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Algicidal Chryseobacterium sp. and its application in cyanobacterial bloom control

A technology of Chryseobacterium aureus and cyanobacteria bloom, applied in the field of environmental microorganisms, can solve problems such as limited processing capacity and secondary pollution

Inactive Publication Date: 2014-09-17
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the limited processing capacity of physical methods and chemical methods in the prior art, and the defects of easily causing secondary pollution, the technical problem to be solved by the present invention is to find efficient algae-dissolving bacteria and to isolate and enrich the highly efficient algae-dissolving active substances produced by the metabolism of algae-dissolving bacteria , for safe and efficient control of cyanobacterial blooms

Method used

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  • Algicidal Chryseobacterium sp. and its application in cyanobacterial bloom control
  • Algicidal Chryseobacterium sp. and its application in cyanobacterial bloom control
  • Algicidal Chryseobacterium sp. and its application in cyanobacterial bloom control

Examples

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Embodiment 1

[0043] Example 1 Screening of algae-lytic bacteria

[0044] Add 2mL of natural water samples collected from the Meiliangwan waters of Taihu Lake to 98mL of algae liquid of Microcystis aeruginosa 9110 in the logarithmic phase. Two days later, take the yellow algae liquid and use the gradient dilution method to coat the beef extract peptone medium agar plate and culture at 28°C. After 24 hours, take a plate with a moderate colony density, and select different strains according to the different colony shapes.

[0045] The screened strains were inoculated into 10 mL of beef extract peptone medium, cultured at 28°C and 220 rpm for 24 hours, and 2 mL of cultured bacterial liquid was added to 98 mL of algae liquid of Microcystis aeruginosa in logarithmic phase. In addition, 2 mL of sterilized beef extract peptone medium was also added to 98 mL of algae liquid as a control. All the algae fluids of the experimental group and the control group were cultured in the light incubator for 4...

Embodiment 2

[0050] Identification of embodiment 2 Chryseobacterium aureus GLY-4205 bacterial strains

[0051] GLY-4205, which has the strongest algicidal effect, was identified by morphological observation, staining, physiological and biochemical reactions, and 16srRNA gene sequence analysis. ~4.0 μm, after 24 hours of shaker culture in liquid medium, individual cells can reach 8 μm. After being cultured on the nutrient agar solid medium plate for 24 hours, the colony shape is round, the surface is smooth and flat, the edges are neat, yellow, and the colony size is 2mm to 3mm. After 16srRNA gene sequence analysis and homology comparison, it was known that it had 99% homology with a certain strain of Chryseobacterium in GenBank, so it was identified as a bacterium of the genus Chryseobacterium and named Chryseobacterium GLY-4205.

Embodiment 3

[0052] Embodiment 3 The preparation method of Chryseobacterium aureus GLY-4205 fermented liquid and ethyl acetate extract thereof

[0053] Inoculate Chryseobacterium GLY-4205 in the sterilized beef extract peptone medium with pH 7.0 according to 1% inoculum amount, and culture it on a shaker at 220 rpm at 28°C for 48 hours to obtain the bacterium-containing fermentation liquid of Chryseobacterium GLY-4205, and the above fermentation liquid Centrifuge at 8,000×g for 30 minutes to collect the supernatant. Add ethyl acetate to the supernatant of the fermentation broth at a ratio of 1:1, put it into a shaker and vibrate for 24 hours, and separate the upper layer solution, that is, the ethyl acetate extract. The ethyl acetate was evaporated to dryness, dissolved in water, and filtered through a microporous membrane with a pore size of 0.22 μm for further purification of metabolites.

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Abstract

The invention discloses an algicidal Chryseobacterium sp. and its application in cyanobacterial bloom control. Highly algicidal Chryseobacterium sp. GLY-4205 is separated from water in Taihu Lake, and has a preservation number of CGMCC No.8283, and an identification result of effective algicidal components separated and purified from a fermentation product of the highly algicidal Chryseobacterium sp. GLY-4205 show that the effective algicidal components comprise 3-isopropyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione, 7-hydroxy-3-isobutyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)-pyrrolo[1,2-a]pyrazine-1,4-dione and hexahydropyrrolo[1,2-a]pyrazine-1,4-dione. The half lethal doses LD50 of the above four algicidal substances on Microcystis aeruginosa 9110 are 3.9[mu]g / mL, 0.95[mu]g / mL, 2.82[mu]g / mL, and 5.7[mu]g / mL respectively. Hexahydropyrrolo[1,2-a]pyrazine-1,4-dione has no lethal effect on synechococcus BN60, and the half lethal doses LD50 of the residual three algicidal substances on the half lethal doses are 17.19[mu]g / mL, 1.31[mu]g / mL and 3.84[mu]g / mL respectively. The algicidal Chryseobacterium sp. can be used for developing and producing novel biological algicides in order to be finally applied in the cyanobacterial bloom control of lakes.

Description

technical field [0001] The invention relates to the field of environmental microorganisms, in particular to a strain of Chryseobacterium sp. GLY-4205 with algae-dissolving activity and its application in the control of cyanobacteria blooms. Background technique [0002] In recent years, cyanobacteria blooms caused by lake eutrophication have polluted lake water systems more and more seriously, and freshwater areas such as Taihu Lake, Chaohu Lake, and Dianchi Lake have been seriously affected. Therefore, it is very necessary to explore effective ways to control the cyanobacterial biomass and inhibit the occurrence of cyanobacterial blooms. [0003] The control technology of cyanobacteria bloom can be summarized as physical method, chemical method and biological method. Physical methods such as mechanical algae removal, electromagnetic field algae removal, etc. can be used as auxiliary control measures for algal blooms, but the disadvantages are that they treat the symptoms b...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34A01N63/00A01N43/90A01P13/00C12R1/01
Inventor 杨虹郭星亮
Owner SHANGHAI JIAO TONG UNIV
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