Preparation method of microbial flocculating agent for treating lead-zinc waste water
A microbial flocculant, lead-zinc technology, applied in the field of wastewater treatment, can solve the problems of secondary pollution, strong corrosion, high cost, etc., and achieve the effect of good treatment effect, no secondary pollution, and simple process
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[0022] 1. Preparation of Chryseobacterium sp. ZW5 microbial flocculant
[0023] (1) Separation medium:
[0024] Beef extract peptone medium: 3.0 g beef extract, 10.0 g peptone, 5.0 g sodium chloride, 20.0 g agar, 1 L distilled water, pH =7.0;
[0025] LB (Luria-Bertani) medium: 10.0 g peptone, 5.0 g yeast extract, 6 g sodium chloride, 20.0 g agar, 1 L distilled water, pH = 7.0.
[0026] (2) Fermentation medium: 20.0 g glucose, 2.0 g potassium dihydrogen phosphate, 5.0 g dipotassium hydrogen phosphate, 0.2 g ammonium sulfate, 0.1 g sodium chloride, 0.5 g urea, 0.5 g yeast extract, 0.2 g magnesium sulfate Mix with 1 L of distilled water and adjust the pH of the resulting solution to 7.0.
[0027] (3) Seed medium: Mix 3.0 g of beef extract, 5.0 g of peptone, 5.0 g of sodium chloride and 1 L of distilled water, and adjust the pH of the resulting solution to 7.0.
[0028] (4) Bacterial source preparation: The soil was collected from the lead-zinc-contaminated soil in Sidi Villag...
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