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Probiotics composition with mammary gland hyperplasia regulating function and application of probiotics composition

A breast hyperplasia and probiotic technology, applied in the field of biomedicine, can solve the problems of slow effect and long cycle, and achieve the effects of improving pelvic inflammation, promoting the secretion of luteinizing hormone, and inhibiting the synthesis of collagen fibers.

Active Publication Date: 2017-06-06
泰安大凡神农生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional Chinese medicine treatment generally requires a long period of time, and the effect is slow
However, there is no report on the effect of probiotics on the treatment of mammary gland hyperplasia

Method used

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  • Probiotics composition with mammary gland hyperplasia regulating function and application of probiotics composition
  • Probiotics composition with mammary gland hyperplasia regulating function and application of probiotics composition
  • Probiotics composition with mammary gland hyperplasia regulating function and application of probiotics composition

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Experimental program
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Effect test

preparation example Construction

[0054] The preparation method of above-mentioned probiotic composition, the steps are as follows:

[0055] (1) Ferment and cultivate Lactobacillus plantarum, Lactobacillus acidophilus and Bifidobacterium animal respectively, so that the concentration of bacteria in the fermentation broth reaches the logarithmic growth phase of 0.5-2.0×10 9 cfu / ml;

[0056] (2) centrifuging the fermented liquid respectively, discarding the supernatant to obtain the sludge of Lactobacillus plantarum, Lactobacillus acidophilus and Bifidobacterium animalis;

[0057] (3) Mix the fungus slime and the freeze-drying protective agent evenly, and freeze-dry to obtain the product.

[0058] The strain protection agent can be selected from common strain protection agents in the art.

[0059] In a specific embodiment, the formula of the lyoprotectant is: 8 g of sucrose, 26 g of skimmed milk powder, 1 mL of glycerin, 100 mL of distilled water, sterilized at 115° C. for 15 minutes.

[0060] Preferably, the...

Embodiment 1

[0078] Embodiment 1: the preparation of the lyophilized powder of probiotics

[0079] 1) Lactobacillus plantarum (preservation number is CCTCC M2010150), Lactobacillus acidophilus (preservation number is CCTCC NO: M2015129), and bifidobacterium animalis are respectively activated in the culture medium, inoculated in the fermentation medium, shaken and cultured, so that Bacteria concentration reaches logarithmic growth phase 0.5-2×10 9 CFU / mL.

[0080] Preparation of Lactobacillus seed solution: Take out the strain preservation tube, draw a plate with MRS solid medium for recovery, and culture anaerobically at 37°C for 48 hours. Pick a single colony under the plate and inoculate it into 100 ml of MRS medium, and culture it anaerobically in a 37°C incubator for 24 hours. The seeds are inoculated into a 3L large triangular flask with a 5% inoculum amount (the liquid volume is 2.5L), cultured anaerobically at 37°C for 24-48 hours, and the concentration of the bacteria solution i...

Embodiment 2

[0088] Embodiment 2: the preparation of Chinese medicine extract

[0089] 1) By weight: ginseng 6g, Poria cocos 12g, kombu 12g, tangerine peel 10g;

[0090] 2) Soak the mixture in 10 times the water for 50 minutes, decoct, boil the water for 40 minutes, and filter to obtain the first filtrate; decoct the dregs with 8 times the water for the second time, boil the water for 40 minutes, filter The obtained second filtrate is mixed evenly with the first filtrate, and the traditional Chinese medicine liquid is concentrated by a vacuum decompression rotary evaporator until the solid content is more than 30%, and then enters the spray dryer, the inlet air temperature is controlled at 140°C, and the outlet air temperature is 90°C. The rotation speed of the atomizer is 45 rpm, and the feed rate is 5ml / min to obtain a spray-dried powder, which is pulverized and passed through a sieve of more than 100 meshes to obtain the Chinese medicine extract.

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Abstract

The invention discloses a probiotics composition with a mammary gland hyperplasia regulating function. The probiotics composition is prepared from lactobacillus plantarum, lactobacillus acidophilus and bifidobacterium animalis. In the probiotics composition, the content of the lactobacillus plantarum is 5*10<6> to 5*10<9> CFU / g, the content of the lactobacillus acidophilus is 2.5*10<6> to 2.5*10<9> CFU / g, and the content of the bifidobacterium animalis is 2.5*10<6> to 2.5*10<9> CFU / g. The probiotics composition is further cooperated with traditional Chinese medicine extracts to prepare a probiotics traditional Chinese medicine preparation. Furthermore, by combination of components such as the probiotics composition, the traditional Chinese medicine extracts and prebiotics, a product with the mammary gland hyperplasia regulating function can be prepared. The probiotics composition, the probiotics traditional Chinese medicine preparation and the product can promote multiplication of probiotics in a human body and recover balance of female hormones and progestational hormones, further can adjust the intestinal flora of the human body while relieving mammary gland hyperplasia, and is beneficial for the health of the human body.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a probiotic composition with the function of regulating mammary gland hyperplasia and its application. Background technique [0002] According to the latest survey of the World Health Organization, more than 80% of women in the world suffer from breast disease to a certain degree. Breast cancer has developed into the malignant tumor with the highest incidence rate in the world. About 1.2 million women in the world suffer from breast cancer every year, and 50 Ten thousand people die from breast cancer, and the fatality rate exceeds 41%. Since the 1990s, the incidence rate of breast cancer in China has increased more than twice that of the world, especially in urban areas. The number of breast cancer detections in China is half of that in Europe and basically equal to that in the United States. If this trend remains unchanged, by 2021, there will be as many as 2.5 million breast cancer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61K36/752A61P15/14A61P15/00A61P1/00A61K35/745A61K31/702
CPCA61K31/702A61K35/745A61K35/747A61K36/03A61K36/076A61K36/258A61K36/752A61K2236/331A61K2236/39A61K2236/51A61K2300/00
Inventor 单宝龙张建梅杨婷婷樊梅娜陈甜甜房栋张智磊刘红程秀芳孙尹双
Owner 泰安大凡神农生物有限公司
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