Culture device and culture method for double membrane aerating aerobiotic methane-oxidizing bacteria

Abstract

The invention discloses a culture device and a culture method for double membrane aerating aerobiotic methane-oxidizing bacteria. The method comprises the following steps: putting sludge rich in methane-oxidizing bacteria and a soil suspension into a reaction device cavity; and introducing methanol and oxygen by way of bubble-less aeration to achieve high-density and low-cost culture of the aerobiotic methane-oxidizing bacteria. The invention also provides the culture device for double membrane aerating aerobiotic methane-oxidizing bacteria. As solubility of methane and oxygen in water is relatively low, sufficient carbon sources and energy sources cannot be provided to microorganisms in a reaction device and methane mixed in air or oxygen exceeding a certain proportion has a risk of explosion, gas-liquid mass transfer resistance is broken through by way of double membrane aeration, so that the dissolved load of methane and oxygen in a solution is improved. A primer can be provided efficiently at low cost, and the culture device and culture method are suitable for culturing aerobiotic methane-oxidizing bacteria efficiently, and a high density aerobiotic methane-oxidizing bacteria culture is used for sewage treatment, intermediate product production, greenhouse gas emission reduction and the like.

Description

technical field [0001] The invention relates to a high-efficiency culture device and method for aerobic methane oxidizing bacteria, in particular to a double-membrane aerated aerobic methane Efficient cultivation of substrate-grown aerobic methanotrophs. Background technique [0002] The continuous emission of greenhouse gases leads to global warming, causing a series of environmental problems such as melting glaciers, rising sea levels and frequent occurrence of extreme weather, causing serious, widespread and irreversible impacts on human beings and ecosystems. Methane is the second largest greenhouse gas after carbon dioxide in the earth's atmosphere. Although the current concentration of methane in the atmosphere is far less than that of carbon dioxide, its greenhouse effect is that of CO 2 It contributes more than 18% to the intensity of atmospheric radiation, and has a more significant impact on climate change than carbon dioxide. [0003] Methanotrophs are special m...

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Problems solved by technology

[0006] The present invention is based on the relatively low solubility of methane and oxygen in water, which cannot provide sufficient carbon source and energy to the microorganisms in the reaction device, and the problem that there is a danger of explosion when methane is mixed in air or oxygen in excess of a certain proportion, and proposes a Novel aerobic methane oxidizing bacteria culture device and culture method

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