Non-antibiotic sucking pig milk replacer and preparation method

A technology for teaching trough feed and suckling pigs, applied in feed, animal feed, animal feed, etc., can solve the problem of not taking into account the influence of synergy and processing technology functions, tributyrin can not completely replace antibiotics, etc. , to achieve the effect of improving animal production performance, enhancing immune function and anti-disease ability, and improving activity

Active Publication Date: 2014-02-26
哈尔滨大北农牧业科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although the use of tributyrin as a feed additive can regulate the gastrointestinal tract of weaned piglets and improve feed utilization, but relying on tributyrin alone cannot completely replace antibiotics, and at the same time, other factors are not considered. The synergistic effect of raw materials and additives with tributyrin and the effect of processing technology on its function

Method used

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  • Non-antibiotic sucking pig milk replacer and preparation method
  • Non-antibiotic sucking pig milk replacer and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Preparation of Bacillus subtilis powder

[0038] 1. Preparation of Bacillus subtilis CGMCC No.6511 live bacteria preparation

[0039] 1) Preparation of Bacillus subtilis seed solution: Inoculate the preserved strains of the test tube slope into a test tube containing 15 mL of culture medium, and cultivate in a constant temperature shaker at 37°C for 16-20 hours, rotating at 225 rpm, until the number of viable bacteria reaches 10 7 CFU / mL, inoculate 1% into a 250mL Erlenmeyer flask containing 100mL seed culture medium, culture in a constant temperature shaker at 37°C for 16-20h, the shaker speed is 225rpm, until the number of viable bacteria reaches 10 7 CFU / mL, as a seed solution for later use.

[0040] The seed medium is: glucose, 20.0g / L; peptone, 10.0g / L; yeast powder, 4.0g / L; sodium chloride, 5g / L; distilled water to 1000mL, pH 7.0±0.2.

[0041] 2) Liquid submerged fermentation culture

[0042] Use a 5L vertical fermentor for liquid fermentation culture. The inocul...

Embodiment 2

[0048] Example 2 Preparation of Lactobacillus acidophilus powder

[0049] 1) Preparation of Lactobacillus acidophilus CGMCC No.6499 seed solution: The Lactobacillus acidophilus species stored in the glycerol cryopreservation tube is streaked on the MRS agar medium plate with an inoculating needle, and incubated at 37°C for 20 hours. Then, the single colony grown on the plate was inoculated into a 250 mL Erlenmeyer flask containing 100mLMRS liquid medium, and incubated at 37°C for 20h. Streak the cultured bacteria liquid on the MRS medium plate again. After incubating at 37°C for 20 hours, inoculate a single colony into a 250mL conical flask containing 100mLMRS liquid medium, and culture at 37°C for 20 hours. Repeat the above operation 3 times to complete the activation of Lactobacillus acidophilus.

[0050] Preparation of Lactobacillus acidophilus seed liquid: Add the activated Lactobacillus acidophilus bacterial liquid to a 5L fermenter with 5% inoculum. The culture parameters a...

Embodiment 3

[0059] Example 3 Preparation of Pediococcus lactis powder

[0060] 1) Preparation of Pediococcus lactis CGMCC No.6500 seed solution

[0061] Inoculate the preserved strains on the inclined surface of the test tube into an anaerobic tube containing 20 mL of MRS liquid medium, and cultivate at a constant temperature of 37°C for 20 hours until the number of viable bacteria reaches 10 7 CFU / mL, inoculate 1% in a 250mL Erlenmeyer flask containing 100mL MRS liquid medium, and anaerobic culture at 37°C for 20h, until the number of viable bacteria reaches 10 7 CFU / mL (count on MRS agar medium plate) and above, as seed solution for later use.

[0062] 2) Liquid submerged fermentation culture

[0063] The liquid seed liquid of Pediococcus lactis was added to a 50L fermentor at an inoculum of 1%, and the culture parameters were: temperature, 37°C; stirring speed, 50rpm; pH value, 6.0±0.5; culture time, 20h. Add 0.5% of the total fermentation volume of CaCO during the fermentation process 3 , Adj...

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Abstract

The invention discloses a non-antibiotic sucking pig milk replacer, which comprises various raw materials as follows: puffed corn, puffed soybean, plasma protein powder, steamed fish meal, soybean protein concentrate, low protein whey powder, lactose, fermented soybean meal, glucose, cane sugar, salt, anti-oxidant, lysine, threonine, methionine, tryptophan, zinc oxide, butyrin, microecology preparation, compound acidifying agent, compound enzyme preparation, compound vitamin premix and compound mineral matter premix. The invention employs synergism between butyrin, zinc oxide, acidifying agent and microecology preparation, and in the prerequisite that the antibiotics are not used, the product can help pigling to realize early stage weaning and get rid of weaning stress, and adjust sucking pig gastrointestinal tract health, control diarrhea, increase survival rate, and comprehensively improve immunity and disease resistance of sucking pig.

Description

Technical field [0001] The invention belongs to the field of feed and relates to a suckling pig feed, in particular to an antibiotic-free suckling pig teaching trough feed and a preparation method thereof. Background technique [0002] Animals have used antibiotics for six to seventy years. As drugs for treatment, disease prevention, health care and growth promotion, they have played a huge role in promoting the development of animal husbandry, and they are still widely used in animal husbandry. However, with the advancement of science and the continuous improvement of people's understanding of biological evolution, the harm and potential harm of antibiotics to humans has become increasingly clear. [0003] Abuse of antibiotics can disrupt the balance of the animal’s intestinal flora; it can increase bacterial resistance and drug-resistant strains; when antibiotics are not fed, the animal’s resistance is poor, and the normal development of the body is affected; it can cause drug re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/16A23K1/00A23K1/165A23K1/175A23N17/00
Inventor 张静刘明忠沈美英洪云张葳
Owner 哈尔滨大北农牧业科技有限公司
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