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Preparation method and application of physiologically active notoginseng polysaccharide

A technology of notoginseng polysaccharide and physiological activity, which is applied in the field of natural medicine preparation, can solve the problems of affecting the quality of end products, lack of purification and refining, and low purity of notoginseng polysaccharide, and achieves significant physiological activity, low cost, and easy The effect of industrial production

Active Publication Date: 2012-07-18
YUXI WINHEY BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The produced Panax notoginseng polysaccharide has low purity, high content of heavy metals (especially arsenic) and ash, which affects the quality of the end product
Chinese patent discloses the extraction and separation method of Panax notoginseng polysaccharide (application number 01108493.6 and CN1329094A), which is the preparation method of Panax notoginseng crude polysaccharide without purification and refinement

Method used

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  • Preparation method and application of physiologically active notoginseng polysaccharide
  • Preparation method and application of physiologically active notoginseng polysaccharide
  • Preparation method and application of physiologically active notoginseng polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Take 1 kg of Panax notoginseng main root, crush it, heat and extract it with 8 kg of water at 80° C., extract three times, combine the extracts, cool, and centrifuge at 9000 rm for 10 minutes. The centrifuged supernatant was purified with pretreated polystyrene-type macroporous adsorption resin OU-291, the column flow rate was 0.3BV / h, washed with 1.0 BV purified water equivalent to the column volume, and the flow rate was 1.5 BV / h. h, combined eluents. The eluate passes through an aromatic polyamide flat ultrafiltration membrane with a molecular weight cut-off of 300 and a relative density of the retentate of 1.02. Add 3 times the amount of ethanol to the retentate, let it stand for 1 hour, precipitate, and centrifuge. The precipitate was dissolved in water until the relative density of the retentate was 1.02, and spray-dried to obtain 45 g of Panax notoginseng polysaccharide. The prepared Panax notoginseng polysaccharide was processed by high-temperature ashing meth...

Embodiment 2

[0074] Take 1 kg of Panax notoginseng, crush it, extract it three times with ethanol with a mass concentration of 70%, heat the residue to 90°C for three times, and extract three times with 6 kg of water each time, combine the extracts, cool, and centrifuge at a speed of 5000rm, centrifuge for 15 minutes, centrifuge The final supernatant was purified with pretreated cation exchange resin, the column flow rate was 1.0 BV / h, washed with 2.0 BV purified water equivalent to the volume of the chromatography column, the flow rate was 0.5 BV / h, combined eluents, and washed with particles 40 g of activated carbon was stirred at 60°C for 30 minutes for decolorization, and the clear liquid was obtained by filtration. The clear liquid passed through a cellulose acetate ultrafiltration membrane with a molecular weight cut-off of 500, and the relative density of the retained liquid was 1.03. Add 5 times the amount of ethanol to the retentate, let it stand for 1 hour, precipitate, and centri...

Embodiment 3

[0076] Take 1kg of Panax notoginseng bars, crush them, heat and extract with 7kg of water at 100°C, extract three times, combine the extracts, cool, and centrifuge at 10000rm for 10 minutes. The supernatant after centrifugation was purified with pretreated polystyrene macroporous adsorption resin LAR-714, the column flow rate was 1.0 BV / h, and the purified water equivalent to the volume of the chromatography column was washed with 2.0 BV at a flow rate of 0.8 BV / h. h, combined eluents. The eluate passes through an aromatic polyamide flat ultrafiltration membrane with a molecular weight cut-off of 400 and a relative density of the retentate of 1.03. Add 3 times the amount of ethanol to the retentate, let it stand for 1 hour, precipitate, and centrifuge. The precipitate was dissolved in water until the relative density of the retentate was 1.02, and spray-dried to obtain 42 g of Panax notoginseng polysaccharide. The prepared Panax notoginseng polysaccharide was processed by hi...

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Abstract

The invention provides a preparation method and application of physiologically active notoginseng polysaccharide. The method comprises the steps of refining and purifying by applying resin column chromatography, activated carbon and membrane separation and purification technology, and the like, so as to obtain the notoginseng polysaccharide. The notoginseng polysaccharide obtained by the preparation method provided by the invention has the characteristics of low protein, heavy metal and ash contents and stable quality, has the physiological activities of immunity regulation, liver injury protection, blood glucose decrease, tumor resisting and the like, and can be used singly or combined as raw materials for natural medicines and health products. The preparation method of the notoginseng polysaccharide, provided by the invention, is simple to operate, applicable for industrial production and low in cost and reaches the purposes of energy saving and consumption reduction and realizing comprehensive development and sustainable utilization of the rare Chinese traditional medicine, namely, notoginseng.

Description

technical field [0001] The invention relates to the technical field of natural medicine preparation, in particular to a preparation method and application of a physiologically active Panax notoginseng polysaccharide. Background technique [0002] Panax notoginseng ( Panax notoginseng (Burk.) F.H.Chen), also known as Tianqi, Jinbuhuan, etc., belongs to the Araliaceae (Araliaceae) genus Panax ( Panax )plant. Panax notoginseng is a famous and precious traditional Chinese medicine with a long history of utilization. The main active ingredient of Panax notoginseng is saponin, which has been industrially produced and a variety of products have been developed. Modern pharmacological studies have shown that in addition to saponins, Panax notoginseng polysaccharides also have significant physiological activities. At present, the extraction of Panax notoginseng polysaccharide mostly adopts simple extraction, alcohol precipitation, concentration, drying and other steps. The produ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P37/02A61P1/16A61P19/08A61P35/00A61P3/10A61P31/12A61L27/20A61L27/54A23L1/09
CPCY02P20/10
Inventor 杨崇仁丁艳芬李江霞王东张颖君
Owner YUXI WINHEY BIO TECH
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