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Indole-producing Chryseobacterium sp. And application thereof

The technology of aureobacter aureus indole and aureobacter aureus is applied in the field of microorganisms, which can solve the problems of imperfect use development of the aureobacter aureus indole, the utilization rate of harmful plant source control agents, etc., and achieve the effect of great application potential.

Active Publication Date: 2022-07-05
沈阳恩柽研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] In view of the harm to the environment of current nematode chemical control agents and the low actual use rate of plant source control agents, in order to solve the problem of finding new microbial strains for nematode control and the development of the use of Chryseobacterium indole-producing in the prior art is not perfect problem, the present invention provides a Chrysobacterium indole-producing strain and its application, said Chryseobacterium indole-producing strain is isolated from M-type pine xylophilus for the preparation of antibacterial products and / or anti-nematode products

Method used

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  • Indole-producing Chryseobacterium sp. And application thereof
  • Indole-producing Chryseobacterium sp. And application thereof
  • Indole-producing Chryseobacterium sp. And application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1 Isolation and identification of EC200816 bacteria

[0077] 1. Isolation and culture of EC200816 bacteria

[0078] (1) The source of B. xylophilus tested and the expanded culture of B. xylophilus

[0079] Source: M-type pine wood nematodes subcultured in the laboratory for a long time;

[0080] Cultivation: Weigh 300g of dry corn kernels with complete shape and soak them in water for 2 days at room temperature, and then pack them into 10 conical flasks with a capacity of 250mL. Sterilize at °C for 30 minutes. After the sterilization is completed, let it naturally cool to room temperature, inoculate an appropriate amount of Botrytis cinerea under aseptic conditions, and cultivate in a 25°C, 70% RH artificial climate box for 5-7 days in the dark; Inoculate 3,000-5,000 pine wood nematodes in the bottle, and continue culturing for 8-15 days in a 25°C, 70% RH artificial climate box in the dark, and then a large number of M-type pine wood nematodes can be harveste...

Embodiment 2

[0107] Example 2 Drug susceptibility test of EC200816 bacteria

[0108] 1. Medication

[0109] Streptomycin (STR): use streptomycin sulfate to prepare a stock solution of 1280 μg / mL, and use the double dilution method to gradually dilute to 640, 320, and 160 μg / mL of liquid.

[0110] Gentamicin (GEN): use gentamicin sulfate to prepare a 1280 μg / mL stock solution, and use the double dilution method to dilute into 640, 320, and 160 μg / mL liquids step by step.

[0111] 2. Drug sensitivity test

[0112] MHA (beef powder 6g / L, soluble starch 1.5g / L, acid hydrolyzed casein 17.5g / L, agar 17g / L) agar plate medium was prepared, and the drug sensitivity test was detected by disc diffusion method.

[0113] Treatment of drug sensitive paper: Take sterile blank drug sensitive paper and place it on a sterile and clean petri dish, and drop 20uL of each drug to be tested on the drug sensitive paper, so that the drug content of the drug sensitive paper is 25.6, 12.8 and 6.4μg / mL were natura...

Embodiment 3

[0120] Example 3 Experiments on the anti-pinewood nematode activity of the whole cell culture broth and fermentation broth of EC200816 bacteria

[0121] 1. Experimental materials

[0122] Test insect source: R-type pine wood nematode cultured in the laboratory for a long time;

[0123] Cultivation: Weigh 300g of dry corn kernels with complete shape and soak them in water for 2 days at room temperature, and then pack them into 10 conical flasks with a capacity of 250mL. Sterilize at °C for 30 minutes. After the sterilization is completed, let it cool down to room temperature naturally, inoculate an appropriate amount of Botrytis cinerea under aseptic conditions, and incubate it in a 25°C, 70% RH artificial climate box for 5-7 days in the dark and then put it into each conical flask. Inoculate 3,000-5,000 R-type pine wood nematodes, and continue to cultivate for 8-15 days in a 25°C, 70% RH artificial climate box in the dark, and then a large number of R-type pine wood nematode...

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Abstract

The invention provides a Chryseobacterium indologenes producing strain, a culture of the strain, an application of the strain in resisting nematodes, and a use method of the strain and the culture. The culture (including fermentation broth, metabolite and extract) of the Chryseobacterium indole and the compound separated from the culture can inhibit the development of various nematodes, especially has a remarkable inhibition effect on pine wood nematodes, can be used for preparing corresponding products capable of being practically applied, and has wide application prospects. The application potential in the fields of medicines, pesticides and cleaning products is huge.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an indole-producing Bacillus aureus and its application. Background technique [0002] Natural products refer to the collective term for the constituents, metabolites and / or endogenous chemical constituents in animals, plants, insects, marine organisms and microorganisms. Microbial natural products provide a rich source of chemical diversity, and are one of the important sources of medicinal small molecules, food additives, and anti-insect and antibacterial agents. The above-mentioned chemical substances with important functions are usually derived from secondary metabolites of microorganisms, which can be used alone or in combination with known compounds to achieve the desired purpose of anti-insect, antibacterial and the like. [0003] Nematodes, belonging to the phylum Nematoda, can be seen everywhere in freshwater, seawater, and land. They are an animal group second o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01N51/00A01N43/90A01P5/00A01P7/04A61K35/74A61K8/99A61Q17/00A61P33/10C12R1/01
CPCC12N1/20A01N63/20A01N51/00A01N43/90A61K35/74A61K8/99A61Q17/00A61P33/10Y02A50/30
Inventor 李世友赵福强严弋敬
Owner 沈阳恩柽研究院有限公司
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