Method for preparing methane-oxidizing bacteria on large scale

Abstract

The invention relates to a method for preparing methane-oxidizing bacteria on a large scale. The method includes the following steps: 1), putting the methane-oxidizing bacteria and inorganic salt into a reactor, and then injecting methane and oxygen into the reactor; 2), crushing the added gases into tiny bubbles by a crushing device in the reactor to enable the bubbles to be blended in liquid in the reactor and flow along the reactor internally together with the liquid, and preforming a fermentation process; 3), after the fermentation process is finished, determining completion of the fermentation process, performing initial condensation of a product in the reactor, and quickly cooling and drying the product after heating to obtain an initial product. The method has the advantages that the method mainly aims at preparation for methane-oxidizing bacteria production of large-scale reaction devices, the methane-oxidizing bacteria are cultivated by the devices such as the reactor, water solubility of the gases such as the methane is improved through measures such as bubble crushing, reaction efficiency is improved through addition of inorganic salt cultivation media, and accordingly, the method can be suitable for large-scale production application.

Description

technical field [0001] The invention relates to a method for preparing methane-oxidizing bacteria, which belongs to the technical field of enzymology or microbiology devices. Background technique [0002] Methanotrophs are special microorganisms that use methane as the only carbon source and energy source. Methanotrophs have important application value in natural carbon cycle and industrial biotechnology. The use of methane to produce single-cell protein began in the 1970s. In 1970, the British Shell Company established a natural gas protein test device with an annual output of 300 tons in the UK. In 1974, Shell announced the establishment of a larger test device in the UK. It is also preparing to prepare a development plan for a device with an annual output of 100,000 tons in Amsterdam, the Netherlands. But in 1976, Shell decided to stop the development and postpone it indefinitely, for what it came down to at the time: low prices for soybeans and corn. In 1999, Statoil a...

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Problems solved by technology

[0003] However, due to the low solubility of methane in aqueous solution, the efficiency of methane-oxidizing bacteria in utilizing methane is low, resulting in slow growth rate of methane-oxidizing bacteria and low cell concentration. Therefore, it has become a bottleneck for the industrial application of methane-oxidizing bacteria. Enhancing gas-liquid mass transfer is an established One of the keys to high-density and rapid cultivation of methanotrophs. The traditional solution is to increase the gas velocity and agitation rate, but increasing the gas velocity and agitation rate will lead to a large amount of foam on the one hand and reduce the utilization efficiency of the reactor. On the other hand, it will consume A lot of power, more cost

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