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Kit for measuring procalcitonin

A technology of procalcitonin and a kit, which is applied in the fields of medicine and biochemistry, can solve problems such as high cost, low measurement accuracy, and pollution, and achieve the effects of convenient detection, good detection accuracy, and high detection sensitivity

Inactive Publication Date: 2016-10-12
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention discloses a kit for measuring procalcitonin aiming at the above problems, which overcomes the problems of high cost, pollution and low measurement accuracy

Method used

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  • Kit for measuring procalcitonin
  • Kit for measuring procalcitonin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0064] Reagent R1:

[0065] Tris buffer 80 mmol / L

[0066] Sodium chloride 200 mmol / L

[0067] Polyethylene glycol-6000 25 g / L

[0068] EDTA 40mmol / L

[0069] Tween-80 1.0 mL / L

[0070] Sodium azide 0.7g / L

[0071] Anti-human rheumatoid factor antibody 1.0 g / L

[0072] Its solvent is purified water.

[0073] Reagent R2:

[0074] Tris buffer 100 mmol / L

[0075] Bovine Serum Albumin 35 g / L

[0076] Sodium chloride 110 mmol / L

[0077] Sodium azide 0.65 g / L

[0078] Latex-coated anti-human procalcitonin monoclonal antibody 4g / L

[0079] Its solvent is purified water.

Embodiment 2

[0081] Reagent R1:

[0082] MES buffer 80 mmol / L

[0083] Sodium chloride 200 mmol / L

[0084] Polyethylene glycol-8000 25 g / L

[0085] EDTA 40mmol / L

[0086] Tween-80 1.0 mL / L

[0087] Sodium azide 0.7g / L

[0088] Anti-human rheumatoid factor antibody 1.0 g / L

[0089] Its solvent is purified water.

[0090] Reagent R2:

[0091] MES buffer 100 mmol / L

[0092] Bovine Serum Albumin 35 g / L

[0093] Sodium chloride 110 mmol / L

[0094] Sodium azide 0.65 g / L

[0095] Latex-coated anti-human procalcitonin monoclonal antibody 4g / L

[0096] Its solvent is purified water.

Embodiment 3

[0098] Kit preparation and method of use

[0099] 1. Preparation of latex-coated anti-human procalcitonin monoclonal antibody:

[0100] (a) Dilute latex particles with a particle size of 80nm to 4.0 g / L with 50 mmol / L MES buffer, add 1.0 mg of EDAC to each mL of solution, react at room temperature for 2 hours, and centrifuge at 20,000 rpm Centrifuge at high speed for 30 minutes, remove the supernatant, suspend the pellet in 50 mmol / L MES buffer, and disperse by ultrasonic;

[0101] (b) Centrifuge the product of step (a) at 20,000 rpm for 30 minutes in a centrifuge, discard the supernatant, and suspend the precipitate in 50 mmol / L MES buffer, so that the final concentration of latex particles is 4g / L, Ultrasonic dispersion, while stirring, add an equal volume of MES diluent containing anti-human procalcitonin antibody, mix and stir, react at room temperature for 2 hours, and the final concentration of latex particles is 4.0g / L.

[0102] (c) Centrifuge the product of step (b) ...

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Abstract

The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring procalcitonin. The purpose of the present invention is to solve the problem of complex operation and low measurement accuracy in the detection process of fixed procalcitonin in the prior art. The double reagent used in the present invention firstly mixes the reagent R1 containing the anti-human rheumatism factor antibody with the sample , so that the rheumatoid factor antigen in the sample combines with the anti-human rheumatoid factor antibody in reagent R1 to form an antigen-antibody complex, so as to achieve the purpose of clearing the rheumatoid factor antigen; The reagent R2 is mixed with the above sample, and the procalcitonin antigen is specifically combined to form an insoluble polystyrene microsphere-antigen-polystyrene microsphere particle complex emulsion, which produces a certain turbidity, and at a certain wavelength The content of procalcitonin detected in the sample can be measured by performing turbidimetric determination. The invention has the advantages of high accuracy, no pollution, convenient operation and the like.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring procalcitonin. Background technique [0002] Procalcitonin (PCT) is a protein whose plasma levels are elevated in severe bacterial, fungal, and parasitic infections, as well as in sepsis and multiorgan failure. PCT will not increase in autoimmunity, allergy and viral infection. Local limited bacterial infection, minor infection and chronic inflammation do not cause it to be elevated. Bacterial endotoxins played a crucial role in the induction process. [0003] PCT reflects the active degree of systemic inflammatory response. Factors that affect PCT levels include the size and type of infected organ, the type of bacteria, the degree of inflammation, and the status of the immune response. Elevated levels of PCT occur in severe shock, systemic inflammatory response syndrome, and multiple organ dysfunction syndrome, even in the absence of ba...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/68
CPCG01N33/577G01N33/6803
Inventor 蔡晓辉庄庆华吴铮徐运
Owner ANHUI IPROCOM BIOTECH CO LTD
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