Lipoprotein (a) determination kit and preparation method thereof

A lipoprotein and kit technology, applied in the fields of medicine and biochemistry, can solve problems such as radioactive pollution, low measurement accuracy, complicated operation, etc., and achieve good protection, accurate results, and good stability

Inactive Publication Date: 2016-11-09
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a kit for measuring lipoprotein (a) and its prepa...

Method used

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  • Lipoprotein (a) determination kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0074] Reagent R1:

[0075] MES buffer 100mmol / L

[0076] Bovine Serum Albumin 13 g / L

[0077] Polyethylene glycol-8000 30g / L

[0078] Sodium Benzoate 0.9 g / L

[0079] Rabbit anti-human monoclonal antibody 1.0g / L

[0080] The solvent is purified water

[0081] Reagent R2:

[0082] MES buffer 80mmol / L

[0083] Tween-80 1.5 mL / L

[0084] Bovine serum albumin 20 g / L

[0085] Sodium alginate 17g / L

[0086] Sodium Benzoate 0.9 g / L

[0087] Latex-coated anti-human lipoprotein (a) antibody 1g / L

[0088] Its solvent is purified water.

Embodiment 2

[0090] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0091] Reagent R1:

[0092] HEPES buffer 185 mmol / L

[0093] Trehalose 18 g / L

[0094] Polyethylene glycol-6000 20 g / L

[0095] Phenol 0.5 g / L

[0096] Goat anti-human polyclonal antibody 0.2g / L

[0097] The solvent is purified water

[0098] Reagent R2:

[0099] HEPES buffer 50 mmol / L

[0100] Tween-80 0.5 mL / L

[0101] Trehalose 14 g / L

[0102] Gum Arabic 25 g / L

[0103] Phenol 0.5g / L

[0104] Latex-coated anti-human lipoprotein (a) antibody 2g / L

[0105] Its solvent is purified water.

Embodiment 3

[0107] Kit preparation and method of use

[0108] 1. Preparation of latex-coated anti-human lipoprotein (a) antibody: Dilute polystyrene microspheres with a particle diameter of 120 nm to a mass concentration of polystyrene microspheres contained in 50 mmol / L MES buffer solution. 3% solution, and then add 0.5 mg 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride to each milliliter solution, react at 26°C for 2 hours, use centrifugation machine, centrifuge at 15000 rpm / min for 30 minutes, remove the supernatant, suspend the precipitate in 50 mmol / L MES buffer solution, use an ultrasonic disperser for ultrasonic dispersion, and then use a centrifuge at 15000 rpm / min Centrifuge for 30 minutes at a high speed, remove the supernatant, suspend the pellet in 50 mmol / L MES buffer, ultrasonically disperse, then add an equal volume of MES buffer containing anti-human lipoprotein (a) antibody while stirring, Mix and stir, and react for 2 hours at room temperature at 25°C, so t...

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Abstract

The invention discloses a lipoprotein (a) determination kit and a preparation method thereof. The kit comprises a reagent R1 and a reagent R2 which are liquid components and are mutually independent, the reagent R1 comprises a buffer solution, a stabilizer, an accelerator, an antiseptic, an anti-human rheumatoid factor antibody and purified water used as a solvent, and the reagent R2 comprises the buffer solution, a surfactant, the stabilizer, a suspending aid, the antiseptic, a latex coated anti-human lipoprotein (a) antibody and purified water used as a solvent. The preparation method comprises the following steps: preparing the reagents according to the component content; mixing a sample to be determined with the reagent R1 and the reagent R2 to fully react the sample to be determined with the reagent R1 and the reagent R2; determining the absorbance difference by using a fully-automatic biochemical analyzer after the reaction; and calculating the concentration of lipoprotein (a) in the sample according to the absorbance change value. The kit has high accuracy.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring lipoprotein (a) and a preparation method thereof. Background technique [0002] Lipoprotein (a) is a special independent plasma lipoprotein, which was discovered by Norwegian geneticist Berg in 1963 when he was studying the genetic variation of low-density lipoprotein. In the late 1980s, it was discovered that lipoprotein (a) was associated with atherosclerosis. [0003] Lipoprotein (a) is a lipoprotein rich in cholesterol. The core part is neutral lipid and apoB-100 molecules, and its periphery is surrounded by hydrophilic apo (a). The two are covalently disulfide bonded Connection; where apo (a) is a characteristic glycoprotein component of lipoprotein (a), mainly composed of a characteristic structure called Kringle, Kringle is composed of 80-114 amino acid residues, relying on three internal disulfides The keys are stable. [0004] Li...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N35/00
CPCG01N33/577G01N35/00
Inventor 蔡晓辉庄庆华吴铮徐运
Owner ANHUI IPROCOM BIOTECH CO LTD
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