A method for improving salt tolerance of ice leaf daylily seedlings

Abstract

The invention belongs to the field of botany, and provides a method for improving the salt tolerance of ice leaf daylily seedlings, determines the optimum NaCl concentration for the growth of ice leaf daylily, and while the appropriate amount of NaCl treatment increases the yield of ice leaf daylily seedlings, it also increases the content of various nutrients (protein, soluble sugar, organic acid, dietary fiber and various amino acids) of the seedlings, and at this time, the betalain content is also the highest. Under 0mM (control NaCl concentration), 100mM (optimal NaCl concentration) and 200mM (stress NaCl concentration) NaCl treatments, the optimum nitrogen (7.5mM), phosphorus (1mM), potassium (4mM) and calcium (5mM) concentrations for the growth of ice leaf daylily seedlings are respectively determined. Under the treatments of 0mM, 100mM and 200mMNaCl, a four-factor three-level orthogonal experiment was used to determine the reasonable fertilization scheme for ice leaf helianthus seedlings under salt treatment conditions, that is, nutrient solutions with nitrogen, phosphorus, potassium and calcium of 7.5mM, 1mM, 8mM, 2mM or 10mM, 0.5mM, 8mM, 5mM respectively can significantly promote the growth of ice leaf helianthus.

Description

Technical Field

[0001] The invention belongs to the field of botany, and particularly relates to a method for improving the salt tolerance of ice leaf helianthus annuus seedlings. Background Art

[0002] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention, and should not necessarily be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art.

[0003] Against the backdrop of a changing climate, soil salinization is one of the most common environmental problems. It seriously affects global food security and has become a global ecological problem.

[0004] The most abundant cation in salinized soil is Na + , followed by Mg 2+ , Ca 2+ , K + ; The most abundant anion is Cl - , followed by HCO3 - 、CO3 2- These salt ions in the soil will cause osmotic stress, ion stress and nutritional imbalance to plants, hindering their normal growth. At the same time, a large amount of Na + , Cl - Ions will enter the plant body in a passive way, not only causing ion toxicity to the plant, but also inhibiting the plant's absorption of other nutrients such as Ca 2+ , K + 、NO3 - PO4 3- The absorption of carbon dioxide affects the metabolism in plants, and eventually causes irreversible damage such as impaired photosynthesis and carbon metabolism.

[0005] Ice leaf daylily belongs to the Aizoaceae family and is a salt-secreting halophyte. Ice leaf daylily is not only stress-resistant, but also a very good health vegetable. It not only has a unique flavor, but also contains a large amount of nutrients. It has the effect of lowering blood sugar and improving liver function, so it is becoming more and more popular.

[0006] The paper "Research Progress on Salt Tolerance Mechanism of Ice Leaf Helianthus Annuus" reviews the salt tolerance mechanism of Ice Leaf Helianthus Annuus from the aspects of osmotic regulation, water channel protein regulation and reactive oxygen scavenging.

[0007] The paper "Effects of calcium on ion content in different organs and root K in Euphorbia glabra under salt stress" + 、Na + The study investigated the role of calcium in promoting the absorption of Na in the roots of Euphorbia pulcherrima after salt stress. + Excretion, inhibition of K+ The outflow process keeps the Na in various organs low. + The content shows that calcium plays an important role in maintaining and regulating ion balance.

[0008] The paper "Physiological Responses of Melia glabra under Treatment with Sodium Chloride, Sodium Salt and Chloride Salt" uses Melia glabra as material to study the effects of different concentrations of sodium chloride, sodium salt and chloride salt on its nutritional growth and photosynthetic pathway changes.

[0009] Although the above research has certain reference significance for improving the growth conditions of P. iceleaf under salt stress, since the factors affecting plant growth include water, air, light, soil pH, soil microorganisms and other aspects, it is still necessary to establish a direct correspondence between different factors and the growth indicators of P. iceleaf, so as to better guide the cultivation of P. iceleaf. Summary of the invention

[0010] In order to solve the above problems, the present invention provides a method for improving the salt tolerance of Melia glaucosa seedlings.

[0011] In order to achieve the above object, the present invention adopts the following technical solution:

[0012] The first aspect of the present invention provides NO 3- Application in improving the biomass, chlorophyll, photosynthetic parameters or regulating ion content of ice leaf daylily seedlings under salt stress, wherein the specific conditions of salt stress are: the concentration of NaCl is 50-100mM;

[0013] NO 3- The concentration is 2.5~10mM.

[0014] In a second aspect of the present invention, PO4 3- Application in improving the biomass, chlorophyll, photosynthetic parameters or regulating ion content of ice leaf daylily seedlings under salt stress, wherein the specific conditions of salt stress are: the concentration of NaCl is 50-100mM;

[0015] The PO4 3- The concentration is 0.5~1mM.

[0016] The third aspect of the present invention provides K + Application in improving the biomass, chlorophyll, photosynthetic parameters or regulating ion content of ice leaf daylily seedlings under salt stress, wherein the specific conditions of salt stress are: the concentration of NaCl is 50-100mM;

[0017] The K + The concentration is 4-8mM.

[0018] In a fourth aspect of the present invention, a nutrient solution T5 or T7 is provided for increasing the biomass, chlorophyll, photosynthetic parameters or regulating ion content of Mesona glaucosa seedlings under salt stress, wherein the nutrient solution T5 contains NO 3- PO4 3- , K + , Ca 2+ , where the concentration ratio of N:P:K:Ca is 7.5:1:8:2;

[0019] The nutrient solution T7 contains NO 3- PO4 3- , K + , Ca 2+ , where the concentration ratio of N:P:K:Ca is 10:0.5:8:5.

[0020] A fifth aspect of the present invention provides a method of using PO4 3- , Ca 2+ Application of combined treatment in increasing the content of dibutyl phthalate in Ice-leaved Helianthus annuus.

[0021] Beneficial Effects of the Invention

[0022] (1)With NO 3- PO4 3- , K + , Ca 2+ Compared with single ion treatment, PO4 3- , Ca 2+ The combined treatment can significantly increase the content of dibutyl phthalate in Ice Leaf Daylily, which has a synergistic effect.

[0023] (2) The present invention found that: under the stress of NaCl treatment, NO3 - The treatment significantly promoted the growth of the ice leaf daylily seedlings, as shown by the significant increase in biomass and photosynthesis; the Na + and Cl - The content of K + and NO3 - The content increased significantly, effectively alleviating the damage caused by salt stress to the ice leaf daylily.

[0024] (3) The present invention found that: under NaCl stress, PO4 3- Treatment can increase the biomass, chlorophyll content and photosynthesis of flower seedlings, and the Na + and Cl - The content of K + and PO4 3 Increased content.

[0025] (4) The present invention found that: under NaCl stress, K + Treatment can increase the biomass, chlorophyll content and photosynthesis of flower seedlings, and the Na + and Cl - The content of K + and PO4 3 Increased content.

[0026] (5) The present invention found that under NaCl stress, NO 3- PO4 3- , K + , Ca 2+ The synergistic treatment of the four ions can significantly promote the growth of P. iceleaf and significantly reduce the Na + and Cl - The content of K + 、NO3 - and PO4 3- The content of , improves the ability of seedlings to tolerate salt stress. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The accompanying drawings constituting a part of the present invention are used to provide a further understanding of the present invention, and the exemplary embodiments of the present invention and their description are used to explain the present invention and do not constitute improper limitations on the present invention.

[0028] Figure 1 The different concentrations of NO3 in the present invention - Effects of NaCl treatment on the fresh weight and dry weight of Melia glaucosa seedlings. Note: The data are the mean ± SD of 5 replicates, and different lowercase letters indicate significant differences at the P < 0.05 level using Duncan's multiple test, the same below.

[0029] Figure 2 The different concentrations of NO3 in the present invention - Effect of NaCl treatment on chlorophyll content in ice leaf daylily seedlings.

[0030] Figure 3 The different concentrations of NO3 in the present invention - Effects of NaCl treatment on photosynthetic parameters of Euphorbia obesa seedlings.

[0031] Figure 4 The different concentrations of NO3 in the present invention - NaCl treatment on the Na + , K + impact.

[0032] Figure 5 The different concentrations of NO3 in the present invention -NO3 in the seedlings of Euphorbia pulcherrima under NaCl treatment - , Cl - The influence of content.

[0033] Figure 6 The different concentrations of PO4 3- Effect of NaCl treatment on the fresh weight and dry weight of Melia azedarach seedlings.

[0034] Figure 7 The different concentrations of PO4 in the present invention 3- Effect of NaCl treatment on chlorophyll content in ice leaf daylily seedlings.

[0035] Figure 8 The different concentrations of PO4 in the present invention 3- Effects of NaCl treatment on photosynthetic parameters of Euphorbia obesa seedlings.

[0036] Fig. 9 The different concentrations of PO4 in the present invention 3- NaCl treatment on the Na + , K + impact.

[0037] Fig.10 The different concentrations of PO4 in the present invention 3- PO4 in the seedlings of Euphorbia pulcherrima under NaCl treatment 3- , Cl - The influence of content.

[0038] Fig.11 The different concentrations of Ca 2+ Effect of NaCl treatment on the fresh weight and dry weight of Melia azedarach seedlings.

[0039] Fig.12 The different concentrations of Ca 2+ Effect of NaCl treatment on chlorophyll content in ice leaf daylily seedlings.

[0040] Fig.13 The different concentrations of Ca 2+ Effects of NaCl treatment on photosynthetic parameters of Euphorbia obesa seedlings.

[0041] Fig.14 The different concentrations of Ca 2+ NaCl treatment on the Na + , K + impact.

[0042] Fig.15 The different concentrations of Ca 2+ NO3 in the seedlings of Euphorbia pulcherrima under NaCl treatment- , Cl - The influence of content.

[0043] Fig.16 The different concentrations of K in the present invention + Effect of NaCl treatment on the fresh weight and dry weight of Melia azedarach seedlings.

[0044] Fig.17 The different concentrations of K in the present invention + Effect of NaCl treatment on chlorophyll content in ice leaf daylily seedlings.

[0045] Fig.18 The different concentrations of K in the present invention + Effects of NaCl treatment on photosynthetic parameters of Euphorbia obesa seedlings.

[0046] Fig.19 The different concentrations of K in the present invention + NaCl treatment on the Na + , K + impact.

[0047] Fig. 20 The different concentrations of K in the present invention + NO3 in the seedlings of Euphorbia pulcherrima under NaCl treatment - , Cl - The influence of content.

[0048] Fig.21 The effects of different nitrogen, phosphorus, potassium and calcium ratios on the fresh weight and dry weight of the aboveground part of the ice leaf daylily seedlings under NaCl treatment. Note: The data are the mean ± SD of 5 repeated values, and different lowercase letters indicate significant differences at the P < 0.05 level using Duncan's multiple test, the same below.

[0049] Fig. 22 The present invention discloses the effects of different nitrogen, phosphorus, potassium and calcium ratios on the chlorophyll content in Mesona glaucosa seedlings under NaCl treatment.

[0050] Fig.23 The present invention discloses the effects of different nitrogen, phosphorus, potassium and calcium ratios on photosynthetic parameters of Melia glaucosa seedlings under NaCl treatment.

[0051] Fig.24 The results of the present invention show that different nitrogen, phosphorus, potassium and calcium ratios affect the Na content of the ice leaf daylily seedlings under NaCl treatment. + , K + impact.

[0052] Fig.25 The NO3 of different nitrogen, phosphorus, potassium and calcium ratios of the present invention on the ice leaf daylily seedlings under NaCl treatment - , Cl- PO4 3- impact. DETAILED DESCRIPTION

[0053] It should be noted that the following detailed descriptions are exemplary and are intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used in the present invention have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs.

[0054] The present invention will be further described in detail below in conjunction with specific embodiments. It should be noted that the specific embodiments are intended to explain the present invention rather than to limit it.

[0055] In the following examples, the seeds of P. iceleaf were provided by Professor Qiao Peng of Shandong Sericulture Research Institute and stored in a 4°C refrigerator.

[0056] In the following embodiments, the test methods of each index are as follows:

[0057] Determination of biomass, leaf area and fleshiness

[0058] Determination of fresh weight of aboveground parts: refer to the method (Zhao Chen. Physiological responses of Psoraleaf glaucoma under treatment with sodium chloride, sodium salt and chloride salt [D]. Shandong Normal University, 2021.), and measure its fresh weight (FW).

[0059] Determination of dry weight of aboveground part: Cut the above plant materials into small pieces quickly and mix them well. Weigh 2g of the materials and put them in a clean, dry envelope. Dry them in a 70℃ oven for 2-3 days until the weight is constant. The weight is the dry weight (DW) of the material with a fresh weight of 2g. The total dry weight is calculated based on the water content. Five replicates are measured for each treatment. Water content (%) = (2-DW) / 2×100%

[0060] Total dry weight of above-ground part (g) = FW-(water content × FW)

[0061] Determination of leaf area: After 28 days of NaCl treatment, leaves with consistent growth and position were taken and their leaf areas were measured using a leaf area meter (YMJ-P, Shandong Hengmei Electronic Technology Co., Ltd.), and 5 replicates were measured for each treatment.

[0062] Meat content (g / cm 2)=FW / leafarea, reference method (Rahman MM, Mostofa MG, RahmanM A, Islam MR, Tran L. Acetic acid: a cost-effective agent for mitigation of seawater-induced salt toxicity in mung bean[J]. Scientific Reports, 2019, 15186(9).).

[0063] Determination of ion content

[0064] Accurately weigh 5g of sample, dry it, refer to the method (Wang Guihua, Li Jiawei, Lu Jianhua, Wang Dongying, Guo Jianxiong, Li Haiqiao, Yu Goubin. Monitoring and evaluation of heavy metal elements in soil of Guangdong sugarcane area [J]. Sugarcane Industry, 2014, (01): 54-57.), add 8ml HNO3 and soak overnight; digest on a 200℃ hot plate until 2-3ml of liquid remains. After digestion, dilute to 20ml with 5% HNO3 and set aside. An inductively coupled plasma atomic emission spectrometer (ICP: Agilent ICP-OES710, YS-069) was used to determine the content of different ions at different wavelengths. The wavelength (nm) corresponding to each ion was S / 181.972, B / 249.772, Mn / 257.610, Mo / 202.032, Ni / 231.604, Si / 251.611, Mg / 279.553, Na / 589.592, Cu / 327.395, Ca / 396.847, K / 766.491, Fe / 238.204, and Zn / 213.857. Six replicates were measured for each treatment.

[0065] Determination of nutrient content

[0066] Determination of protein content: Accurately weigh 5g of the thoroughly mixed ice leaf daylily seedling sample into the digestive tube, and refer to the method (Suo Yanyan, Fan Ruizhao, Si Xianzong, Yu Qiong, Sun Yanmin, Mao Jiawei, Li Liang, Yu Hui. Study on the nitrogen, calcium and sulfur fertilization model for high-quality and high-yield peanuts in the sandy black soil area [J]. Journal of Nuclear Agricultural Sciences, 2019, 33(07): 1448-1456), and use a fully automatic nitrogen fixer (automatic liquid addition, distillation, titration and recording of titration data) to determine the protein content. Determination of amino acid content: Use a homogenizer to make the material sample into a homogenate, seal and freeze it for later use; refer to the method (Wang Lili. Amino acid detection method for polypeptide drugs [J]. Science and Technology Innovation and Application, 2014, (16): 272.) to hydrolyze the material and dry it under reduced pressure; after passing through a 0.22μm filter membrane, inject the sample standard solution and the test solution into the amino acid analyzer, and calculate the concentration of each amino acid by the external standard method. Determination of soluble sugar content: Refer to the method (Lu Junwei. Study on the preservation effect of bayberry combined with space electric field and low temperature storage [D]. Zhejiang Ocean University, 2019.), use a pipette to accurately pipette 0, 0.2, 0.4, 0.8, 1 and 1.2mL of glucose standard solution into a 10mL centrifuge tube, add water to 2mL, then add 4mL of 3,5-dinitrosalicylic acid, heat for 5min, and make a standard curve; use a pipette to pipette 5mL of sample solution, add 1mL of 6mol / L hydrochloric acid; add 3 drops of methyl red indicator, neutralize with 6mol / L sodium hydroxide solution to light orange, and determine the absorbance value of the sample according to the above steps.

[0067] Determination of betalain content

[0068] The traditional solvent extraction method, i.e., leaching method, was used to extract betalain from the leaves. According to the reference method (Duan Di. Research on the extraction, separation and purification process of betalain from Suaeda salsa[D]. Shandong Normal University, 2009.), 5 g of fresh leaves were placed in a mortar, liquid nitrogen was added, and the leaves were quickly ground thoroughly. 5 mL of water was added and the leaves were leached at 38°C for 30 min, with a solid-liquid ratio of 1:5 and acidity was ensured; the leaves were centrifuged at 12000 g, the supernatant was retained, and its OD value was measured at a wavelength of 535 nm. BC (mg / L) × total amount of extract (mL) betalain content (mg / g) = sample fresh weight (g) × 1000 BC (mg / L) = (OD × Df × Mw × 1000) / (ε × L), Df is the dilution factor, Mw is the molecular weight of betalain (Mw = 550 g / mol), ε is the molar absorption coefficient of betalain ε = 60000 L / (mol·cm), L is the thickness of the cuvette.

[0069] Determination of chlorophyll content

[0070] Cut off the mature leaves of the same growth and position of the ice leaf sunflower seedlings, immediately wash them with deionized water, wipe them dry, cut them into pieces and mix them evenly, refer to the method (Zhao Chen. Physiological response of ice leaf sunflower under sodium chloride, sodium salt and chloride salt treatment [D]. Shandong Normal University, 2021.) and calculation formula. Measure 5 replicates for each treatment.

[0071] Determination of cation content in plants

[0072] Mature leaves of the same leaf position and growth of Elaeagnus angustifolia seedlings were cut and the Na and N content of the leaves were measured using a FlamePhotometer410 flame spectrophotometer according to the reference method (Lin J, Li JP, Yuan F, Yang Z, Wang BS, Chen M. Transcriptome profiling of genes involved in photosynthesis in Elaeagnus angustifolia L. under salt stress [J]. Photosynthetica, 2018.). + , K + and Ca 2+ Each treatment was repeated 5 times.

[0073] Determination of Anion Content in Plants

[0074] NO3 in plants - Content determination method:

[0075] (1) Prepare 500 mg / L nitrate nitrogen standard solution. Accurately weigh 0.7221 g potassium nitrate, dissolve it in distilled water, and dilute to 200 mL. Take 1, 2, 3, 4, and 5 mL of the standard solution in a 25 mL volumetric flask and dilute to the volume. The concentrations of the standard solutions become 20, 40, 60, 80, and 100 ug / mL, respectively.

[0076] (2) NO3 - For extraction, take 0.3 g of sample material, add 10 ml of deionized water into a test tube, and after boiling in a water bath for 3 hours, dilute the remaining liquid with deionized water and make up to 25 ml.

[0077] (3) Take 0.1 ml of the standard solution or extract, add 0.4 ml of 5% salicylic acid-sulfuric acid solution and 9.5 ml of 8% sodium hydroxide, shake well, and place at room temperature for 20 minutes. The blank control is 0.1 ml of distilled water, and other conditions are the same as the experimental group.

[0078] (4) Reference method (Fu Xinxing. Effect of grafting on nitrate absorption and utilization of watermelon seedlings [D]. Zhejiang University, 2014.), using an ultraviolet spectrophotometer to measure the absorbance value, and making a calibration curve to obtain the regression equation;

[0079] Finally, the NO3 content of each sample was calculated based on the absorbance value and regression equation. - content.

[0080] Cl in plants - Content determination method:

[0081] (1) Preparation of Cl - Standard solution: accurately weigh 0.8243 g NaCl and dilute to 500 mL with DDH2O to obtain a standard Cl solution with a mass concentration of 1 mg / mL. - Solution.

[0082] (2) Take 1, 2, 3, 4, 5, 6, and 7 mL of 20 μg / mL standard Cl - The solution was placed in a 25 mL volumetric flask, and 2 mL of HNO3, 2 mL of acetone, and 1 mL of 5 mg / mL AgNO3 were added, followed by dilution with deionized water and shaking.

[0083] (3) Same as NO3 - Step (2) of the content determination method: In a clean 10 mL test tube, add 0.8 mL HNO3, 0.8 mL acetone, 0.4 mL AgNO3, 0.8 mL plant extract and 7.2 mL deionized water, shake well, and place at room temperature for 15 minutes. Use water as a blank control. Other conditions are the same as those of the experimental group.

[0084] (4) Reference method (Zhou Qiang, Li Ping, Cao Jinhua, Yu Bingjun. Comparison of titration and spectrophotometry for determining chloride ion content in plants [J]. Plant Physiology Communications, 2007, (06): 1163-1166.). The absorbance value was measured at 335 nm using an ultraviolet spectrophotometer, and a standard curve was drawn. The Cl content of each sample was calculated according to the regression equation. - content.

[0085] PO4 in plants 3- Content determination method: Molybdenum blue method for determination of phosphorus content

[0086] (1) Prepare 50ug / mL standard phosphorus solution. Refer to the method (Liu Jie. Comparison of the adaptation mechanism of sunflower to alkaline stress and salt stress [D]. Northeast Normal University, 2011.), accurately weigh 0.0439g KH2PO4, dissolve it in 100mL deionized water, add 5mL (concentrated) H2SO4, and dilute to 250mL; prepare ammonium molybdate-sulfuric acid mixed solution, weigh 10g ammonium molybdate, and dissolve it in 80mL deionized water. Slowly pour 112mL (concentrated) H2SO4 into 160mL deionized water and cool. Mix the two solutions and dilute to 400mL with deionized water; prepare 0.1mol / LSnCl2 solution, weigh 0.95g SnCl2, add 10mL (concentrated) HCl, and dilute to 50mL.

[0087] (2) According to Table 1, determine the OD value at each phosphorus concentration and draw a standard curve: mix and let stand for 10-15 minutes, and measure the OD value of each standard solution at a wavelength of 660nm.

[0088] (3) Determination of phosphorus content in plant tissue fluid: Take 0.3g of leaves, add 5mL of deionized water, grind thoroughly, centrifuge at 3000g for 15min, and keep the supernatant. If coloration occurs, decolorize with activated carbon. Take two 1mL portions of tissue fluid and place them in 5mL test tubes respectively, and determine their OD values ​​under the same conditions as above.

[0089] Content of each component in standard phosphorus solution of different concentrations

[0090] Phosphorus concentration (μg / mL) 0 10 15 20 30 35 40 50 Standard phosphorus solution (mL) 0 0.2 0.3 0.4 0.6 0.7 0.8 1 Distilled water (mL) 1 0.8 0.7 0.6 0.4 0.3 0.2 0 Ammonium molybdate-sulfuric acid mixture (mL) 3 3 3 3 3 3 3 3 <![CDATA[SnCl2(mL)]]> 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1

[0091] (4) Calculation method: phosphate mass concentration P (ug / gFw) = ρ·v / m; ρ is the phosphorus content of the extract (ug / mL); v is the volume of the extract (mL); m is the fresh weight of the sample (g).

[0092] Determination of photosynthetic parameters in plant leaves

[0093] According to the reference method (Meng Guohua. Effects of different nitrogen, phosphorus and potassium levels on the growth of sweet sorghum under salt stress [D]. Shandong Normal University, 2012.), mature leaves of the same growth and leaf position of the ice leaf daylily seedlings were selected to measure their photosynthetic parameters. The measurement time was from 10:00 to 12:00 in the morning and from 2 to 4 pm. Each photosynthetic parameter (Pn, Ci, Tr, Gs) in each treatment was measured with 15 replicates.

[0094] Example 1 Nitrogen Treatment

[0095] Seedlings of the same size and growth were treated with 0mM, 100mM and 200mM NaCl. Each group of salt treatments was irrigated with 5 different concentrations of NO3, namely 0.1, 2.5, 5, 7.5 and 10mM. -Nutrient solution (other nutrients are the same as standard Hoagland nutrient solution, see Table 1). Five replicates were set for each treatment, and various physiological indices were measured after 28 days of treatment.

[0096] Table 1 Different concentrations of NO3 - The content of each component in the nutrient solution (mM), the content of trace elements is the same as the standard Hoagland nutrient solution

[0097] Added nutrients <![CDATA[0.1NO3 - ]]> <![CDATA[2.5NO3 - ]]> <![CDATA[5NO3 - ]]> <![CDATA[7.5NO3 - ]]> <![CDATA[10NO3 - ]]> <![CDATA[Ca(NO3)2]]> 0.05 1.25 2.5 3.75 5 <![CDATA[MgSO4]]> 2 2 2 2 2 <![CDATA[KH2PO4]]> 1 1 1 1 1 <![CDATA[K2SO4]]> 2.5 2.5 2.5 2.5 2.5 <![CDATA[CaCl2]]> 5 3.75 2.5 1.25 0

[0098] Example 2 Phosphorus Treatment

[0099] Other conditions were the same as those of the nitrogen treatment. The phosphorus treatment was to irrigate each group with 5 different concentrations of PO4: 0.1, 0.5, 1, 1.5, and 2 mM. 3- Nutrient solution (other nutrients are the same as standard Hoagland nutrient solution, see Table 2). Five replicates were set for each treatment, and various physiological indicators were measured after 28 days of treatment.

[0100] Table 2 Different concentrations of PO4 3- The content of each component in the nutrient solution (mM), the content of trace elements is the same as the standard Hoagland nutrient solution

[0101]

[0102]

[0103] Example 3 Potassium treatment

[0104] Other conditions were the same as those of the nitrogen treatment. The potassium treatment was to irrigate each group with 5 different concentrations of K: 0.1, 2, 4, 8, and 10 mM. + Nutrient solution (other nutrients are the same as standard Hoagland nutrient solution, see Table 3). Five replicates were set for each treatment, and various physiological indicators were measured after 28 days of treatment.

[0105] Table 3 Different concentrations of K + The content of each component in the nutrient solution (mM), the content of trace elements is the same as the standard Hoagland nutrient solution

[0106] Added nutrients <![CDATA[0.1K + ]]> <![CDATA[2K + ]]> <![CDATA[4K + ]]> <![CDATA[8K + ]]> <![CDATA[10K +- ]]> <![CDATA[Ca(NO3)2]]> 5 5 5 5 5 <![CDATA[MgSO4]]> 2 2 2 2 2 <![CDATA[NaH2PO4]]> 1 1 1 1 1 <![CDATA[NaNO3]]> 5 5 5 5 5 KCl 0.1 2 4 8 10

[0107] Example 4 Calcium Treatment

[0108] Other conditions were the same as those of the nitrogen treatment. The calcium treatment was to irrigate each group with 5 different concentrations of Ca: 0.1, 2, 5, 10, and 15 mM. 2+ Nutrient solution (other nutrients are the same as standard Hoagland nutrient solution, see Table 5). Five replicates were set for each treatment, and various physiological indicators were measured after 28 days of treatment.

[0109] Table 4 Ca concentrations at the same level 2+The content of each component in the nutrient solution (mM), the content of trace elements is the same as the standard Hoagland nutrient solution

[0110] Added nutrients <![CDATA[0.1Ca 2+ ]]> <![CDATA[2Ca 2+ ]]> <![CDATA[5Ca 2+ ]]> <![CDATA[10Ca 2+ ]]> <![CDATA[15Ca 2+ ]]> <![CDATA[NaNO3]]> 10 10 10 10 10 <![CDATA[KNO3]]> 5 5 5 5 5 <![CDATA[MgSO4]]> 2 2 2 2 2 <![CDATA[KH2PO4]]> 1 1 1 1 1 <![CDATA[CaCl2]]> 0.1 2 5 10 15

[0111] Example 5

[0112] Seedlings of the same size and growth vigor were treated with 0 mM, 100 mM and 200 mM NaCl respectively. Under the premise of each NaCl concentration treatment, the four-factor three-level method was used to carry out orthogonal design experiments. The four factors refer to nitrogen (A), phosphorus (B), potassium (C), and calcium (D). Each factor was set at three levels. The three levels of nitrogen, phosphorus, potassium, and calcium were A1A2A3, B1B2B3, C1C2C3, and D1D2D3, respectively. Among them, A2, B2, C2, and D2 represent the optimal nitrogen level (7.5mM), optimal phosphorus level (1mM), optimal potassium level (4mM), and optimal calcium level (5mM) required for seedling growth, respectively; A1, B1, C1, and D1 were lower than the optimal levels of each element, which were 5, 0.5, 2, and 2mM, respectively; A3, B3, C3, and D3 were higher than the optimal levels of each element, which were 10, 1.5, 8, and 10mM, respectively, as shown in Table 6. A total of 9 treatments were designed, with 5 replications for each treatment. The three levels of each factor and the content of each element in each treatment are shown in Table 5.

[0113] Table 4 Four-factor three-level orthogonal test table of each nutrient component

[0114] deal with combination deal with combination deal with combination T1 A1B1C1D1 T4 A2B1C2D3 T7 A3B1C3D2 T2 A1B2C2D2 T5 A2B2C3D1 T8 A3B2C1D3 T3 A1B3C3D3 T6 A2B3C1D2 T9 A3B3C2D1

[0115] Table 5 Contents of various components in nutrient solutions with different ratios of nitrogen, phosphorus, potassium and calcium (mM), T0 watering standard Hogland nutrient solution

[0116]

[0117]

[0118] Example 6

[0119] Other conditions were the same as the nitrogen treatment, and in this embodiment, each group was irrigated with different solutions in Table 6. Five replicates were set for each treatment, and various physiological indicators were measured after 28 days of treatment.

[0120] Table 6 Content of each component in different solutions (mM)

[0121]

[0122] The test results of the above embodiments 1-6 are analyzed as follows:

[0123] It can be seen that 1mM NO3 -Compared with the treatments, 2.5, 5, 7.5 and 10 mM NO3 - All treatments significantly promoted the growth of P. iceleaf seedlings, as shown by a significant increase in biomass and photosynthesis; Na + and Cl - The content of K + and NO3 - In the 0mM and 100mM NaCl treatment groups, 7.5mM and 10mM NO3 - is the most suitable concentration for the growth of ice leaf daylily seedlings, while in the 200mM NaCl treatment group, 5mM and 7.5mM NO3 - is the most suitable, indicating that the appropriate amount of NO3 - It can effectively alleviate the damage caused by salt stress to ice leaf daylily. In short, under the optimal and stress NaCl treatment, 7.5mM NO3 - It is the optimal concentration for the growth of ice leaf daylily seedlings.

[0124] The biomass, chlorophyll content and photosynthesis of the ice leaf daylily seedlings increased first and then decreased; the Na + and Cl - The content first decreased and then increased, K + The change trend of PO4 is opposite to that of 3- In the 0mM and 100mM NaCl treatment groups, compared with 0.1mM PO4 3- Compared with 0.5 and 1mM PO4 3- is the most suitable phosphorus level for promoting the growth of ice leaf daylily seedlings. + and Cl- contents were significantly lower than those in other treatment groups, and K + The highest content and the strongest photosynthesis. In the 200mM NaCl treatment group, 1 and 1.5mM PO4 3 - is the most suitable. In summary, under the optimal and stress NaCl treatments, 1mM PO4 3- It is the optimal concentration for the growth of ice leaf daylily seedlings.

[0125] With K + With the increase of Na concentration, the biomass and photosynthesis of the ice leaf daylily seedlings first increased and then decreased. + and Cl - The content of NO3 decreased first and then increased. - The trend of K + The content continued to increase. Regardless of the NaCl concentration, with 0.1mM K + In comparison, the application of 4mM and 8mM K +When the seedlings are grown, the biomass is the largest, the chlorophyll content is the highest, the photosynthesis is the strongest, and the Na + and Cl - The lowest content, NO3 - Therefore, 4mM and 8mM K + It is the optimal concentration for seedling growth and can effectively improve the salt tolerance of Ice Leaf Daylily.

[0126] Under 0 and 100 mM NaCl treatments, the three nitrogen, phosphorus, potassium and calcium ratios of T4 (A2B1C2D3; N:P:K:Ca=7.5:0.5:4:10), T5 (A2B2C3D1; N:P:K:Ca=7.5:1:8:2) and T7 (A3B1C3D2; N:P:K:Ca=10:0.5:8:5) significantly increased the biomass and photosynthesis of P. iceleaf seedlings, and significantly reduced Na + and Cl - The content of K + 、NO3 - and PO4 3- In the 200mM NaCl treatment group, the biomass of the seedlings was the largest and the photosynthesis was the strongest when the two nitrogen, phosphorus, potassium and calcium ratios were T5 (A2B2C3D1; N:P:K:Ca=7.5:1:8:2) and T7 (A3B1C3D2; N:P:K:Ca=10:0.5:8:5). + and Cl - The content of K + 、NO3 - and PO4 3- Therefore, no matter what NaCl concentration is used, the nutrient solution with a nitrogen, phosphorus, potassium and calcium ratio of 7.5:1:8:2 (T5) or 10:0.5:8:5 (T7) can significantly promote the growth of ice leaf daylily, which is the most suitable nitrogen, phosphorus, potassium and calcium ratio required for seedling growth and can improve the seedling's ability to tolerate salt stress.

[0127] Study on the influence of different salt treatments on the content of active ingredients in Ice Leaf Daylily: Ice Leaf Daylily has α-glucosidase inhibitory activity and antioxidant activity. This group of experiments referred to the method of the paper "Study on the Chemical Components of Ice Plant" to study the relationship between the content of dibutyl phthalate and cyclo(phenylalanine-valerian) dipeptide in the ethanol extract of Ice Plant under NaCl stress treatment and salt. The results are as follows:

[0128] Table 6 Effect of various salts on the content of dibutyl phthalate and cyclo(phenylalanine-valerian) dipeptide

[0129]

[0130] CK was treated with NaCl stress only.

[0131] As shown in Table 6, in this application, under the stress NaCl treatment, NO3-, PO4 3- , K + , Ca 2+ The contents of dibutyl phthalate and cyclo(phenylalanine-valerian) dipeptide, which have α-glucosidase inhibitory and antioxidant activities, in ice leaf helianthus annuus were studied. The results showed that different salts (NO3 - PO4 3- , K + , Ca 2+ ) had different effects on the content of dibutyl phthalate in ice leaf daylily, among which PO4 3- , Ca 2+ The content of dibutyl phthalate in ice leaf daylily can be effectively increased. Further, the present application also attempts to add PO4 3- , Ca 2+ After combined processing, it can be seen that PO4 3- , Ca 2+ The combination can play a synergistic role and significantly increase the content of dibutyl phthalate in ice leaf sunflower, so as to obtain more active substances with α-glucosidase inhibitory activity and antioxidant activity, thereby improving its economic value.

[0132] On the other hand, different salts (NO3 - PO4 3- , K + , Ca 2+ ) also had different effects on the content of cyclic (phenylalanine-valerian) dipeptide in P. iceleaf. Among them, PO4 3- It can effectively increase the content of cyclic (phenylalanine-valerian) dipeptide in ice leaf daylily. Further, the present application also attempts to add PO4 3- , K + After combined processing, it can be seen that PO4 3- , K + The combination of can also increase the content of cyclic (phenylalanine-valerian) dipeptide in ice leaf daylily.

[0133] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. NO 3- The application of the method for improving the biomass, chlorophyll, photosynthetic parameters and regulating ion content of Mesembryanthemum glaucoma seedlings under salt stress is characterized in that: The specific conditions of the salt stress are: the concentration of NaCl is 100mM or 200mM; NO 3- The concentration is 2.5~10mM.

2. NO as claimed in claim 1 3- The application of the method for improving the biomass, chlorophyll, photosynthetic parameters and regulating ion content of Mesembryanthemum glaucoma seedlings under salt stress is characterized in that: The method for adjusting the ion content comprises: reducing the Na + and Cl - content.

3. NO as claimed in claim 1 3- The application of the method for improving the biomass, chlorophyll, photosynthetic parameters and regulating ion content of Mesembryanthemum glaucoma seedlings under salt stress is characterized in that: The adjusting ion content comprises: increasing the K content in the ice leaf daylily + and NO3 - content. 4.PO4 3- The application of the method for improving the biomass, chlorophyll, photosynthetic parameters and regulating ion content of Mesembryanthemum glaucoma seedlings under salt stress is characterized in that: The specific conditions of the salt stress are: the concentration of NaCl is 100mM or 200mM; The PO4 3- The concentration is 0.5~1mM.

5. PO4 as claimed in claim 4 3- The application of the method for improving the biomass, chlorophyll, photosynthetic parameters and regulating ion content of Mesembryanthemum glaucoma seedlings under salt stress is characterized in that: The method for adjusting the ion content comprises: reducing the Na + and Cl - content.

6. PO4 as claimed in claim 4 3- The application of the method for improving the biomass, chlorophyll, photosynthetic parameters and regulating ion content of Mesembryanthemum glaucoma seedlings under salt stress is characterized in that: The adjusting ion content comprises: increasing the K content in the ice leaf daylily + and PO4 3- content. 7.K + The application of the method for improving the biomass, chlorophyll, photosynthetic parameters and regulating ion content of Mesembryanthemum glaucoma seedlings under salt stress is characterized in that: The specific conditions of the salt stress are: the concentration of NaCl is 100mM or 200mM; The K + The concentration is 4-8mM.

8. The method according to claim 7 + The application of the method for improving the biomass, chlorophyll, photosynthetic parameters and regulating ion content of Mesembryanthemum glaucoma seedlings under salt stress is characterized in that: The method for adjusting the ion content comprises: reducing the Na + and Cl - content; Or, the adjusting ion content comprises: increasing the K + and NO3 - content.

9. Application of nutrient solution T5 or T7 in increasing biomass, chlorophyll, photosynthetic parameters and regulating ion content of Mesona glaucosa seedlings under salt stress, characterized in that: The nutrient solution T5 contains NO 3- PO4 3- , K + , Ca 2+ , where the concentration ratio of N:P:K:Ca is 7.5:1:8:2; The nutrient solution T7 contains NO 3- PO4 3- , K + , Ca 2+ , wherein the concentration ratio of N:P:K:Ca is 10:0.5:8:5; Or, the adjusting ion content comprises: reducing the Na + and Cl - content; Or, the adjusting ion content comprises: increasing the K + and PO4 3- content.

10. Use PO4 3- , Ca 2+ Application of combined treatment in increasing the content of dibutyl phthalate in Ice-leaved Helianthus annuus.

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