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Bacillus aureus, inoculants containing the same, applications thereof, and methods for degrading plastics

A technology of Chryseobacterium aureus and degrading plastics, applied in the field of microorganisms, can solve the problems of high cost, less biodegradation of plastic products, and harsh degradation conditions, and achieve the effects of low cost, low production cost and convenient operation

Active Publication Date: 2022-07-29
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, countries all over the world are actively exploring plastic degradation technology. Traditional physical and chemical degradation methods, such as acidolysis, alkali hydrolysis, alcoholysis, pyrolysis, etc., often have problems such as harsh degradation conditions and high costs. Therefore, new biodegradation methods Plastic technology has become a hotspot in current research and has been included in a number of key support programs
However, there are few reports on the biodegradation of plastic products

Method used

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  • Bacillus aureus, inoculants containing the same, applications thereof, and methods for degrading plastics
  • Bacillus aureus, inoculants containing the same, applications thereof, and methods for degrading plastics
  • Bacillus aureus, inoculants containing the same, applications thereof, and methods for degrading plastics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] (1) Isolation of strains

[0064] Take the waste from a landfill in Nanjing as the sample to be separated for separation and screening: Weigh 1g of the collected landfill waste sample and place it in 100mL containing diethyl terephthalate (polyethylene terephthalate). The structural analog of alcohol ester, the concentration is 2g / L) in the inorganic salt liquid medium (diethyl terephthalate is the only carbon source), in a constant temperature shaking incubator at a temperature of 30 ° C and a speed of 150 rpm The next 4-6 days of shaking culture is one round. After five rounds of shaking culture, take the culture solution of the fifth round to prepare a bacterial suspension, and dilute the bacterial suspension to 10 by volume gradient. -6 , 10 -7 , 10 -8 times the diluted bacterial suspension; draw 0.1 mL gradient of the diluted bacterial suspension and spread it on the inorganic salt solid medium containing high-temperature sterilized polyethylene terephthalate par...

Embodiment 2

[0075] (1) The Bacillus aureus PET-29 obtained in Example 1 was cultured in a LB liquid medium at a temperature of 30° C. and a rotational speed of 150 rpm to a logarithmic phase as a first-class seed solution, and the first-class seed solution was divided by volume. 1.5% of the inoculum was inoculated into the LB liquid medium, and the temperature was 30 ° C and the rotation speed was 150 rpm under the conditions of shaking culture to logarithmic phase to obtain secondary seed liquid;

[0076] (2) The secondary seed solution was inoculated into 50 mL of LB liquid medium at a volume ratio of 1% at a temperature of 30 °C, a rotation speed of 150 rpm, and pH of 5.5, 6.0, 6.5, 7.0, 7.5, and 8.0, respectively. Cultivated for 60h under conditions, and sampled at 6h, 12h, 24h, 36h, 48h, and 60h; dilute the sample of the fermentation broth by 10 times, and detect the OD 600 , measured the growth curve of Bacillus aureus PET-29, the results are as follows Figure 4 shown.

[0077] f...

Embodiment 3

[0079] (1) The Bacillus aureus PET-29 obtained in Example 1 was cultured in a LB liquid medium at a temperature of 30° C. and a rotational speed of 150 rpm to a logarithmic phase as a first-class seed solution, and the first-class seed solution was divided by volume. 1.5% of the inoculum was inoculated into the LB liquid medium, and the temperature was 30 ° C and the rotation speed was 150 rpm under the conditions of shaking culture to logarithmic phase to obtain secondary seed liquid;

[0080] (2) The secondary seed solution was inoculated into 50 mL of LB liquid medium at a volume ratio of 1%, at a temperature of 20 °C, 30 °C, 37 °C, and 45 °C, a rotation speed of 150 rpm, and a pH of 6. Culture for 60h, take samples at 6h, 12h, 24h, 36h, 48h, and 60h; dilute the sample of the fermentation broth by 10 times, and detect OD 600 , measured the growth curve of Bacillus aureus PET-29, the results are as follows Figure 5 shown.

[0081] from Figure 5 It can be seen that the g...

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Abstract

The invention relates to the field of microorganisms, and discloses a strain of aureus aureus, a bacterial agent containing the bacteria, applications thereof and a method for degrading plastics. The bacterial strain of the aureobacter aureus is named as the aureus PET-29 ( Chryseobacterium sp. PET‑29), the deposit number is CCTCC NO:M 2022311. The microbial inoculum provided by the present invention contains the aforementioned Bacillus aureus. The present invention also provides the application of the above-mentioned Bacillus aureus and the above-mentioned bacterial agent in degrading plastics. The method for degrading plastic comprises: contacting the above-mentioned aureobacteria and / or the above-mentioned bacterial agent with plastic. The Bacillus aureus strain provided by the invention or the bacterial agent containing the strain can be used for biodegrading polyethylene terephthalate plastics, in the discovery and transformation of new plastic degrading enzymes, the recycling of waste plastics, the bioremediation of environmental plastic pollution, etc. The field has the prospect of further research and development and engineering application.

Description

technical field [0001] The present invention relates to the field of microorganisms, in particular, to a strain of aureus aureus, a bacterial agent containing the bacteria, their application and a method for degrading plastics. Background technique [0002] With the rapid economic development, plastics are widely used in construction, machinery, agriculture, food packaging and other fields because of their waterproof, cheap, convenient and other characteristics, involving all aspects of human life. The global annual consumption of plastic exceeds 300 million tons and is increasing year by year. Plastic products are not only difficult to degrade, but also have low recycling rate. Their continuous accumulation in the environment poses a serious threat to the ecology. Plastic waste pollution has become one of the most concerned environmental problems in the world, and about 60% of plastic waste is For polyethylene terephthalate (Polyethylene terephthalate, PET) plastic product...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C08J11/10C08L67/02C12R1/01
CPCC12N1/20C08J11/10C08J2367/02Y02W30/62
Inventor 黄和李秀娟李安妮盛义杰王心悦张雨萱叶盛雪张立慧徐晴
Owner NANJING NORMAL UNIVERSITY
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