Non-blood serum plant protein peptide universal cell culture medium

Abstract

A serum-free vegetable protein peptide general cell culture medium relates to a serum-free or low-serum general cell culture medium. The serum-free vegetable protein peptide general cell culture medium solves the problems that the existing serum-free culture medium can not directly culture the cells with adverse growth status, most of cells can be adaptable to the existing serum-free culture medium after the gradual reduction of the serum concentration, and the existing serum-free culture medium is vulnerable to the external factors and easy to cause fatal damage. The serum-free vegetable protein peptide general cell culture medium is prepared by calcium chloride, potassium chloride, magnesium sulfate anhydrous, sodium chloride, anhydrous sodium dihydrogen phosphate, L-glutamine, D-glucose, phenol red, D-calcium pantothenate, choline chloride, folic acid, i-inositol, nicotinamide, pyridoxal hydrochloride, riboflavin, thiamine hydrochloride, soybean protein peptide and distilled water. Cells can be directly cultured on the culture medium of the invention without gradual habituation and culture, thus shortening culture period and avoiding the interference by other factors, reducing the virus pollution which is caused by serum; the cell survival rate reaches more than 94 percent.

Description

technical field [0001] The present invention relates to serum-free or low-serum universal cell culture media. Background technique [0002] Cells are the expression vectors of viruses and proteins, and the quality of cells directly affects the expression level. Only a good medium can screen and domesticate high-quality cells. Ordinary cell culture medium contains calf serum. Because serum is produced in batches, the quality varies greatly among batches and the quality is unstable. Moreover, serum contains some substances that are toxic to cells, which will affect the growth of cells and even cause cell death. At the same time, it is more difficult to separate and purify in industrial production; and it is especially unsuitable for the study of the effect of growth factors on cells, the study of the function of cell secretions, and the preparation of a large number of cell secretions. [0003] Serum-free medium is a synthetic medium that can maintain the growth and reproduct...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the existing serum-free medium cannot directly cultivate cells with poor growth conditions. Most cells must gradually reduce the serum concentration to adapt to the existing serum-free medium and are easily affected by external factors and easily cause lethal damage. problem, while providing a serum-free vegetable protein peptide universal cell culture medium

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