Biological deferrization demanganization functional bacterium

A technology of functional bacteria and microbial strains, applied in the field of microorganisms, can solve the problems of long recovery period of engineering bacteria, weak biochemical ability, poor manganese removal effect, etc., and achieve the effect of shortening the establishment time, strong biochemical ability, and accelerated filtration speed

Inactive Publication Date: 2008-07-30
HARBIN INST OF TECH
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  • Abstract
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  • Claims
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Problems solved by technology

[0009] The purpose of the present invention is to solve the problem that the current biological oxidation manganese removal engineering bacteria group is established by natural selection, the biochem

Method used

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  • Biological deferrization demanganization functional bacterium
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  • Biological deferrization demanganization functional bacterium

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specific Embodiment approach 1

[0017] Specific embodiment 1: In this embodiment, the biological iron and manganese removal functional bacterium MSB-4 (Chryseobacterium sp.) belongs to the genus Chryseobacterium, and was preserved in the General Microbiology Center (CGMCC) of the China Microbiological Culture Collection Management Committee (CGMCC), and the preservation date was October 2007. On March 30th, the preservation number was CGMCC No.2239; biological iron and manganese removal functional bacteria MSB-4 (Chryseobacterium sp.) is an aerobic bacterium with a length of 1.5-2 μm and a width of 0.8-1.1 μm, short rod-shaped cells, Both ends are blunt and round (as shown in Figure 1 and Figure 2), and multiple bacteria aggregate or arrange in chains to form bacterial micelles with a common capsule; brownish-yellow colonies are formed on the culture medium, which are opaque and round shape, binary fission; biological iron and manganese removal functional bacteria MSB-4 (Chryseobacterium sp.) has energy stora...

specific Embodiment approach 2

[0020] Specific embodiment two: In this embodiment, the biological iron and manganese removal functional bacteria MSB-4 (Chryseobacterium sp.) is screened from the mud taken from the aeration tank wall of the biological manganese removal filter aeration tank of Zhangshi biological manganese removal water plant in Shenyang City, Liaoning Province owned. 1L medium for screening consists of 0.8g peptone, 0.2g yeast extract, 0.1g MnSO 4 .H 2 O, 0.1 g K 2 HPO 4 , 0.2g MgSO 4 ·7H 2 O, 0.2g NaNO 3 , 0.1g CaCl 2 , 0.1g (NH 4 ) 2 CO 3 , 0.01g FeSO 4 ·7H 2 O and the remainder of distilled water; then sterilized.

[0021] The present embodiment adopts the combination of plate streaking and plate coating method to divide bacteria, and adopts TMPD method (Vandenabeele J, D de Beer et al. Manganese oxidation by microbial consortia from sand filters. Microb. Ecol. 1992, 24: 91-108. ) for the primary screening of the isolated strains. The principle of using the TMPD method in th...

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Abstract

The invention provides a biological iron-removing manganese-removing functional bacterium, which relates to a microorganism. The invention solves the problems that the present biological oxidation manganese removing engineering flora is established by natural selection, the biochemical capability is weak, the manganese removing effect is poor; the establishment of the water treatment removing manganese biologic filter pond needs a long time for cultivation, domestication and startup, and the restoring period after the engineering flora is damaged is long. The biological iron-removing manganese-removing functional bacterium MSB-4(Chryseobacterium sp.) belongs to flavobacterium, is preserved in the General Microorganism Center of China Commission of Culture Collection Management, the storage date is 30 Octomber 2007, the storage number is CGMCC No.2239; strain MSB-4 is aerobic strain, the length is 1.5-2 micrometers, the width is 0.8-1.1 micrometers, the strain is short rod-shaped cell with two blunt ends; brown yellow colony is formed on the substrate, the colony is non-transparent and round; the strain MSB-4 has metachromatic granules and lipoid granules, has no brood cell; N, N, N<1>, N<1>-tetramethyl-para-phenylene diamine stain is positive. The biological iron-removing manganese-removing functional bacterium MSB-4 of the invention has the capability for removing the Mn<2+>, <Fe2+> in the water, Mn<2+> removing rate can reach up to 94.44 percent, and the Fe<2+> removing rate can reach up to 90.13 percent.

Description

technical field [0001] The present invention relates to a microorganism. Background technique [0002] The iron removal technology in water is relatively mature, and there are many engineering bacteria that can only remove iron. However, iron and manganese are abundant metal elements in the earth's crust, and the removal of iron alone is far from meeting the existing water treatment standards. The concentration of manganese ions in ordinary water is 0.1-1 mg / L, while the concentration of soluble manganese ions in anaerobic groundwater can be as high as 7-9 mg / L. Soluble manganese can form insoluble precipitates (such as MnO 2 or MnOOH). [0003] MnO 2 is a highly insoluble substance (δ-MnO 2 The solubility kso is only 10 -56 ), which is dark black and can cause significant coloration in groundwater intended for drinking; manganese must therefore be removed from drinking water. my country stipulates that the maximum concentration of manganese ions in drinking water is 0...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F1/64
Inventor 张杰赵焱李冬
Owner HARBIN INST OF TECH
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