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Chryseobacterium for efficient degradation of picolinic acid, and applications thereof

A picolinic acid and aureobacillus technology, applied in the field of bioengineering, can solve the problems of bacterial activity inhibition, inhibition, inability to effectively remove picolinic acid, etc., to achieve the effect of enhancing processing capacity and broad application potential

Active Publication Date: 2016-11-30
XI AN JIAOTONG UNIV
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Problems solved by technology

Under strong acidic conditions, the growth of picolinic acid-degrading bacteria reported in the literature is significantly inhibited, so picolinic acid cannot be effectively removed
In addition, the picolinic acid-degrading bacteria reported in the literature usually show high activity between 25 and 30°C, while the activity of the strains is significantly inhibited under low temperature conditions, that is, when the temperature is ≤20°C
Therefore, the picolinic acid degrading bacteria reported in the literature are difficult to be used in low temperature environment

Method used

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  • Chryseobacterium for efficient degradation of picolinic acid, and applications thereof
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  • Chryseobacterium for efficient degradation of picolinic acid, and applications thereof

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Embodiment Construction

[0026] The present invention will be further described in detail below in conjunction with the technical scheme and the accompanying drawings.

[0027] 1, the chryseobacterium provided by the invention, its screening step is as follows:

[0028] Aerobic activated sludge samples were collected from the aeration tank of a pharmaceutical factory in Xi'an, and used as a source of bacteria for domestication and cultivation. Add 0.2% sodium pyrophosphate to 50mL of activated sludge as a deflocculant, shake on the shaker for 5-10min to break up the activated sludge flocs, take 5mL of activated sludge, add 95mL of inorganic salt medium to shake In the bottle, add picolinic acid as the only carbon, nitrogen, and energy source, wherein the concentration of picolinic acid is 100 mg / L, and culture on a shaking table at a temperature of 30° C. and a rotation speed of 180 r / min. Inorganic salt medium composition: Na 2 HPO 4 12H 2 O, 7g / L; KH 2 PO 4 , 1g / L; CaCl 2 2H 2 O, 10mg / L; FeC...

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Abstract

The invention provides a strain of Chryseobacterium for efficient degradation of picolinic acid, and applications thereof, and belongs to the technical field of biological engineering. According to the present invention, a strain of picolinic acid degradation bacterial Chryseobacterium is separated and screened, wherein the strain is classified and named Chryseobacterium sp, is preserved in the China General Microbiological Culture Collection Center, and has the preservation number of CGMCC NO.10699; and the Chryseobacterium sp can effectively degrade picolinic acid in a wide pH value range or wide temperature range or in a wide pH value range and wide temperature range, can be adopted as a biological strengthening preparation so as to be used in aerobic biological treatment of picolinic acid wastewater within a weak alkali to weak acidity range and a wide temperature range, and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a strain of Chryseobacterium efficiently degrading picolinic acid and its application. Background technique [0002] Picolinic acid and its derivatives are widely used in the production of medicines, pesticides and daily chemicals, and the widespread use of picolinic acid leads to environmental pollution. Picolinic acid wastewater can be treated by aerobic biological method. At present, the microbial resources for aerobic degradation of picolinic acid mainly include Arthrobacter, Mycobacterium and Streptomyces. There are few reports on strains of other genera. [0003] The most important use of picolinic acid in industry is the production of medicines and pesticides. The resulting wastewater has a high acidity and a pH value between 4 and 6. Under strong acidic conditions, the growth of picolinic acid-degrading bacteria reported in the literature is signific...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC02F3/34C02F2101/38C12R2001/01C12N1/205
Inventor 郑春莉沈振兴何炽刘萍萍刘红霞冯珊珊王巧蕊
Owner XI AN JIAOTONG UNIV
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