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A new strain of natural yellow pigment producing bacteria xj2 and its preparation and application

A yellow pigment, XJ2 technology, applied in the field of microbial engineering, can solve the problem that the type and quantity of strains cannot meet the needs of natural yellow pigment research and development, etc.

Active Publication Date: 2020-02-21
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the current state of the art, especially the types and quantities of strains that are far from meeting the technical problems of various industries for the research and development of natural yellow pigments, this application provides a yellow strain isolated from chickpea root nodules in Mulei, Xinjiang. A typical new strain, the natural yellow pigment prepared by this strain has wide application value in the production and research of natural yellow pigment

Method used

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  • A new strain of natural yellow pigment producing bacteria xj2 and its preparation and application
  • A new strain of natural yellow pigment producing bacteria xj2 and its preparation and application
  • A new strain of natural yellow pigment producing bacteria xj2 and its preparation and application

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Embodiment 1

[0030]Example 1: Screening, classification and identification of Chryseobacterium sp. XJ2 CGMCC No.13157

[0031] Chryseobacterium sp. XJ2 CGMCC No. 13157 used in the present invention is isolated from Chickpea root nodules in Mulei, Xinjiang. Rinse the soil on the surface of the chickpea root with tap water, rinse the root nodules with sterile water for more than 5 times, soak the root nodules with 95% ethanol for 30 seconds, continue to rinse with sterile water for more than 5 times, and soak them in 0.1% mercuric chloride solution for 5 times. After 10 minutes, rinse the root nodules with sterile water for more than 5 times, and keep the sterile water from the last rinse and spread it on the relevant plate to detect whether the root nodules are thoroughly disinfected. Then use a scalpel to peel off the root nodules from the chickpea roots, grind the sterilized nodules with a sterile mortar, and after appropriate dilution, use the tip of a gun to draw 0.1mL of the diluted so...

Embodiment 2

[0038] Embodiment 2: Chryseobacterium sp. (Chryseobacterium sp.) XJ2 CGMCC No.13157 Fermentation produces and extracts the method and light-absorbing characteristic of yellow pigment

[0039] In the present invention, Chryseobacterium sp. (Chryseobacterium sp.) XJ2 CGMCC No.13157 is inoculated in the fermentation medium (500mL Erlenmeyer flask filling 100mL fermentation medium, fermentation medium peptone 10g, yeast extract 5g, glucose 1g, KH 2 PO 4 3 g, pH7.0), 30 ° C, 200 rpm shaker culture for 3 days. The bacterial liquid was centrifuged at 8000rpm for 3min to obtain the bacterial cells. After the bacterial cells were washed with normal saline three times, 20mL of 95% ethanol solution was added, 50°C, 100rpm for oscillating extraction for 1h; then centrifuged at 8000rpm for 5min, the obtained supernatant The liquid is the crude yellow pigment extract; the crude yellow pigment extract is placed at a constant temperature of 60°C for vacuum concentration until it becomes dry ...

Embodiment 3

[0041] Example 3: Determination of Solubility of Yellow Pigment Produced by Chryseobacterium sp. XJ2 CGMCC No.13157

[0042] Chryseobacterium sp. (Chryseobacterium sp.) XJ2 CGMCC No.13157 was fermented and the crude extract of yellow pigment was obtained, and 10 mL of purified water, 50% ethanol, 95% ethanol, 99.8% ethanol, 50% methanol solution, Put 100% methanol, ethyl acetate, acetone, and n-butanol in colorimetric tubes, and add absolute excess yellow pigment crude extracts in various solvents. After sealing, shake and dissolve in a shaker at room temperature at 100 rpm for 12 hours to prepare saturated yellow pigment. Pigment solution. Then centrifuge at 8000rpm for 5min, take the supernatant to measure the absorbance at a wavelength of 438nm, compare it with the OD of 95% ethanol saturated yellow pigment solution, and calculate the dissolution rate, see Table 2.

[0043] Table 2: Effect of different solutions on extraction

[0044]

[0045] As shown in Table 2, it c...

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Abstract

The invention discloses a new strain of natural yellow pigment producing bacteria XJ2 and its pigment preparation. Chryseobacterium sp. ) The identification of XJ2 CGMCC No.13157 shows that it belongs to a typical new strain. It produces natural yellow pigment through activation, proliferation and extraction. The natural yellow pigment produced is soluble in water, methanol and ethanol, and easily soluble in acetone and acetic acid. Low polar solvents such as ethyl ester and n-butanol, the ethanol solution of the extract has a maximum absorption peak at 438nm; it has anti-oxidation function; it is stable in the range of pH2.0~9.0; it has good thermal stability and certain light Stability has great practical significance for the production of natural yellow pigment.

Description

technical field [0001] The invention mainly relates to the technical field of microbial engineering, in particular, the invention relates to a production strain of a natural pigment, a pigment and a preparation method. Background technique [0002] Pigment refers to the substance that can color the mordant, also called coloring agent, which can be used to change the color of articles or food, and is widely used in textiles, construction, food, biology, medicine and health, cosmetics, daily industrial products, feed, etc. In the field of industry, pigments are divided into two categories: natural pigments and synthetic pigments according to their sources. [0003] Natural pigments are mainly natural pigments extracted from plant roots, stems, leaves, flowers, fruits, animals, and microorganisms. They are pigments that people have been using long before the emergence of synthetic pigments. Compared with synthetic pigments, most natural pigments have no toxic and side effects,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04C12R1/01
CPCC12P1/04C12N1/205C12R2001/01
Inventor 高雁艾尼江·尔斯满楚敏张志东霍向东史应武杨红梅林青
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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