Kit for determining immunoglobulin A and preparation method thereof

A technology of immunoglobulin and human immunoglobulin, which is applied in the fields of medicine and biochemistry, can solve the problems of low measurement accuracy and complicated operation, and achieve the effect of simple operation and improved accuracy

Inactive Publication Date: 2016-08-31
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a kit for measuring immunoglobulin A and it

Method used

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  • Kit for determining immunoglobulin A and preparation method thereof
  • Kit for determining immunoglobulin A and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0072] Reagent R1:

[0073] Tris buffer 130 mmol / L

[0074] Sodium chloride 200 mmol / L

[0075] Polyethylene glycol-2000 25g / L

[0076] Sodium azide 0.7 g / L

[0077] Goat anti-human polyclonal antibody 0.5g / L

[0078] The solvent is purified water

[0079] Reagent R2:

[0080] MES buffer 140 mmol / L

[0081] Bovine serum albumin 20 g / L

[0082] Phenol 0.7 g / L

[0083] Latex coated anti-human immunoglobulin A antibody 8g / L

[0084] Its solvent is purified water.

Embodiment 2

[0086] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0087] Reagent R1:

[0088] MES buffer 30mmol / L

[0089] Potassium chloride 500 mmol / L

[0090] Polyvinylpyrrolidone 10g / L

[0091] Phenol 0.9 g / L

[0092] Rabbit anti-human monoclonal antibody 0.2 g / L

[0093] The solvent is purified water

[0094] Reagent R2:

[0095] Tris buffer 200 mmol / L

[0096] Glycerin 15 g / L

[0097] Sodium azide 0.6 g / L

[0098] Latex-coated anti-human immunoglobulin A antibody 1g / L

[0099] Its solvent is purified water.

Embodiment 3

[0101] Kit preparation and method of use

[0102] 1. Preparation of latex-coated anti-human immunoglobulin A antibody: first dilute polystyrene microspheres with a particle size of 100 nm with 50 mmol / L MES buffer to obtain a mass concentration of polystyrene microspheres of 3% solution, then add 1.0 mg of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride in every milliliter of solution, and react for 1 hour under the condition of 26 ℃, react After completion, add 2,2'-thionickel, use a centrifuge, centrifuge at a speed of 25000 rpm / min for 30 minutes, remove the supernatant, then dilute the precipitate with 50 mmol / L MES buffer, and then in Perform ultrasonic dispersion on an ultrasonic disperser, then use a centrifuge to centrifuge at a speed of 25,000 rpm / min for 30 minutes, remove the supernatant, and then dilute the precipitate with 50 mmol / L MES buffer until the solution contains polystyrene When the mass concentration of microspheres is 2%, then add anti-hum...

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Abstract

The invention discloses a kit for determining immunoglobulin A and a preparation method thereof. The kit comprises double liquid components including a reagent R1 and a reagent R2 which are independent to each other, wherein the reagent R1 is composed of a buffering solution, inorganic salt ions, a coagulant, a preservative and an anti-human rheumatoid factor antibody; and the reagent R2 is composed of a buffering solution, a stabilizer, a preservative and an emulsion-coated anti-human immunoglobulin A antibody. The preparation method comprises the following steps: preparing the reagents according to the content of the components; mixing a sample to be detected with the reagent R1 and the reagent R2 to have a sufficient reaction; determining a reacted absorbance difference value by utilizing a full-automatic biochemical analyzer; and calculating the concentration of the immunoglobulin A in a sample according to an absorbance change value. The kit gas has the advantages of high detection accuracy and the like.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring immunoglobulin A and a preparation method thereof. Background technique [0002] Immunoglobulins are divided into five classes, namely immunoglobulin G, immunoglobulin A, immunoglobulin M, immunoglobulin D and immunoglobulin A, and their main function is to react with antigen and antibody to generate antigen-antibody complexes , so as to block the harm of pathogens to the body and make pathogens lose their pathogenic effects. Measles (commonly known as rubella), etc. Immunoglobulin preparations can enhance the anti-virus ability of the human body and can be used as medicine. For example, injection of gamma globulin preparations extracted from human serum or human placenta can prevent and treat infectious diseases such as measles and infectious hepatitis. [0003] The content of immunoglobulin A in normal human serum is second only to immuno...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N21/31
CPCG01N33/6854G01N21/31
Inventor 蔡晓辉庄庆华吴铮徐运
Owner ANHUI IPROCOM BIOTECH CO LTD
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