Liquid phase chip reagent kit detecting various anti EB viral antigen antibody

An Epstein-Barr virus, antigen-antibody technology, applied in the field of medicine and biology, to achieve the effect of large flux, small sample volume, and elimination of subjectivity

Active Publication Date: 2007-10-31
SUN YAT SEN UNIV CANCER CENT
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AI Technical Summary

Problems solved by technology

At present, there is no liquid phase chip kit for early diagnosis of nasopharyngeal carcinoma at home and abroad

Method used

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Examples

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Embodiment Construction

[0021] The natural or genetically engineered antigens used in this example can be purchased. Among them, the capsid antigen gp125 in the EB antigen is obtained through affinity chromatography purification of monoclonal antibody and P3H3 cell lysate, and the capsid antigen p18 (1-119aa), namely NO.35, p23 in the series table (that is, NO.36 in the series table), NO.37 in the nuclear antigen EBNA-1 full-length series table and nuclear antigen EBNA-1 (1-90, 408-498aa) ( That is, NO.34 in the series table), EA-D (EBV early antigen D (EA-D) recombinant, NO.33 in the series table) was purified after expression in Escherichia coli, and the EB virus total antigen was obtained from P3H3 cell lysate was obtained, and 32 peptides were also synthesized, including 2 alkaline DNase, 2 thymokinase, 2 DNA polymerase, 2 Zebra (BZLF1), Gp350 / 220 (BLLF1), P54 / 47 (BMRF1), EBNA1(BKRF1), gp110 / gp125(BALF4), P18(BFRF3), gN(BLRF1), EBNA2(BYRF1), EBNA-3a(BLRF3), EBNA-3c(BERF3), EBNA-LP, ribose Nuclei...

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Abstract

This invention discloses one liquid phase chip test agent case and its process method for multiple kinds of anti-EB virus antibody, which comprises the following parts: coupling affinity element multi-color microball covering on polypeptide; at least one kind of coupling hydroxyl group multi-color microball covering on natural or gene antigen, wherein the antigen is EB virus crack object or shell antigen VCA; gene antigen is of one amino acid in list of No. 33-No. 37; the said integration polypeptide is one from No. 1-No. 32.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and in particular relates to a liquid-phase chip kit and a preparation method for detecting various anti-EB virus antigen antibodies. Background technique [0002] Nasopharyngeal carcinoma is a malignant tumor prevalent in southern my country, and Guangdong is the most common, with an annual incidence of 30-50 / 100,000. The 5-year survival rate of early nasopharyngeal carcinoma after radiotherapy can reach 80-90%; while that of advanced nasopharyngeal carcinoma is only 20%. Therefore, strengthening the secondary prevention of nasopharyngeal carcinoma, that is, early detection, early diagnosis and early treatment is the key to improving the therapeutic effect of nasopharyngeal carcinoma. [0003] The occurrence of nasopharyngeal carcinoma is closely related to Epstein-Barr virus infection. Although more than 90% of the general population have established a lifelong latent infection o...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/543G01N33/531
Inventor 曾益新谷爱娣谢彦博
Owner SUN YAT SEN UNIV CANCER CENT
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