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A strain of Chryseobacterium efficiently degrading picolinic acid and its application

A technology of picolinic acid and Bacillus aureus, applied in the field of bioengineering, can solve the problems of bacterial activity inhibition, inhibition, inability to effectively remove picolinic acid, etc., to achieve the effect of enhancing processing capacity and broad application potential

Active Publication Date: 2019-05-03
XI AN JIAOTONG UNIV
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Problems solved by technology

Under strong acidic conditions, the growth of picolinic acid-degrading bacteria reported in the literature is significantly inhibited, so picolinic acid cannot be effectively removed
In addition, the picolinic acid-degrading bacteria reported in the literature usually show high activity between 25 and 30°C, while the activity of the strains is significantly inhibited under low temperature conditions, that is, when the temperature is ≤20°C
Therefore, the picolinic acid degrading bacteria reported in the literature are difficult to be used in low temperature environment

Method used

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  • A strain of Chryseobacterium efficiently degrading picolinic acid and its application
  • A strain of Chryseobacterium efficiently degrading picolinic acid and its application
  • A strain of Chryseobacterium efficiently degrading picolinic acid and its application

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Embodiment Construction

[0026] The present invention will be further described in detail below in conjunction with the technical scheme and the accompanying drawings.

[0027] 1, the chryseobacterium provided by the invention, its screening step is as follows:

[0028] Aerobic activated sludge samples were collected from the aeration tank of a pharmaceutical factory in Xi'an, and used as a source of bacteria for domestication and cultivation. Add 0.2% sodium pyrophosphate to 50mL of activated sludge as a deflocculant, shake on the shaker for 5-10min to break up the activated sludge flocs, take 5mL of activated sludge, add 95mL of inorganic salt medium to shake In the bottle, add picolinic acid as the only carbon, nitrogen, and energy source, wherein the concentration of picolinic acid is 100 mg / L, and culture on a shaking table at a temperature of 30° C. and a rotation speed of 180 r / min. Inorganic salt medium composition: Na 2 HPO 4 12H 2 O, 7g / L; KH 2 PO 4 , 1g / L; CaCl 2 2H 2 O, 10mg / L; FeC...

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Abstract

The invention provides a strain of Chryseobacterium that efficiently degrades picolinic acid and its application, belonging to the technical field of bioengineering. The present invention isolates and screens a picolinic acid-degrading bacterium, Chryseobacterium sp., which is classified as Chryseobacterium sp. and is preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, with the preservation number CGMCC NO.10699. The advantage of the present invention is that this Chryseobacterium can effectively degrade picolinic acid under the condition that the pH value range is wider or the temperature range is wider, and the pH value range is wider and the temperature range is wider; The aerobic biological treatment of picolinic acid wastewater in a wide temperature range from weak base to weak acid has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a strain of Chryseobacterium efficiently degrading picolinic acid and its application. Background technique [0002] Picolinic acid and its derivatives are widely used in the production of medicines, pesticides and daily chemicals, and the widespread use of picolinic acid leads to environmental pollution. Picolinic acid wastewater can be treated by aerobic biological method. At present, the microbial resources for aerobic degradation of picolinic acid mainly include Arthrobacter, Mycobacterium and Streptomyces. There are few reports on strains of other genera. [0003] The most important use of picolinic acid in industry is the production of medicines and pesticides. The resulting wastewater has a high acidity and a pH value between 4 and 6. Under strong acidic conditions, the growth of picolinic acid-degrading bacteria reported in the literature is signific...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC02F3/34C02F2101/38C12N1/205C12R2001/01
Inventor 郑春莉沈振兴何炽刘萍萍刘红霞冯珊珊王巧蕊
Owner XI AN JIAOTONG UNIV
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