Method for preparing methanol from methane by biological catalysis

A technology for biocatalyzing methane and methanol, which is applied in the direction of fermentation, can solve the problems of reducing activity and stability, destroying the physiological structure of cells, etc., and achieves the effect of low reaction cost

Inactive Publication Date: 2005-02-16
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, blocking agents will inevitably destroy the physiological structure of cells, reduce their activity and stability, and this damage is irreversible

Method used

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  • Method for preparing methanol from methane by biological catalysis
  • Method for preparing methanol from methane by biological catalysis
  • Method for preparing methanol from methane by biological catalysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Methylomonas sp.GYJ3 was cultured, centrifuged after harvest, and added to a 100-mL Erlenmeyer flask containing 20 mL of a reaction solution containing the following components, in which the cells of Methylomonas sp.GYJ3 were contained The concentration is (5-40) mg / ml:

[0031] Disodium hydrogen phosphate at a concentration of 0-200 mmol / L,

[0032] Sodium dihydrogen phosphate at a concentration of 0-200 mmol / L,

[0033] Magnesium chloride at a concentration of 2-8 mmol / L,

[0034] pH=6.5-7.5.

[0035] One bottle was sealed and 60mL of air was drawn out, 20mL of methane and 20mL of oxygen were added, and the remaining gas phase was equilibrated with nitrogen as a control; 60mL of air was drawn out after one bottle was sealed, and 18mL of methane, 24mL of carbon dioxide and 18mL of oxygen were added; one bottle was added with a concentration of 2 mmol / L of ethylenediaminetetraacetic acid (EDTA), draw out 60mL of air after sealing, add 20mL of methane and 20mL of oxyg...

Embodiment 2

[0039] Others are the same as in Example 1, 32°C, 0.6Mpa, methane, oxygen, and carbon dioxide are mixed and charged into the membrane reactor at a volume ratio of 3:3:4, each batch reaction is 5-7h, and the cells are recovered after the reaction is completed. next batch of responses. Take 0.5mL of solution from the membrane reactor in each batch, detect the product methanol by gas chromatography, and then compare the change trend of its output. The results are shown in Table 2:

[0040]

[0041] It is found that methanol accumulates when the reaction system contains a certain amount of carbon dioxide, and the amount of methanol accumulation is maintained at a high and stable level; using ethylenediaminetetraacetic acid (EDTA) as a blocking agent can cause methanol accumulation, but the cell operation is stable A rapid decline in nicotinic 2 ) is rapidly consumed, and this consumption is irreversible in this reaction system.

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Abstract

The invention publishes a kind of biology method to prepare methanol using methane catalysis. The method adopts the technology as follows. Fist, bacterial cells are oxidized by methane and the outcome is as catalyst. Second, add mixed gas of methane, carbon dioxide and oxygen to membrane reactor and start up reaction under the condition of 32-40deg.C and 0-1.2MPa. after the reaction the aqueous solution containing methanol can be gotten. The invention solves the problems that cytoactive will depress because of the consumption of NADH lead by chemical inhibitor. In this method, the reaction can continue to make sure that coenzymes NADH can rebirth in situ in the reaction system.

Description

technical field [0001] The present invention relates to a method for biocatalyzing methane to prepare methanol, more particularly, the present invention relates to a method for biocatalyzing methane to methanol by methane oxidizing bacteria. technical background [0002] The selective oxidation of methane to methanol or other substances is one of the topics that arouses great interest among researchers. Methane can be used as an efficient fuel and chemical raw material and is abundant in reserves. If it can be used effectively, it can not only slow down today's energy crisis, but also reduce environmental pollution, which is in line with today's environmental protection theme. So far, there is no chemical catalyst that can directly oxidize methane to methanol in one step. These catalytic reactions not only require multi-step reactions, but also require a high temperature of 900 °C, which is not cost-effective in energy, and the reaction selectivity and conversion rate are v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/04
Inventor 崔俊儒辛嘉英夏春谷李树本
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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