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Chryseobacterium sp. for removing phosphorus in sewage at low temperature and separation culture method

A technique for the isolation and cultivation of Chryseobacterium, applied in the direction of microorganism-based methods, isolated microorganisms, chemical instruments and methods, etc., can solve problems such as low-temperature dephosphorization effect is not good

Active Publication Date: 2012-05-02
GUOZHONG AIHUA TIANJIN MUNICIPAL ENCIRONMENT ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the deficiencies in the low-temperature dephosphorization effect of existing bacterial strains, and to provide a Chryseobacterium aureus that removes phosphorus in sewage

Method used

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  • Chryseobacterium sp. for removing phosphorus in sewage at low temperature and separation culture method
  • Chryseobacterium sp. for removing phosphorus in sewage at low temperature and separation culture method
  • Chryseobacterium sp. for removing phosphorus in sewage at low temperature and separation culture method

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Experimental program
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Effect test

Embodiment 1

[0024] The method for isolating and cultivating the Chryseobacterium aureus that removes phosphorus in sewage at low temperature comprises the following steps:

[0025] (1) Put the phosphorus-enriched culture solution into a small-scale SBR reactor, inoculate the sludge from the aeration tank of Tianjin Jizhuangzi Sewage Treatment Plant into the phosphorus-rich culture solution, and cultivate continuously for 20 days at 10°C. 6 hours, including anaerobic stirring for 2.5 hours, aerobic 2.5 hours, standing still for 0.5 hours, draining the supernatant and replenishing new phosphorus-rich culture solution for a total of 0.5 hours; keep the sludge concentration at 3000mg / L during operation;

[0026] (2) Take 10mL of the sludge obtained in step (1), add it to a 250ml Erlenmeyer flask filled with 90mL sterilized distilled water and 30 sterilized glass beads, and shake for 20min to fully release the bacterial cells into the supernatant; Stand still for 30min, take 1mL supernatant an...

Embodiment 2

[0030] Phosphorus-enriched culture fluid is composed of the following components:

[0031] CH 3 COONa·3H 2 O 3.32g, KH 2 PO 4 43.75mg, CaCl 2 2H 2 O 25.68mg, KNO 3 485.94mg, MgSO 4 ·7H 2 O 91.26mg, 5mL Tris buffer and 2mL trace elements; add distilled water to 1000mL, adjust the pH to 7.2;

[0032] The composition of described trace element solution is: EDTA 5g, FeSO 4 ·7H 2 O 5g, CuSO 4 ·5H 2 O 1.6g, MnCl 2 4H 2 O 5g, (NH 4 ) 6 Mo 7 o 2 4H2O 50mg, H 3 BO 3 50mg, KI 10mg and CoCl2 ·6H 2 Add distilled water to O 50mg to 1000mL.

Embodiment 3

[0034] Phosphorus-rich solid medium is composed of the following components:

[0035] CH 3 COONa·3H 2 O 3.32g, KH 2 PO 4 43.75mg, CaCl 2 2H 2 O 25.68mg, KNO 3 485.94mg, MgSO 4 ·7H 2 O 91.26mg, 5mL Tris buffer and 2mL trace elements, agar 20g, add distilled water to 1000mL, adjust the pH to 7.2;

[0036] The composition of described trace element solution is: EDTA 5g, FeSO 4 ·7H 2 O 5g, CuSO 4 ·5H 2 O 1.6g, MnCl 2 4H 2 O 5g, (NH 4 ) 6 Mo 7 o 2 4H 2 O 50mg, H 3 BO 3 50mg, KI10mg and CoCl 2 ·6H 2 Add distilled water to O 50mg to 1000mL.

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Abstract

The invention discloses Chryseobacterium sp. for removing phosphorus in sewage at low temperature and a separation culture method. The Chryseobacterium sp. deep yellow polyphosphorus CGMCC No.5282 for removing the phosphorus in the sewage at low temperature has the ability of removing the phosphorus in the sewage at low temperature under aerobic conditions. The experimental result shows that: in spite of obvious inhibition of a low-temperature environment, the Chryseobacterium sp. deep yellow polyphosphorus CGMCC No.5282 for removing the phosphorus in the sewage at low temperature has a remarkable effect of strengthening the removal of the phosphorus at low temperature, especially at the temperature of between 8 and 12 DEG C, and the removal rate of the phosphorus is improved by 10 percent; and the effluent standard is superior to the first-grade A effluent standard (water temperature is less than or equal to 12 DEG C, and the control index of the first-grade A standard is 0.5mg / L).

Description

technical field [0001] The invention belongs to the field of sewage treatment, and relates to a Chryseobacterium strain capable of removing phosphorus in sewage at low temperature and an isolation and cultivation method. Background technique [0002] The reason why low temperature has a significant impact on biological treatment capacity is that the decrease in temperature affects the growth and metabolism of microorganisms. Although phosphorus-accumulating bacteria are low-temperature psychrotrophic bacteria, the phosphorus-releasing and phosphorus-absorbing rates of phosphorus-accumulating bacteria are also affected during the temperature drop process, resulting in a decline in the quality of effluent from sewage treatment plants in winter. Therefore, there is an urgent need for a bacterial strain that can strengthen the removal of phosphorus in sewage under low temperature conditions. Contents of the invention [0003] The purpose of the present invention is to overcom...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C02F3/34C02F3/02C12R1/01C02F101/10
CPCY02W10/10
Inventor 胡春萍
Owner GUOZHONG AIHUA TIANJIN MUNICIPAL ENCIRONMENT ENG
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