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Preparation method of microbial flocculating agent for treating lead-zinc waste water

A microbial flocculant, lead-zinc technology, applied in the field of wastewater treatment, can solve the problems of secondary pollution, strong corrosion, high cost, etc., and achieve the effect of good treatment effect, no secondary pollution, and simple process

Inactive Publication Date: 2018-10-16
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, aluminum salt, iron salt and other inorganic flocculants have the advantages of good treatment effect and low cost, but they are easy to cause secondary pollution and produce sludge containing a large amount of aluminum and iron, which is difficult to treat.
In addition, aluminum ions in aluminum salts have a certain relationship with the onset of senile dementia; iron salt flocculants not only make the effluent color, but also have strong corrosiveness; therefore, inorganic flocculants are not conducive to long-term use
The cost of organic polymer flocculants such as polyacrylamide is relatively high, and it is difficult to degrade by itself in the natural environment, causing secondary pollution, so the use of organic flocculants is also restricted to a certain extent

Method used

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  • Preparation method of microbial flocculating agent for treating lead-zinc waste water
  • Preparation method of microbial flocculating agent for treating lead-zinc waste water
  • Preparation method of microbial flocculating agent for treating lead-zinc waste water

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Embodiment

[0022] 1. Preparation of Chryseobacterium sp. ZW5 microbial flocculant

[0023] (1) Separation medium:

[0024] Beef extract peptone medium: 3.0 g beef extract, 10.0 g peptone, 5.0 g sodium chloride, 20.0 g agar, 1 L distilled water, pH =7.0;

[0025] LB (Luria-Bertani) medium: 10.0 g peptone, 5.0 g yeast extract, 6 g sodium chloride, 20.0 g agar, 1 L distilled water, pH = 7.0.

[0026] (2) Fermentation medium: 20.0 g glucose, 2.0 g potassium dihydrogen phosphate, 5.0 g dipotassium hydrogen phosphate, 0.2 g ammonium sulfate, 0.1 g sodium chloride, 0.5 g urea, 0.5 g yeast extract, 0.2 g magnesium sulfate Mix with 1 L of distilled water and adjust the pH of the resulting solution to 7.0.

[0027] (3) Seed medium: Mix 3.0 g of beef extract, 5.0 g of peptone, 5.0 g of sodium chloride and 1 L of distilled water, and adjust the pH of the resulting solution to 7.0.

[0028] (4) Bacterial source preparation: The soil was collected from the lead-zinc-contaminated soil in Sidi Villag...

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Abstract

The invention discloses a preparation method of a microbial flocculating agent for treating lead-zinc waste water. The preparation method comprises the steps of (1) separating out a strain Chryseobacterium sp with flocculating ability from soil polluted by lead and zinc; (2) inoculating a single strain in a seed medium to obtain a seed culture solution; (3) inoculating the seed culture solution obtained from step (2) in a fermenting medium, and culturing to obtain a fermentation culture solution; and (4) centrifuging the fermentation culture solution obtained from step (3) to obtain supernateor a supernate extractor which is the microbial flocculating agent. The preparation method is simple in process and low in cost, and the prepared flocculating agent has good effect of treating the lead-zinc waste water, and does not cause secondary pollution.

Description

technical field [0001] The invention belongs to the technical field of wastewater treatment, and relates to a method for treating lead-zinc wastewater, in particular to a method for preparing a microbial flocculant for treating lead-zinc wastewater. Background technique [0002] my country is rich in non-ferrous metal resources, among which lead and zinc mines rank third in the world. Lead and zinc are used in electrical, petroleum, chemical and other industries. They are important strategic resources that pollute the environment and affect people's health. For example, the accumulation of lead in the body of humans and animals not only affects the health of the body but can even be fatal; excessive intake of zinc can cause gastrointestinal discomfort, and seriously damage the liver function to a certain extent. [0003] The flocculation method is widely used in the wastewater treatment of lead-zinc metal flotation beneficiation. Among them, aluminum salt, iron salt and oth...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12P1/04C02F101/20C12R1/01
CPCC02F3/34C02F2101/20C12P1/04C12N1/205C12R2001/01
Inventor 陆燕勤覃敏杰刘燊
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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