060] Experimental example Experimental animals: 100 adult C57BL / 6J mice, weighing 18-22 g, were divided into 10 groups, with 10 mice in each group (half male and half female).

[0061] Trial Groups: Group 1: a citrus health food chewable tablet for weight management prepared in Example 1; Group 2: a citrus health food chewable tablet for weight management prepared in Example 2; Group 3: a citrus health food chewable tablet for weight management prepared in Example 3; Group 4: a citrus health food chewable tablet for weight management prepared in Example 4; Group 5: a citrus health food chewable tablet for weight management prepared in Example 5; Group 6: a citrus health food chewable tablet for weight management prepared in Comparative Example 1; Group 7: a citrus health food chewable tablet for weight management prepared in Comparative Example 2; Group 8: a citrus health food chewable tablet for weight management prepared in Comparative Example 3; Group 9: blank group; Group 10: control group; Experimental method: The blank group mice were fed with ordinary feed, the control group was fed with high-fat feed, and groups 1-8 were fed with high-fat feed and citrus young fruit health food (ground and mixed in feed at 10 mg / kg). The weight changes of mice were measured every week, and the experimental period was 30 days. The experimental results are shown in Table 1:

[0062] As can be seen from Table 1, the weight of mice fed with a high-fat diet mixed with the citrus young fruit health food provided in Examples 1-5 and Comparative Examples 1-3 was close to the initial weight of mice fed with ordinary diets, but after 30 days, the weight of mice in Groups 1-5 was significantly lighter than that of mice fed with a high-fat diet, indicating that the citrus young fruit health food provided in the examples of the present application can effectively inhibit the weight gain of mice and reduce fat accumulation. In addition, the weight of mice fed with a high-fat diet and the citrus young fruit health food provided in Comparative Examples 1-3 was significantly heavier than that of mice fed with a high-fat diet mixed with the weight loss tablets provided in Examples 1-5, indicating that the weight loss effect of the citrus young fruit health food provided in the examples of the present application is better.

[0063] The embodiments of the present application are described above in conjunction with the accompanying drawings, but the present application is not limited to the above-mentioned specific implementation methods. The above-mentioned specific implementation methods are merely illustrative and not restrictive. Under the guidance of the present application, ordinary technicians in this field can also make many forms without departing from the purpose of the present application and the scope of protection of the claims, all of which are within the protection of the present application.

Claims

1. A citrus young fruit health food for weight management, characterized in that: The invention comprises citrus young fruit powder, an excipient and a first corrective. According to weight proportion, the citrus young fruit powder is 15 parts; the excipient is 30-50 parts; and the first corrective is 16.5-27.5 parts.

2. The citrus young fruit health food for weight management according to claim 1, characterized in that: The excipients are microcrystalline cellulose and / or mannitol; the first flavoring agent is oligofructose and citric acid.

3. The citrus young fruit health food for weight management according to claim 1 or 2, characterized in that: In terms of weight, the excipient is composed of the following raw materials: 10-20 parts of microcrystalline cellulose and 20-30 parts of mannitol.

4. The citrus young fruit health food for weight management according to claim 1 or 2, characterized in that: In terms of weight, the first flavoring agent is composed of the following raw materials: 1.5-2.5 parts of citric acid and 15-25 parts of oligofructose.

5. The citrus young fruit health food for weight management according to claim 4, characterized in that: The invention comprises the following raw materials in parts by weight: 15 parts of citrus young fruit powder, 15 parts of microcrystalline cellulose, 30 parts of mannitol, 15 parts of oligofructose and 1.5 parts of citric acid.

6. The citrus young fruit health food for weight management according to claim 1, characterized in that: The invention also comprises a binder, wherein the binder is ethanol with a volume fraction of 75%.

7. The citrus young fruit health food for weight management according to claim 1, characterized in that: The invention also includes a lubricant, which is magnesium stearate and has an amount of 4 parts by weight.

8. The citrus young fruit health food for weight management according to claim 1, characterized in that: The invention also includes a second flavoring agent, which is citrus flavor, and the citrus flavor is 2 parts by weight.

9. The citrus young fruit health food for weight management according to claim 8, characterized in that: The health food is a chewable tablet.

10. The method for preparing the citrus young fruit health food for weight management according to claim 9, characterized in that: The following steps are involved: ① Preparation of citrus young fruit powder: Wash the citrus young fruit that meets the requirements in clean water, drain the water, slice it, freeze-dry it for 48 hours, crush it through a 100-mesh sieve, put it in a sealed container and store it in a -20℃ refrigerator for later use; ② Weigh the citrus young fruit powder, excipients, and the first flavoring agent according to the proportion, mix them evenly, and obtain a mixture; ③ The mixture prepared in step ② is added with a binder to make a soft material, granulate, and dry to obtain dry granules; ④ After the dry granules prepared in step ③ are uniformly mixed with the second flavor corrective and the lubricant, tableting is performed.

Images