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Preparation method for methane-oxidizing bacteria liquid, refuse landfill covering materials and refuse landfill methane emission reduction method

A methane oxidizing bacteria, landfill technology, applied in the direction of landfill technology, bacteria, solid waste removal, etc., can solve the problem of slow onset of overburden, to extend the service life, rapid onset, control The effect of methane emissions

Inactive Publication Date: 2014-01-08
SUZHOU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the covering layer obtained by this method takes effect slowly, and the methane oxidation efficiency can only reach 70%-80% after one month of covering

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0021] The invention provides a method for preparing a methane-oxidizing bacteria liquid, comprising the following steps:

[0022] The mineralized waste is mixed with the leachate, and cultivated under the condition of feeding a mixed gas of methane and oxygen to obtain a methane-oxidizing bacteria liquid, and the methane oxidation rate of the mineralized waste exceeds 50%.

[0023] In the present invention, the mineralized garbage is used as the source of methane-oxidizing bacteria strains, and the leachate is used as a culture substrate to mix and cultivate methane-oxidizing bacteria. In order to adapt, and the obtained methanotrophic bacteria liquid works quickly, the oxidation efficiency of methane in landfill gas is higher, and the methane oxidation rate can reach more than 84% in 7 days of coverage.

[0024] The present invention uses mineralized garbage as the source of methane-oxidizing bacteria, and the methane oxidation rate of the mineralized garbage exceeds 50%. W...

Embodiment 1

[0042] The 10-year-old mineralized waste raw material from the Shanghai Laogang domestic waste landfill was taken, and the fine material with a particle size of 0.5 mm to 4 cm and a moisture content of 26 wt% was obtained after removal of impurities and sieving. Put the mineralized waste into a serum bottle, and inject CH with a volume ratio of 1:1 4 and O 2 The mixed gas was domesticated at 37°C for 7 days. At this time, the methane oxidation rate of the mineralized waste reached 56.8%, and the cultivation was stopped.

[0043] Add the 10-year-old leachate from Shanghai Laogang domestic waste landfill to the mineralized waste to obtain a mixed solution, the proportion of which is 1g of mineralized waste: 100mL leachate, and the pH value of the mixed solution is 7.6; the mixed solution Put it into a serum bottle and inject CH with a volume ratio of 1:1 4 and O 2 The mixed gas was sealed and cultured on a shaking table at 35°C. Cultivate for 3 days, at this time, the bacter...

Embodiment 2

[0049] The raw material of 16-year-old mineralized waste from the Shanghai Laogang domestic waste landfill was removed and screened to obtain fine material with a particle size of 0.5 mm to 4 cm and a moisture content of 23%. Put the mineralized waste into a serum bottle, and inject CH with a volume ratio of 1:1.3 4 and O 2 Acclimate at 37°C for 7 days. At this time, the methane oxidation rate of the mineralized waste reaches 55%, and the cultivation is stopped.

[0050] The leachate mineralization bed treatment tail water of the Shanghai Laogang domestic waste landfill was added to the mineralized waste to obtain a mixed solution, the proportion of which was 0.5g mineralized waste: 100mL leachate, and the pH value of the mixed solution was 7.5; Put the mixture into a serum bottle, and inject CH with a volume ratio of 1:1.3 4 and O 2 Afterwards, it was sealed and cultured on a shaking table at 35°C. Cultivate for 3 days, at this time, the bacteria concentration OD of the m...

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Abstract

The invention discloses a preparation method for methane-oxidizing bacteria liquid. The preparation method comprises the following steps that mineralized refuse is mixed with leachate, cultivation is conducted on the condition that mixed gas of methane and oxygen is input, the methane-oxidizing bacteria liquid is obtained, and the methane oxidization rate of the mineralized refuse exceeds 50 %. The invention provides refuse landfill covering materials and a refuse landfill methane emission reduction method. The mineralized refuse is used as the source of the methane oxidation bacteria strain, the leachate is used as a culture medium to culture the methane oxidation bacteria in a mixed mode, the cultivation system is close to the environment of the landfill, oxidization efficiency of the methane in landfill gas is higher, and the methane oxidation rate can exceed 84 % when covering is conducted for 7 days.

Description

technical field [0001] The invention belongs to the technical field of environmental protection materials, and in particular relates to a method for preparing a methane-oxidizing bacteria liquid, a covering material for a landfill and a method for reducing methane emission in a landfill. Background technique [0002] Landfill is a place where garbage is piled up under the sanitary landfill method. It is widely used in China because of its low cost and good sanitation. For old and small and medium-sized domestic waste landfills, it is difficult to recycle methane due to the low concentration or amount of methane produced in the landfill gas. Control and reduce methane release. Studies have shown that compost has a good methane oxidation effect and can be used to prepare bio-covers for landfills to improve the methane oxidation capacity of the covers. However, for most domestic waste landfills in my country, it is inconvenient to obtain compost, and it is necessary to find m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09B1/00
CPCY02W30/30
Inventor 梅娟陈重军钱飞跃王建芳沈耀良
Owner SUZHOU UNIV OF SCI & TECH
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