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Method for preparing rubusoside by stevioside

A technology of steviol glycoside and rubusoside, applied in the field of food additive application, can solve the problems of insufficient raw materials, high price, low product purity, etc., achieve the effect of increasing added value and solving the effect of limited source of raw materials

Inactive Publication Date: 2011-11-16
NANJING NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method can solve the problems of insufficient raw materials for rubusoside preparation, low product purity and high price

Method used

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  • Method for preparing rubusoside by stevioside
  • Method for preparing rubusoside by stevioside
  • Method for preparing rubusoside by stevioside

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Prepare 1% (W / W), pH 7.5 stevioside (SS accounted for 72%, RA accounted for 25%) transformation substrate, insert 10% (V / V) JH bacterial solution, 30°C, 200r / min dynamic culture 3 sky. After the cultivation, the bacteria were removed by centrifugation at 5000rpm. The supernatant was flocculated and precipitated to remove impurities, the macroporous resin was adsorbed and separated, and the eluent was crystallized and purified to obtain high-purity rubusoside. The purity was about 95%, and the conversion rate of stevioside was 90%. %.

Embodiment 2

[0044] Put 0.01% (W / W), pH 3.5 stevioside substrate in a 500mL Erlenmeyer flask, add 0.01% (W / W) bacterial powder, and culture at 30°C for 1 day. Separation and purification to obtain high-purity rubusoside.

Embodiment 3

[0046] 0.05% (W / W), pH 5.5 stevioside substrate in a 500mL transformation bottle, add 1% (W / W) bacterial powder, and incubate at 30°C for 12h. After separation and purification, high-purity rubusoside is obtained.

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Abstract

The present invention relates to a preparation raw material and a preparation method of rubusoside, which belongs to the field of food additives. Stevioside is transformed into rubusoside by the catalysis of chryseobacterium JH (Chryseobacterium sp.JH) CCTCC NO: M209202 or beta-glucosidase produced by the same. Concretely, the stevioside is prepared into bacterium culture liquid or enzyme transformation liquid and is transformed into rubusoside under the conditions of proper liquid loading quantity, proper transformation temperature, proper pH, proper time and the like after a right amount of bacteria or enzymes are added, and the rubusoside with high purity is obtained after the transformed rough transformation liquid is further separated and purified. The invention provides a novel raw material for preparing the rubusoside, which expands the source of raw materials and has stronger practical value.

Description

technical field [0001] The invention relates to a new raw material and a new method for preparing rubusoside, especially a method for obtaining rubusoside by transforming steviol glycoside with microorganisms or enzymes. It belongs to the application field of food additives. Background technique [0002] Sweet tea (Rubus suavissimus) is a perennial deciduous shrub with thorns in the genus Rubus of the family Rosaceae. It is mainly produced in Liuzhou, Guilin, Wuzhou and other regions in Guangxi. It is a unique wild and rare sweet plant in Guangxi. A large sweet plant whose leaves can be used as medicine and tea (Chen Quanbin, Shen Zhongsu, Zhang Qiaoyun, etc. Isolation, purification and characterization of flavonoid aglycones in sweet tea. Forestry Science and Technology. 2005, 30(1): 45-48.). [0003] The main sweet component of sweet tea leaves is Rubusoside (RS for short), which accounts for about 2.0% of the dry leaf weight of sweet tea leaves. Tetracyclic diterpene gl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/56C12R1/01
Inventor 陈育如姜中玉刘虎
Owner NANJING NORMAL UNIVERSITY
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