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Microorganism for promoting black nightshade to remove trace cadmium pollution in water and method for removing cadmium pollution

A technology for microorganisms and water removal, applied in the field of microorganisms, can solve the problems of poor reusability, complex process, secondary pollution, etc., and achieve the effects of large application potential, good removal effect, and easy operation.

Inactive Publication Date: 2012-07-18
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Commonly used methods to remove cadmium pollution in drinking water mainly include: flocculant filtration method, lime precipitation method, ion exchange method, electrolysis method, etc., but these methods have secondary pollution, high cost, and repeated use during the treatment process. Weakness, complex process and other defects

Method used

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  • Microorganism for promoting black nightshade to remove trace cadmium pollution in water and method for removing cadmium pollution
  • Microorganism for promoting black nightshade to remove trace cadmium pollution in water and method for removing cadmium pollution
  • Microorganism for promoting black nightshade to remove trace cadmium pollution in water and method for removing cadmium pollution

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Germination of nightshade seeds, inoculation with Chryseobacterium sp.LKS03 and hydroponic culture of plants:

[0028] 1. Solanum solanum seeds are soaked in 75% alcohol solution for 15 seconds to sterilize the surface, rinsed with water for 3 times, dried and used for Chryseobacterium sp.LKS03 inoculation.

[0029] 2. Soak the sterilized Solanum nigrum seeds in Chryseobacterium sp.LKS03 inoculum solution for 2 hours, take them out and use them for sowing.

[0030] 3. Spread the vermiculite in a tray with a thickness of about 5 cm, pour it thoroughly with 1 / 4 concentration of modified Hoagland's solution, and sow nightshade seeds in it. It will germinate in about 2-3 days, and the black nightshade can grow to a height of 10 cm in about 2 weeks and be transferred to hydroponics.

[0031] The improved Hoagland solution with a concentration of 4.1 / 4 is also used as a hydroponic nutrient solution, and the nutrient solution is changed every 5 days and replenished from time ...

Embodiment 2

[0033] Chryseobacterium sp.LKS03 has a high ability to produce ACC (1-aminocyclopropane-1-carboxylic acid) deaminase and IAA (indole acetic acid), which can promote the growth of plant roots. On the 45th day and the 80th day of the growth cycle, inoculated Chryseobacterium sp.LKS03 plants and plants without inoculation collected 5 samples respectively, and measured root length and root weight. The results are shown in Table 2:

[0034] Table 2

[0035]

[0036] On the 45th day of the growth cycle, the root length and root weight of Solanum nigrum plants inoculated with Chryseobacterium sp.LKS03 increased by 21.8% and 25.8%, respectively; The length and root weight increased by 25.7% and 38.8%, respectively. The experimental results showed that the inoculation of Chryseobacteriumsp.LKS03 had a significant effect on the root growth of Solanum nigrum.

Embodiment 3

[0038] Tap water was added with heavy metal ions to simulate polluted drinking water. The concentration of cadmium in the simulated polluted drinking water was 50 μg / L. The ability of inoculated nightshade and non-inoculated nightshade to purify the simulated contaminated drinking water was compared. 1 liter of simulated polluted drinking water was poured into the water tank. Solanum nigrum was fixed above the water tank, and the roots were in contact with the water body. The change of cadmium concentration in the water body was tracked and detected within 16 hours. The cadmium concentration was measured by an atomic spectrophotometer. The water purification effect is as follows: image 3 Shown: From the experimental results, it can be seen that Solanum nigrum inoculated with Chryseobacteriumsp.LKS03 will reduce the cadmium content in drinking water to below the national standard (<10μg / L) within 3 hours, and Solanum nigrum not inoculated with endophytic bacteria will take 4.5 h...

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Abstract

The invention provides a microorganism for promoting black nightshade to remove trace cadmium pollution in water and a method for removing cadmium pollution, aiming to purify drinking water subjected to cadmium pollution by virtue of the high-efficiency absorption characteristic of super-accumulated plants, namely black nightshade, to cadmium. In the method, the black nightshade is cultured in anaquaculture manner, and Chryseobacterium sp. LKS03 is inoculated to the black nightshade to promote the roots of the black nightshade to grow; and during purification of drinking water, the roots of black nightshade plants are immersed into the drinking water subjected to cadmium pollution, and each black nightshade plant correspondingly purifies about 1-1.2L of the drinking water in a purification cycle, wherein the purification time is for 2-12h according to the cadmium ion concentration (not higher than 200mug / L) in a water sample, and the capability of the black nightshade plants for purifying the water sample is optimum in the 45th-80th day in the culture cycle of the black nightshade. The method is specific to the cadmium pollution in the drinking water and has the advantages of good removal effect, simplicity and convenience for operation, wide applicable range, and great application potential in the aspect of drinking water purification.

Description

technical field [0001] The invention relates to a biological purification method for drinking water polluted by trace amounts of cadmium, in particular to a method for removing trace amounts of cadmium pollution in water by using black nightshade and the microorganisms used. Background technique [0002] Industrial development is often accompanied by environmental pollution. The problem of heavy metal pollution in water caused by the discharge of waste water and residue in the "three industrial wastes" is becoming more and more serious, which not only destroys the ecological environment, but also directly or indirectly endangers people's health. Heavy metal cadmium pollution mainly comes from printing and dyeing, pesticides, ore mining, smelting and other industries. Long-term intake of trace amounts of cadmium will seriously damage the liver, kidney and other organs of the human body, and cause various diseases. Osteopathy caused by cadmium pollution in Europe and Japan is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02C02F3/32
Inventor 罗胜联陈觉梁陈亮饶婵李波刘承斌
Owner HUNAN UNIV
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