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Isolated soybean protein chitosan nano gel and preparation method and application thereof

A nanotechnology of soybean protein isolate and chitosan, which is applied in the preparation method, application, and processing of plant protein, etc., can solve the problems of low solubility, limited stability and bioavailability, and achieve simple operation and easy use volume and cost reduction

Active Publication Date: 2019-11-12
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above problems, the present invention utilizes chitosan (CS) and soybean protein isolate (SPI) to have opposite charges, so that electrostatic interaction can occur, and the characteristics of forming nanocomposites through ion cross-linking have synthesized a soybean protein isolate Chitosan nanogel, good quality, safe and non-toxic, can effectively solve the problems of low solubility, stability and limited bioavailability of plant essential oils and DHA / EPA in water, and can be widely used in functional food and other fields

Method used

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  • Isolated soybean protein chitosan nano gel and preparation method and application thereof
  • Isolated soybean protein chitosan nano gel and preparation method and application thereof
  • Isolated soybean protein chitosan nano gel and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0037] A method for preparing soybean protein isolate chitosan nanogel and then preparing Pickering emulsion:

[0038] (1) Preparation of CS and SPI solutions:

[0039] Dissolve chitosan in 0.1% acetic acid solution and stir for 10 hours to make a chitosan solution with a concentration of 0.6 mg / ml, then adjust the pH of the chitosan solution to 5.1; dissolve soybean protein isolate in ultrapure water Prepare a 1.6% concentration of SPI solution, add 0.02% sodium azide, stir for 2 hours, put it in a refrigerator at 4°C for overnight hydration, and then centrifuge the soybean protein isolate solution at 8000r for 25 minutes, adjust its pH to 6.6, and further process membrane to remove insoluble material.

[0040] (2) Preparation of SPI / CS nanogel:

[0041] Under 700r / min magnetic stirring, the chitosan solution was continuously added dropwise to the soybean protein isolate solution at a speed of 1ml / min according to SPI:CS(v / v)=2:1, and stirred for 1h.

[0042] (3) Preparati...

Embodiment 2

[0045] A method for preparing soybean protein isolate chitosan nanogel and then preparing Pickering emulsion:

[0046] (1) Preparation of CS and SPI solutions:

[0047] Chitosan was dissolved in 0.1% acetic acid solution and stirred for 10h to make a chitosan solution with a concentration of 0.7mg / ml, and then the pH of the chitosan solution was adjusted to be 5.2; the soybean protein isolate was dissolved in ultrapure water 1.8% concentration of SPI solution was prepared in the mixture, 0.02% sodium azide was added, stirred for 2 hours, placed in a 4°C refrigerator for overnight hydration, and then the soy protein isolate solution was centrifuged at 8000r for 25 minutes to adjust its pH to 6.8, and further membrane to remove insoluble material.

[0048] (2) Preparation of SPI / CS nanogel:

[0049] Under 700r / min magnetic stirring, the chitosan solution was continuously added dropwise to the soybean protein isolate solution at a speed of 1ml / min according to SPI:CS(v / v)=1:1, ...

Embodiment 3

[0053] A method for preparing soybean protein isolate chitosan nanogel and then preparing Pickering emulsion:

[0054] (1) Preparation of CS and SPI solutions:

[0055] Dissolve chitosan in 0.1% acetic acid solution and stir for 10 hours to make a chitosan solution with a concentration of 0.8 mg / ml, then adjust the pH of the chitosan solution to 5.3; dissolve soybean protein isolate in ultrapure water Make a 2.0% concentration of SPI solution, add 0.02% sodium azide, stir for 2 hours, put it in a refrigerator at 4°C for overnight hydration, and then centrifuge the soybean protein isolate solution at 8000r for 25 minutes, adjust its pH to 7.0, and further process membrane to remove insoluble material.

[0056] (2) Preparation of SPI / CS nanogel:

[0057] Under 700r / min magnetic stirring, the chitosan solution was continuously added dropwise to the soybean protein isolate solution at a speed of 1ml / min according to SPI:CS(v / v)=5:8, and stirred for 1h.

[0058] (3) Preparation ...

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Abstract

The invention relates to the technical field of Pickering emulsions, and particularly relates to an isolated soybean protein chitosan nano gel and a preparation method thereof and an application of the isolated soybean protein chitosan nano gel in preparation of Pickering emulsions. The preparation method comprises the following steps: using isolated soybean protein and chitosan as raw materials,and performing complex coacervation with protein polysaccharide to obtain SPI / CS suspension liquid; lyophilizing the suspension liquid to obtain generated SPI / CS powder; dispersing the SPI / CS powder in ultrapure water to form SPI / CS suspension liquid, afterwards adding corn oil to the above suspension liquid, and conducting high-speed dispersion to obtain the stable Pickering emulsion. The preparation method for Pickering emulsions has characteristics of simple preparation process, high stability of the emulsion, less emulsifier dosage, low irritation and low toxicity, environmental friendliness and insusceptibility to various factors of system environment, and can effectively solve the problems that plant essential oils and DHA / EPA have low solubility in water and limited stability and bioavailability, facilitates to reasonably design a delivery system based on food-grade emulsion and has good application prospects.

Description

technical field [0001] The invention relates to the technical field of Pickering emulsions, in particular to a soybean protein isolate chitosan nanogel, a preparation method thereof and an application in preparing Pickering emulsions. Background technique [0002] Pickering emulsion is a new type of emulsion stabilized by solid particles. The solid particles are tightly adsorbed on the oil-water two-phase interface, forming a wrapping layer to wrap the oil droplets to prevent the polymerization between the oil droplets, thereby preventing the collision between the droplets and Coalesce to stabilize the emulsion. At present, there are a large number of biologically active substances, such as plant essential oils, DHA / EPA, etc., which are easy to oxidize and have limited availability in the human body. The solid interfacial particle layer of Pickering emulsion is expected to become a new type of encapsulation or delivery carrier for active compounds. Storage of oil in emulsio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/16C07K1/107A23L29/30A23L29/00
CPCA23J3/16A23L29/045A23L29/30A23V2002/00C07K1/107A23V2250/5488A23V2250/511A23V2250/18
Inventor 刘炳杰杨寒孟祥红
Owner OCEAN UNIV OF CHINA
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