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A technology for rheumatoid factor and rheumatoid factor, which is applied in the fields of medicine and biochemistry, can solve the problems of low measurement accuracy and complicated operation of rheumatoid factor, and achieves the effects of good detection accuracy, convenient detection and high detection accuracy.
Inactive Publication Date: 2016-08-31
ANHUI IPROCOM BIOTECH CO LTD
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[0005] The purpose of the present invention is to solve the problems of complex operation and low measurement accuracy in the detection process of rheumatoid factor in the prior art, and provide a kit for measuring rheumatoid factor
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Embodiment 1
[0069] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein
[0086] 1. Dilute the latex particles with a particle size of 120nm to 2 g / L with 50 mmol / L MES buffer solution, add 1.0 mg of EDAC to each mL solution, react at room temperature for 2 hours, and spin in a centrifuge at 15000 rpm Centrifuge for 30 minutes, remove the supernatant, suspend the precipitate in 50 mmol / L MES buffer, and disperse by ultrasonic; then centrifuge at 15000 rpm in the centrifuge for 30 minutes, discard the supernatant, and suspend the precipitate in 50 mmol / L In MES buffer of 1 L, the final concentration of latex particles was 4%, ultrasonically dispersed, and an equal volume of MES dilution solution containing anti-human rheumatism factor antibody was added while stirring, mixed and stirred, and reacted at room temperature for 2 hours, and the final concentration of latex particles was 2 g / L; then centrifuge at 15,000 rpm for 30 minutes in a centrifuge, discard the supernatant, suspend the pellet in 50 mmol / L ME...
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Abstract
The invention relates to the technical field of medicine and biochemistry and particularly relates to a kit for determining rheumatoid factors, aiming at solving the problems in the prior art that a rheumatoid factor detection process is complicated to operate and the determination accuracy is low. Aiming at solving the technical problems above, the technical scheme provided by the invention is as follows: a kit for determining hyaluronic acid is provided, wherein the kit is composed of double liquid reagents R1 and R2, the reagent R1 is prepared from the following components: 30mmol / L-230mmol / L of a Tris buffering solution, 2.0mL / L-6.0mL / L of Tween-80, 5g / L-15g / L of polyethylene glycol-8000, 30mmol / L-50mmol / L of EDTA (Ethylene Diamine Tetraacetic Acid), 0.4g / L-1.0g / L of sodium azide and a solvent which is a purified water; the reagent R2 is prepared from the following components: 50mmol / L-150mmol / L of a Tris buffering solution, 5mmol / L-25mmol / L of glycerol, 0.4g / L-1.0g / L of sodium azide, 20g / L-60g / L of bovine serum albumin, 0.5%-4.0% of an emulsion-coated anti-human rheumatoid factor antibody and a solvent which is purified water. The kit for determining the rheumatoid factors has the advantages of high accuracy, convenience for operation and the like.
Description
technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring rheumatoid factor. Background technique [0002] Rheumatoid factor (rheumatoid factor, RF) can be divided into five types: IgM, IgA, IgG, IgD, and IgE. It is a type of autoantibody against the antigenic epitope on the Fc fragment of IgG in rheumatoid arthritis serum. Patients with rheumatoid factor positive More associated with extra-articular manifestations, such as subcutaneous nodules and vasculitis. The positive rate of IgM type RF was 60%-78%. [0003] Rheumatoid factor (RF) is found in the serum of patients with rheumatoid arthritis (RA). It is an autoantibody that targets denatured IgG. It mainly exists in the serum and joint fluid of patients with rheumatoid arthritis. It is an anti-denatured IgG antibody, which is of the IgM type. Can bind to IgG Fc fragment. RA patients and about 50% of healthy people have RF-producing B cell c...
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