44results about How to "Reduce signal background" patented technology

The invention discloses a method for detecting fungaltoxin through multiple signals. The method comprises the following steps: 1) combining fungaltoxin with aptamer: adding a to-be-detected sample after the reaction of the fungaltoxin aptamer and complementary sequence thereof, thereby acquiring a mixed solution A; 2) amplifying a digestion auxiliary signal: reacting the mixed solution A with restriction enzyme, thereby acquiring a mixed solution B; 3) preparing a guanine tetramer structure: reacting the mixed solution B with tail-end deoxynucleotide transferase and deoxyribonucleoside triphosphate and reacting with ligand molecules, thereby acquiring a mixed solution C; and 4) detecting and analyzing: performing catalytic oxidation reaction on the mixed solution C and different substrates, generating ultraviolet, fluorescent and chemiluminescent signals and calculating the content of fungaltoxin in the to-be-detected sample according to the relation of the response strength of all the signals and the fungaltoxin concentration. The method disclosed by the invention has the characteristics of quick and simple operation and treatment, short detection time, marker-free effect, low cost, high precision, high sensitivity, and the like.

The invention discloses a chemiluminescence detection kit. The kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 is prepared from a buffer solution, magnetic particles, a first antibodyof a biotin-labeled to-be-detected antigen, and polyhydric alcohol; and the reagent 2 is prepared from a buffer solution, a second antibody of an acridinium ester-labeled to-be-detected antigen, andpolyhydroxy saccharide. The chemiluminescence detection kit is high in sensitivity and good in accuracy, and can lower the detection cost.

The invention discloses an adrenocorticotropic hormone (ACTH) chemiluminescence detection kit, which comprises a reagent (1) and a reagent (2). The reagent (1) comprises a buffer solution, magnetic particles, a biotin labeled anti-ACTH monoclonal antibody, and polyhydric alcohol. The reagent (2) comprises a buffer solution, an acridinium ester labeled anti-ACTH monoclonal antibody and polyhydroxylsaccharides. The provided kit has the advantages of good stability, high sensitivity, good accuracy, simple and convenient operation, and low cost, and is not influenced by rheumatoid factors.

The invention discloses a preparation method of electrochemical sensor for sulfonamide molecule, and belongs to the technical field of new nano functional materials and biosensor. The method firstly prepares a nickel hydroxide nanosheet array on a disposable throwable electrode, and uses the large specific surface area and a high activity hydroxyl functional group and an amino functional group ofpolydopamine to sequentially prepare a polydopamine film containing an electron mediator and a molecularly imprinted polymer taking a sulfonamide molecule as a template molecule directly on the nickelhydroxide nanosheet array by adopting an in-situ growth method, after eluting the template molecule, the position of the original template molecule becomes a hole, that is, the molecularly imprintedpolymer eluting the template molecule, thus, an electrochemical sensor for the sulfonamide molecule is prepared.

The invention discloses a preparation method of a macrolide antibiotic sensor based on iron nitride, and belongs to the technical field of new nano functional materials and chemical biosensors. The method comprises the following steps: firstly preparing an array of iron nitride nanosheets on a disposable electrode, directly preparing a polydopamine film and a molecularly imprinted polymer using the macrolide antibiotic molecule as a template molecule coated with luminol in situ successively on the array of iron nitride nanosheets one after another by adopting an in-situ growth method and utilizing a large specific surface area and a high adsorption activity for amidogen, and eluting the molecularly imprinted polymer of the template molecule after the template molecule is eluted and the original position of the template molecule became a hole, thereby preparing a macrolide antibiotic sensor based on iron nitride.

The invention discloses a preparation method for a tetracycline antibiotic electrochemiluminescence sensor, and belongs to the technical field of novel nanometer function materials and biosensing analysis. The preparation method firstly prepares a nickel-iron bimetallic oxide nanosheet array on a disposable electrode, the large specific surface area of the nickel-iron bimetallic oxide nanosheet array, high adsorption activity for an amino and the amino functional group of polydopamine are used, an in-situ growth method is adopted to successively directly preparing a polydopamine film and a molecularly imprinted polymer which coats luminol in an in-situ way and takes tetracycline antibiotics as a template molecule on the nickel-iron bimetallic oxide nanosheet array, and after the template molecule is eluted, the position of the original template molecule becomes a cavity, i.e., the molecularly imprinted polymer of the template molecule is eluted. Therefore, the tetracycline antibiotic electrochemiluminescence sensor finishes being prepared.

The invention discloses a thyroglobulin chemical luminescence detection kit. The kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 is prepared from buffer liquor, magnetic particles, biotin-labelled anti-thyroglobulin monoclonal antibody and polyhydroxy-alcohol; and the reagent 2 is prepared from buffer liquor, an acridinium ester labeled anti-thyroglobulin monoclonal antibody and polyhydroxyl sugar. The thyroglobulin chemical luminescence detection kit is high in sensitivity, is good in accuracy, and can reduce detection cost.

The invention discloses a preparation method of a nitrofuran antibiotic sensor, and belongs to the technical field of new nano functional materials and biosensing analysis technology. The method comprises the following steps: firstly preparing an array of cobalt-nickel bimetal layered hydroxide nanosheets on a disposable electrode, directly preparing a polydopamine film containing an electron mediator and a molecularly imprinted polymer using the nitrofuran antibiotic as a template molecule successively on the array of cobalt-nickel bimetal layered hydroxide nanosheets one after another by adopting an in-situ growth method and utilizing a large specific surface area and high active hydroxyl functional groups, and eluting the molecularly imprinted polymer of the template molecule after thetemplate molecule is eluted and the original position of the template molecule became a hole, thereby preparing a nitrofuran antibiotic sensor.

The invention discloses a fabrication method of a tetracycline antibiotic molecular imprinting electrochemical sensor, and belongs to the technical field of novel nanometer functional material and chemical biological sensor. A nickel-iron thermometal nitride nanosheet array is fabricated on a disposable throwable electrode, an electronic medium-containing polydopamine thin film and a molecular imprinting polymer taking ampicillin as a template molecule polymer are directly and sequentially fabricated on the nickel-iron thermometal layered hydroxide nanosheet array by an in-situ growth method according to large specific surface area, high-activity hydroxyl functional group and amino functional group of polydopamine. After the template molecule is eluted, the original position of the template molecule is changed to holes, and the molecule imprinting polymer of the template molecule is eluted. Therefore, the tetracycline antibiotic molecular imprinting electrochemical sensor is fabricatedand completed.

The invention discloses a hepatitis B PreS1 antigen chemiluminescence detection kit. The kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 is prepared from buffer liquid, magnetic particles, biotin marked anti-hepatitis B PreS1 antigen monoclonal antibody and polyhydroxy alcohol; the reagent 2 is prepared from buffer liquid, acridinium ester marked anti-hepatitis B PreS1 antigen monoclonal antibody and polyhydroxy saccharide. The kit has the advantages that in the use process, the signal background is low; the signal intensity is obviously improved; the sensitivity, the accuracyand the repeatability of the kit are obviously improved.

The invention belongs to the field of biotechnologies and discloses a detection kit for completely and homogeneously determining glucagon and a method thereof. The detection kit utilizes a spatial neighborhood chemiluminescence technology to achieve complete and homogeneous detection of glucagon and is based on a double-sandwich technique, two monoclonal antibodies are bound to antigenic determinants of glucagon molecules respectively, a calibrator or a sample to be tested, a horse radish peroxidase-labeled anti-human glucagon antibody, a 9,10-dihydroacridine-labeled anti-human glucagon antibody are added together into a microplate, the two monoclonal antibodies and the antigen in the sample form an antibody-antigen-antibody sandwich complex without coating after incubation, the washing process is omitted, a trigger is added after an antioxidant is added and evenly mixed, a luminescence intensity value in a period of time is immediately continuously detected, and the content of the glucagon in the sample to be tested is calculated. The detection kit has the advantages of high stability, rapid and convenient detection, high sensitivity and strong specificity.

The invention discloses a preparation method for a cobalt-based nitride sensor for detecting praziquantel, and application, and belongs to the technical field of novel nanometer function materials andchemical biosensors. The preparation method firstly prepares a cobalt nitride nanosheet array on a disposable electrode, the large specific surface area of the cobalt nitride nanosheet array and highadsorption activity for an amino are used for successively directly preparing a polydopamine film containing an electronic mediator and a molecularly imprinted polymer which takes a praziquantel molecule as a template molecule on the cobalt nitride nanosheet array through an in-situ growth method, and after the template molecule is eluted, the position of the original template molecule becomes acavity, i.e., the molecularly imprinted polymer of the template molecule is eluted. Therefore, the cobalt-based nitride sensor for detecting praziquantel finishes being prepared.

The invention discloses a method for the ultra-sensitive detection of kanamycin based on a DNA/Ni-Fe LDO cubic network structure and belongs to the technical field of biological detection. The method comprises the following operation steps of: kanamycin is detected through the formation of a three-dimensional Ni-Fe LDO/DNA network triggered by aptamer high-specificity recognition; XPS analysis proves that Ni and Fe elements in the Ni-FeLDO are in coordination binding with G-quadruplex on the surface of the Ni-FeLDO, and electrons on the G-quadruplex are transferred into the Ni and Fe elements in the Ni-FeLDO with higher peroxidase-like catalytic activity in the catalytic oxidation process; and therefore, the detected limit of detection (LOD) is reduced to 0.31 fM (S/N = 3). In addition, the content of residual kanamycin in actual milk and urine samples can be accurately detected; and the method has a potential application value in detection of kanamycin residues in an actual sample.

The invention relates to a DAPI redyeing solution and a preparation method and application thereof. The DAPI redyeing solution is mainly prepared from p-phenylenediamine, Triton X-100, formamide, DAPI and glycerinum. Every 1mL of the DAPI redyeing solution is prepared from 10mg of p-phenylenediamine, 100 microliters of PBS buffer solution with pH of 9.0, 0.1-1.5 microliters of TritonX-100, 10-150 microliters of formamide, 250ng of DAPI, and 600-800 microliters of glycerinum. An existing DAPI redyeing solution is improved, the signal background is reduced by adding 1% Triton-X-100 and formamide in a certain proportion, the signal-to-noise ratio is increased, and clinical interpretation is convenient. The DAPI redyeing solution is applied to fluorescence in-situ hybridization, a more accurate experiment result can be obtained, and a more reliable diagnosis basis is provided for clinicians.