Method for promoting growth of atractylodes lancea plant by inoculating arbuscular mycorrhizal fungi

Abstract

The invention belongs to the technical field of planting, and discloses a method for promoting the growth of an atractylodes lancea plant by inoculating arbuscular mycorrhizal fungi, and the inoculating method comprises the following steps: step 1, obtaining the arbuscular mycorrhizal fungi, step 2, preparing a sterilization matrix, step 3, carrying out mixed planting, step 4, preparing a microbial inoculum, and step 5, inoculating the microbial inoculum. According to the method provided by the invention, the mycorrhiza infection rate of the rhizoma atractylodis chinensis root system can reach 100%, and the biomass of the rhizoma atractylodis chinensis is remarkably improved; the dependence of the current year and annual atractylodes lancea on mycorrhiza reaches 36% and 52% respectively, and the dry weight of the whole plant is increased by 56.2% and 108.4% respectively; meanwhile, the root length and the root surface area of the next-year atractylodes lancea inoculated with the inoculant are obviously higher than those of the non-inoculated atractylodes lancea; mycorrhizal seedlings formed by the method have good growth vigor and well-developed root systems, and the adaptability and the survival rate of rhizoma atractylodis chinensis are improved.

Description

Technical Field

[0001] The invention belongs to the technical field of planting, and specifically relates to a method for promoting the growth of atractylodes lancea plants by inoculating arbuscular mycorrhizal fungi. Background Art

[0002] Atractylodes chinensis (DC.) Koidz. is a herb with high medicinal value. It prefers to grow in sunny environments, especially on the sunny side of mountainous and hilly areas. However, the natural growth of Atractylodes chinensis is affected by many factors, including soil quality, moisture, temperature, etc.

[0003] When planting Atractylodes lancea, due to its own limitations, Atractylodes lancea prefers sandy soil, is drought-tolerant but afraid of waterlogging, and avoids continuous cropping. If planted in fields with too sticky soil and poor drainage, or if continuous planting leads to deterioration of the soil microenvironment, it will affect the root growth and nutrient absorption of Atractylodes lancea; the organic matter and mineral elements in the soil are important sources of plant growth. If the soil is poor, the organic matter and nitrogen content are low, and the key mineral elements such as phosphorus are insufficient, the growth of Atractylodes lancea will be restricted and the growth rate will be slow; the roots of Atractylodes lancea are its main organs for absorbing water and minerals. If there are defects in the root structure, such as underdeveloped roots, a small number of root hairs or impaired functions, it will directly affect its ability to absorb soil nutrients. These defects may be caused by genetic factors, poor soil conditions, or pests and diseases;

[0004] As the growth years of Atractylodes lancea increase, the physiological functions of its roots may gradually decline, such as weakened absorption capacity and lower metabolic rate. These changes will lead to a decrease in the efficiency of Atractylodes lancea's absorption and utilization of soil nutrients, resulting in malnutrition; there is a close interaction between the roots of Atractylodes lancea and the soil environment. If the soil conditions are not good, such as unsuitable pH value, low organic matter content, and lack of mineral elements, the root growth and nutrient absorption of Atractylodes lancea will be affected. At the same time, the microbial community in the soil will also affect the root health of Atractylodes lancea, and then affect its nutrient absorption capacity; and relying solely on the root regulation of Atractylodes lancea itself, its effect and efficiency are not great, so this study used inoculation of arbuscular mycorrhizal fungi to transform the planting operations of Atractylodes lancea. Summary of the invention

[0005] The object of the present invention is to provide a method for promoting the growth of Atractylodes lancea plants by inoculating arbuscular mycorrhizal fungi, so as to solve the problems raised in the above background technology.

[0006] In order to achieve the above object, the present invention provides the following technical solution: a method for promoting the growth of Atractylodes macrocephala plants by inoculating arbuscular mycorrhizal fungi, the inoculation method comprising the following steps:

[0007] Step 1, obtaining arbuscular mycorrhizal fungi: from the root zone soil with fine roots around the root system of wild Atractylodes lancea containing arbuscular mycorrhizal fungi, the fine roots are cut into small pieces and mixed with the soil to obtain a root-soil mixture;

[0008] Step 2, preparing a sterilized matrix: mixing zeolite, river sand, and quartz sand in a volume ratio of 1:1:2 and sterilizing to obtain a sterilized matrix;

[0009] Step 3, mixed planting: after mixing the root-soil mixture with the sterilized substrate in a volume ratio of 1:1, the mixture is placed in a culture container, and the host plant is sown; the culture container is placed in a solar greenhouse for cultivation, the greenhouse temperature is controlled at 15-30° C., and natural light is provided, and the host plant is cultivated until the infection rate of the arbuscular mycorrhizal fungi of the host plant reaches more than 75% and the arbuscular mycorrhizal fungi spores are mature;

[0010] Step 4, preparing a microbial agent: crushing the root system of the host plant as a component of the microbial agent to obtain a microbial agent for cultivating Atractylodes lancea; the microbial agent may also include a culture matrix: mineral materials and plant materials to provide nutrients and microenvironment required for the growth of mycorrhizal fungi;

[0011] Step five, inoculating the bacterial agent: inoculating the bacterial agent into the matrix to obtain a seedling matrix, and growing Atractylodes lancea seedlings in the seedling matrix to obtain mycorrhized Atractylodes lancea seedlings.

[0012] Preferably, the quartz sand is uniformly mixed in a mass ratio of coarse sand, medium sand and fine sand of 1:2:1; the sterilization method is: using high-pressure steam sterilization, sterilizing twice at 121°C for 40 minutes, with an interval of 24 hours each time, to ensure the sterility of the matrix.

[0013] Preferably, the seedling culture medium is preferably a mixed medium with a volume ratio of peat: vermiculite:perlite of 6:3:1;

[0014] Preferably, the collection site for obtaining arbuscular mycorrhizal fungi is selected from the root system of wild Atractylodes lancea located in Zhongguan Town, Longhua County, Chengde City, Hebei Province, preferably around wild Atractylodes lancea plants of three years old or above, and the root zone soil with fine roots is dug.

[0015] Preferably, in the step 1, the fine roots of the arbuscular mycorrhizal fungi are cut into small segments, usually with a size of 1-2 cm.

[0016] Preferably, in the mixed planting in step 3, the culture container is usually a 1-liter culture plastic pot, and 15 clover and 10 green onions are sown in each pot to ensure that the sowing is even;

[0017] The culture plastic pots are placed in a solar greenhouse with the temperature controlled at 20-25℃ during the day and 15-20℃ at night to simulate the optimal growth environment; natural light is used to ensure at least 8 hours of light per day.

[0018] Preferably, the step 4 of preparing the microbial agent further comprises: when the infection rate of the arbuscular mycorrhizal fungi of the host plant reaches the requirement, carefully taking out the root system of the host plant from the culture matrix, cutting it into small pieces, and using them as components of the microbial agent;

[0019] Dry the prepared bacterial agent at room temperature 20-25℃ until there is no free moisture, and then store it in a cool and dry place for later use.

[0020] Preferably, the step 5 of inoculating the bacterial agent also includes: obtaining a seedling culture medium and mixing it evenly, and adjusting the humidity of the medium to a water holding capacity of 60%-70%.

[0021] Preferably, the fifth step of inoculating the microbial agent also includes the step of planting the microbial agent:

[0022] S1, evenly spread the prepared bacterial agent on the surface of the seedling substrate, and then gently turn the substrate to fully mix the bacterial agent with the substrate;

[0023] S2, sow the seeds of Atractylodes lancea in the seedling medium and cover with a thin layer of soil; keep the medium moist, control the temperature at 20-25℃, and the light time is not less than 8 hours a day; regularly observe and record the growth of Atractylodes lancea seedlings.

[0024] The beneficial effects of the present invention are as follows:

[0025] 1. The method proposed in the present invention can make the mycorrhizal infection rate of the root system of Atractylodes lancea reach 100%, and significantly increase the biomass of Atractylodes lancea. The dependence of the current year and one-year-old Atractylodes lancea on mycorrhizae reaches 36% and 52% respectively, and the dry weight of the whole plant increases by 56.2% and 108.4% respectively. At the same time, the root length and root surface area of ​​the second-year Atractylodes lancea inoculated with the microbial agent are significantly higher than those without inoculation. The mycorrhizal seedlings formed by this method have good growth potential and well-developed root system, which improves the adaptability and survival rate of Atractylodes lancea.

[0026] 2. The present invention achieves natural and eco-friendly promotion of Atractylodes macrocephala plants by obtaining and cultivating arbuscular mycorrhizal fungi from the natural environment, avoiding excessive use of chemical fertilizers and pesticides, and is beneficial to environmental protection and sustainable development. Secondly, arbuscular mycorrhizal fungi can form a symbiotic relationship with Atractylodes macrocephala plants, significantly improving the plant's absorption and utilization efficiency of soil nutrients, including key nutrients such as water, nitrogen, and phosphorus, thereby promoting the healthy growth and development of the plant and improving yield and quality.

[0027] 3. This method optimizes the interaction between arbuscular mycorrhizal fungi and host plants by precisely controlling environmental conditions during the inoculation process, such as temperature and light, ensuring a high infection rate and spore maturity, and further enhancing the inoculation effect. Finally, the prepared inoculant not only contains active arbuscular mycorrhizal fungi, but also adds culture matrix such as mineral materials and plant materials, which provides rich nutrition and a suitable microenvironment for mycorrhizal fungi, helps maintain its activity and stability, and thus achieves a continuous and stable promotion effect on Atractylodes macrocephala plants.

[0028] In summary, the method of promoting the growth of Atractylodes lancea plants by inoculating arbuscular mycorrhizal fungi has multiple advantages such as eco-friendliness, efficient nutrient utilization, optimized interaction environment and sustained promotion effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 The present invention is a flow chart of the method for inoculating arbuscular mycorrhizal fungi. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] like Figure 1 As shown, the embodiment of the present invention provides a method for inoculating arbuscular mycorrhizal fungi to promote the growth of Atractylodes macrocephala plants, and the inoculation method comprises the following steps:

[0032] Step 1, obtaining arbuscular mycorrhizal fungi: from the root zone soil with fine roots around the root system of wild Atractylodes lancea containing arbuscular mycorrhizal fungi, the fine roots are cut into small pieces and mixed with the soil to obtain a root-soil mixture;

[0033] Step 2, preparing a sterilized matrix: mixing zeolite, river sand, and quartz sand in a volume ratio of 1:1:2 and sterilizing to obtain a sterilized matrix;

[0034] Step 3, mixed planting: after mixing the root soil mixture with the sterilized substrate in a volume ratio of 1:1, the mixture is placed in a culture container and the host plant is sown; the culture container is placed in a solar greenhouse for cultivation, the greenhouse temperature is controlled at 15-30° C., and the host plant is exposed to natural light, and the infection rate of the arbuscular mycorrhizal fungi of the host plant reaches more than 75% and the arbuscular mycorrhizal fungi spores are mature (usually 6-8 months);

[0035] Step 4, preparing a microbial agent: crushing the root system of the host plant as a component of the microbial agent to obtain a microbial agent for cultivating Atractylodes lancea; the microbial agent may also include a culture matrix: mineral materials (zeolite, river sand) and plant materials (plant roots) to provide nutrients and microenvironment required for the growth of mycorrhizal fungi;

[0036] Step five, inoculating the bacterial agent: inoculating the bacterial agent into the matrix to obtain a seedling matrix, and growing Atractylodes lancea seedlings in the seedling matrix to obtain mycorrhized Atractylodes lancea seedlings.

[0037] The core of this method is to effectively inoculate arbuscular mycorrhizal fungi through scientific steps to significantly improve the growth performance and yield of Atractylodes lancea. First, wild Atractylodes lancea root soil rich in arbuscular mycorrhizal fungi was selected from the natural environment, and the fungal activity was ensured through meticulous treatment. Subsequently, a mixture of zeolite, river sand and quartz sand was used as a sterilization matrix, which not only provided good air permeability and drainage, but also avoided interference from other microorganisms through a strict sterilization process. During the mixed planting stage, environmental conditions were precisely controlled to ensure full interaction between the host plant and arbuscular mycorrhizal fungi until the ideal infection rate and spore maturity were achieved. Finally, by preparing and applying a microbial agent rich in active fungi, efficient inoculation of Atractylodes lancea seedlings was achieved, laying a solid foundation for later growth.

[0038] The quartz sand is uniformly mixed in a mass ratio of 1:2:1 among coarse sand, medium sand and fine sand; the sterilization method is: high pressure steam sterilization, sterilized twice at 121°C for 40 minutes, with an interval of 24 hours each time, to ensure the sterility of the matrix.

[0039] As an important component of the sterilization matrix, the particle size distribution of quartz sand directly affects the physical properties of the matrix and the microbial growth environment. By accurately mixing coarse sand, medium sand and fine sand, the matrix structure is optimized, which is conducive to the circulation of water and air. The high-pressure steam sterilization method and two consecutive sterilization treatments ensure the sterility of the matrix and effectively prevent contamination during the inoculation process.

[0040] Wherein, the seedling medium is preferably a mixed medium with a volume ratio of peat: vermiculite:perlite of 6:3:1;

[0041] The combination of peat, vermiculite and perlite as a seedling substrate is selected as an ideal choice for Atractylodes macrocephala seedlings due to its good water retention, air permeability and nutrient supply capacity. This substrate ratio helps maintain root health and promotes rapid growth of seedlings.

[0042] Among them, the collection site for obtaining arbuscular mycorrhizal fungi is selected from the root system of wild Atractylodes lancea located in Zhongguan Town, Longhua County, Chengde City, Hebei Province, and preferably around wild Atractylodes lancea plants of three years old or above, and the root zone soil with fine roots is dug.

[0043] The specific area in Zhongguan Town, Longhua County, Chengde City, Hebei Province was selected because of its unique ecological environment and soil conditions, making it an ideal location for obtaining high-quality arbuscular mycorrhizal fungi. In particular, the root soil around wild Atractylodes lancea plants that are three years old or older is rich in mature fungal communities, providing rich resources for inoculation.

[0044] In the step 1, the fine roots of the arbuscular mycorrhizal fungi are cut into small pieces, usually with a length of 1-2 cm.

[0045] Cutting the fine roots into 1-2 cm segments helps increase the contact area between the fungus and the soil and improves infection efficiency. The delicate operation of this step is crucial for the subsequent reproduction and infection of the fungus.

[0046] In the mixed planting of step 3, the culture container is usually a 1-liter culture plastic pot, and 15 clover and 10 green onions are sown in each pot to ensure that the sowing is even;

[0047] One-liter plastic pots were used as containers, and the sowing density was strictly controlled to ensure uniform growth among the host plants. By simulating the optimal growth environment, including temperature, light and other conditions, the best conditions were provided for the interaction between arbuscular mycorrhizal fungi and host plants.

[0048] The culture plastic pots are placed in a solar greenhouse with the temperature controlled at 20-25℃ during the day and 15-20℃ at night to simulate the optimal growth environment; natural light is used to ensure at least 8 hours of light per day.

[0049] Wherein, the step 4 of preparing the microbial agent also includes: when the infection rate of the arbuscular mycorrhizal fungi of the host plant reaches the requirement, carefully taking out the root system of the host plant from the culture matrix, cutting it into small pieces (about 0.5-1 cm), and using it as a component of the microbial agent;

[0050] After the host plant reaches the infection requirement, the root system is carefully processed to prepare the inoculant and its activity is maintained through the drying and storage steps. Each link in this process is crucial and directly affects the quality of the inoculant and the subsequent inoculation effect.

[0051] Dry the prepared bacterial agent at room temperature 20-25℃ until there is no free moisture, and then store it in a cool and dry place for later use.

[0052] Wherein, the step 5 of inoculating the bacterial agent also includes: obtaining a seedling culture matrix and mixing it evenly, and adjusting the humidity of the matrix to a water holding capacity of 60%-70%.

[0053] Adjusting the substrate humidity to 60%-70% of the water holding capacity is the key to ensuring the germination of Atractylodes lancea seeds and the growth of seedlings. A suitable humidity environment is conducive to root development and water absorption, and is an important factor in the success of seedling cultivation.

[0054] Wherein, the fifth step of inoculating the microbial agent also includes the step of planting the microbial agent:

[0055] S1, evenly spread the prepared bacterial agent on the surface of the seedling substrate, and then gently turn the substrate to fully mix the bacterial agent with the substrate;

[0056] S2, sow Atractylodes macrocephala seeds in the seedling medium and cover with a thin layer of soil (about 0.5 cm); keep the medium moist, control the temperature at 20-25°C, and the light time is not less than 8 hours a day; regularly observe and record the growth of Atractylodes macrocephala seedlings.

[0057] The detailed steps of microbial cultivation include uniform sowing, substrate mixing, sowing and covering, etc. Each step is carefully designed to ensure the inoculation effect. At the same time, by regularly observing and recording the growth of seedlings and adjusting management measures in time, a strong guarantee is provided for the healthy growth of Atractylodes lancea.

[0058] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0059] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for promoting the growth of Atractylodes lancea plants by inoculating arbuscular mycorrhizal fungi, characterized in that: The vaccination method includes the following steps: Step 1, obtaining arbuscular mycorrhizal fungi: from the root zone soil with fine roots around the root system of wild Atractylodes lancea containing arbuscular mycorrhizal fungi, the fine roots are cut into small pieces and mixed with the soil to obtain a root-soil mixture; Step 2, preparing a sterilized matrix: mixing zeolite, river sand, and quartz sand in a volume ratio of 1:1:2 and sterilizing to obtain a sterilized matrix; Step 3, mixed planting: after mixing the root-soil mixture with the sterilized substrate in a volume ratio of 1:1, the mixture is placed in a culture container, and the host plant is sown; the culture container is placed in a solar greenhouse for cultivation, the greenhouse temperature is controlled at 15-30° C., and natural light is provided, and the host plant is cultivated until the infection rate of the arbuscular mycorrhizal fungi of the host plant reaches more than 75% and the arbuscular mycorrhizal fungi spores are mature; Step 4, preparing a microbial agent: crushing the root system of the host plant as a component of the microbial agent to obtain a microbial agent for cultivating Atractylodes lancea; the microbial agent may also include a culture matrix: mineral materials and plant materials to provide nutrients and microenvironment required for the growth of mycorrhizal fungi; Step five, inoculating the bacterial agent: inoculating the bacterial agent into the matrix to obtain a seedling matrix, and growing Atractylodes lancea seedlings in the seedling matrix to obtain mycorrhized Atractylodes lancea seedlings.

2. The method for promoting the growth of Atractylodes lancea plants by inoculating arbuscular mycorrhizal fungi according to claim 1, characterized in that: The quartz sand is uniformly mixed in a mass ratio of coarse sand, medium sand and fine sand of 1:2:1; the sterilization method is: using high-pressure steam sterilization, sterilizing twice at 121°C for 40 minutes, with an interval of 24 hours each time, to ensure the sterility of the matrix.

3. The method for promoting the growth of Atractylodes lancea plants by inoculating arbuscular mycorrhizal fungi according to claim 1, characterized in that: The seedling raising matrix is ​​preferably a mixed matrix with a volume ratio of peat: vermiculite:perlite of 6:3:

1.

4. The method for promoting the growth of Atractylodes lancea plants by inoculating arbuscular mycorrhizal fungi according to claim 1, characterized in that: The collection site for obtaining arbuscular mycorrhizal fungi is selected from the root system of wild Atractylodes lancea located in Zhongguan Town, Longhua County, Chengde City, Hebei Province, preferably around wild Atractylodes lancea plants of three years old or above, and the root zone soil with fine roots is dug.

5. The method for promoting the growth of Atractylodes lancea plants by inoculating arbuscular mycorrhizal fungi according to claim 1, characterized in that: In the step 1 of obtaining arbuscular mycorrhizal fungi, the fine roots are cut into small segments usually with a length of 1-2 cm.

6. The method for promoting the growth of Atractylodes lancea plants by inoculating arbuscular mycorrhizal fungi according to claim 1, characterized in that: In the mixed planting of step 3, the culture container is usually a 1-liter culture plastic pot, and 15 clover and 10 green onions are sown in each pot to ensure that the sowing is even; The culture plastic pots are placed in a solar greenhouse with the temperature controlled at 20-25℃ during the day and 15-20℃ at night to simulate the optimal growth environment; natural light is used to ensure at least 8 hours of light per day.

7. The method for promoting the growth of Atractylodes lancea plants by inoculating arbuscular mycorrhizal fungi according to claim 1, characterized in that: The step 4 of preparing the microbial agent also includes: when the infection rate of the arbuscular mycorrhizal fungi of the host plant reaches the requirement, carefully taking out the root system of the host plant from the culture matrix, cutting it into small pieces, and using them as the components of the microbial agent; Dry the prepared bacterial agent at room temperature 20-25℃ until there is no free moisture, and then store it in a cool and dry place for later use.

8. The method for promoting the growth of Atractylodes lancea plants by inoculating arbuscular mycorrhizal fungi according to claim 1, characterized in that: The step 5 of inoculating the bacterial agent also includes: obtaining a seedling culture matrix and mixing it evenly, and adjusting the humidity of the matrix to 60%-70% of the water holding capacity.

9. The method for promoting the growth of Atractylodes lancea plants by inoculating arbuscular mycorrhizal fungi according to claim 1, characterized in that: The inoculation of the microbial agent in step 5 also includes a method for planting the microbial agent: S1, evenly spread the prepared bacterial agent on the surface of the seedling substrate, and then gently turn the substrate to fully mix the bacterial agent with the substrate; S2, sow the seeds of Atractylodes lancea in the seedling medium and cover with a thin layer of soil; keep the medium moist, control the temperature at 20-25℃, and the light time is not less than 8 hours a day; regularly observe and record the growth of Atractylodes lancea seedlings.

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