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Chryseobacterium sp. L31 and application thereof

A technology of Chrysobacterium aureus and strains, applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems that the degradation efficiency of cypermethrin-degrading bacteria is not very high, and there are few reports on the surface characteristics of degrading bacteria cells

Inactive Publication Date: 2011-01-19
中国农业科学院研究生院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, there are very few types of cypermethrin-degrading bacteria that have been screened at home and abroad. The degradation efficiency of the published cypermethrin-degrading bacteria is not very high, and there are few reports on the cell surface characteristics of the degrading bacteria, mainly focusing on the degradation of petroleum hydrocarbons. research on bacteria

Method used

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  • Chryseobacterium sp. L31 and application thereof
  • Chryseobacterium sp. L31 and application thereof
  • Chryseobacterium sp. L31 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 bacterial strain screening method

[0028] 1) Enrichment and domestication of degrading bacteria

[0029] Inoculate 1 g of the activated sludge sample collected from the sewage treatment tank of Shandong Huayang Pesticide Factory into the enrichment medium containing cypermethrin 50mg / L, enrich and culture at 30°C 200rpm for 7 days, then transfer 10% to the fresh enrichment medium In the culture medium, the pesticide concentration was doubled, and the culture was continued for 7 days, so that the transfer was continued for 4 times until the pesticide concentration in the enriched medium was increased to 800mg / L. After the enrichment culture, 10% of the cells were collected by centrifugation, then transferred to an inorganic salt medium containing 800 mg / L cypermethrin, and acclimatized for another two weeks under the same culture conditions.

[0030] The composition of enrichment medium TYC is as follows: tryptone 5g / L, yeast extract 5g / L, KH 2 PO 4 1g / L...

Embodiment 2

[0034] The degradation performance of embodiment 2 degrading bacteria

[0035] 1) HPLC detection of cypermethrin degradation

[0036] The bacterial strain L31 was inoculated into liquid LB medium without pesticides for activation, cultivated to the logarithmic growth phase, inoculated into the basic salt medium containing 50 mg / L cypermethrin pesticide according to 20% inoculum amount, and cultured on a shaking table at 30° C. at 200 rpm.

[0037] The composition of liquid LB medium is: tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L, pH7.0.

[0038] Basal salt medium contains the following components: NH 4 NO 3 1g / L, MgSO 4 ·7H 2 O 0.5g / L, (NH 4 ) 2 SO 4 0.5g / L, KH 2 PO 4 0.5g / L, NaCl 0.5g / L, K 2 HPO 4 1.5g / L, pH7.0.

[0039] The extraction method of high chlorine in the culture medium:

[0040] After regular sampling, add an equal volume of dichloromethane, fully oscillate on a vortex mixer to extract for 2 minutes, let it stand for one hour, remove the lower l...

Embodiment 3

[0047] Example 3 Determination of Cypermethrin Degrading Strain L31 Cell Surface Hydrophobicity

[0048] The present invention uses the MATH method to measure the cell surface hydrophobicity of the efficient-cypermethrin-degrading strain L31 (Rosenberg et al., 1980).

[0049] 1) Preparation of bacterial suspension

[0050] The cypermethrin-degrading bacteria L31 was inoculated in LB liquid medium (the composition is the same as in Example 2), cultured overnight at 30°C and 200rpm constant temperature shaking culture conditions, and the culture solution was centrifuged at 6,000g at 4°C for 10min to collect the bacteria and washed with 50mM Na 2 HPO 4 -NaH 2 PO 4 Wash twice with a buffer solution (pH 7.0), and then use the same buffer solution to suspend the bacteria until the cell concentration is about OD600=0.6 to prepare a bacteria suspension.

[0051] 2) Selection of two-phase separation system

[0052] Add 4mL of the bacterial suspension whose concentration has been a...

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Abstract

The invention provides a Chryseobacterium sp. L31 strain of which the preservation number is CGMCC No. 4137, which is separated and obtained from a sewage treatment aeration tank of Shandong Huayang pesticide factory by an acclimating method. The strain can degrade cypermethrin pesticide of 50mg / L by about 71% in 3 days, and can maintain stable degradation ability. In addition, the cell surface of the strain has very high hydrophobicity, and the hydrophobicity of the cell surface can be adjusted for finishing adsorption degradation of the hydrophobic pesticide.

Description

technical field [0001] The invention relates to a microbial strain and its application, in particular to a Chrysobacterium aureus L31 and its application. Background technique [0002] At present, there are very few types of cypermethrin-degrading bacteria that have been screened at home and abroad. The degradation efficiency of the published cypermethrin-degrading bacteria is not very high, and there are few reports on the cell surface characteristics of the degrading bacteria, mainly focusing on the degradation of petroleum hydrocarbons. in the study of bacteria. Tallur et al. isolated a strain of Micrococcus sp. CPN 1 (Micrococcus sp.CPN 1) in 2008 through the method of enrichment and domestication, which can degrade 100 mg / L of cypermethrin by about 55% within 3 days (Biodegradation, 19: 77-82 , 2008); Chen Zhang et al. isolated two Serratia strains (Bioresource Technology 101(2010) 3423-3429) that degraded beta-cypermethrin by enrichment and domestication in 2010, and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/04A62D101/28
Inventor 闫艳春曲杰史延华李康翟逸王圣惠
Owner 中国农业科学院研究生院
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