Method for removing methane in environment

A methane and environmental technology, applied in the field of methane removal in the environment, can solve the problems of unstable function, slow growth of pure culture of methane oxidizing bacteria, and few pure bacteria resources of methane oxidizing bacteria, and achieve easy operation, favorable industrial application, and high capacity strong effect

Inactive Publication Date: 2010-03-17
WUXI RES INST OF APPLIED TECH TSINGHUA UNIV +1
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Problems solved by technology

The pure culture of methanotrophs grows very slowly, the function is unstable, and the pure bacteria resources of methanotrophs are less

Method used

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  • Method for removing methane in environment
  • Method for removing methane in environment
  • Method for removing methane in environment

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Example 1. Removal of methane in the environment

[0021] 1. Soil sample collection and medium preparation

[0022] Soil samples: Sampling from the fourth mine of a high gas coal mine in Xinfeng Mineral Bureau of Henan Province. The samples were taken from the 5cm deep soil at the outlet of the coal mine. The basic properties of the soil samples are shown in Table 1.

[0023] Table 1 Properties of soil samples from Xinfeng coal mine (Sampling date: October 9, 2006)

[0024]

[0025] The medium used for cultivating and screening the methane-oxidizing mixed bacteria is NMS medium, and the preparation method is as follows:

[0026] Solution A (per liter): KNO 3 10g, MgSO 4 ·7H 2 O 10g, CaCl 2 ·2H 2 O 2g, deionized water dilute to 1L;

[0027] Solution B (per liter): Fe-EDTA 0.38g, NaMoO 4 0.26g, FeSO 4 0.5g, MnCl 2 ·4H 2 O 0.02g, CoCl 2 ·6H 2 O 0.05g, H 3 BO 3 0.015g, Na-EDTA 0.25g, ZnSO 4 ·7H 2 O 0.4g, NiCl 2 ·6H 2 O 0.01g, CuSO 4 ·5H 2 O 0.025g, deionized water dilute to 1L;

[...

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Abstract

The invention discloses a method for removing methane in the environment, comprising the following steps: 1) collecting soil sample of a coal mine or a refuse landfill; 2) adding the soil sample of the step 1) into an NMS culture medium, and culturing in a sealed bottle; when in initial period, leading the volume ratio among the NMS culture medium, the methane and air in the sealed bottle to be (5-30): (5-40): (30-80); replacing the methane into the sealed bottle every 24-72h, leading the volume ratio among the NMS culture medium, the methane and air in the sealed bottle to be (5-30): (5-40):(30-80); 3) inoculating the culture solution of the step 2) into a new NMS culture medium for passaging every 8-12 days; leading the volume ratio between the culture solution of the step 2) and the NMS culture medium to be (5-15): (95-85); carrying out passage for more than ten generations, and obtaining mixed methane-oxidizing bacteria; and 4) culturing the mixed methane-oxidizing bacteria obtained from the step 3), and removing the methane in the environment. The method for removing the methane in the environment has simple operation, strong methane removing capability and wide application scope, and has broad application prospect for removing coal-mine gas, reducing emission of landfill gas of the refuse landfill and the like.

Description

Technical field [0001] The invention relates to a method for removing methane in the environment. Background technique [0002] Methane is an important chemical raw material and chemical energy. It is also the second greenhouse gas in the earth’s atmosphere after carbon dioxide. The greenhouse effect caused by methane is 20-30 times that of carbon dioxide of the same quality (Cicerone RJ, Oremland R S. Biogeochemical aspects) of atmospheric methane. Globle Biogeochem Cycles, 1998, 2:299-327.). According to estimates, in the past 200 years, the content of methane in the atmosphere has increased at an average annual rate of 1%, and has shown a significant upward trend in the past one or two years. According to the IPCC (United Nations Intergovernmental Panel on Climate Change) report statistics, the total global methane emissions in 1990 were about 5-8 billion tons, of which 1 / 2 was due to the development and utilization of fossil fuels (such as coal mining, oil extraction, etc.),...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D53/84B01D53/72C12N1/20C12R1/26C12R1/07C12R1/01
CPCY02A50/20Y02P20/59
Inventor 邢新会江皓杨程韩冰吴昊张翀
Owner WUXI RES INST OF APPLIED TECH TSINGHUA UNIV
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