Method for inactivating viruses in protein solution by adopting ultraviolet irradiation

A technology of protein solution and ultraviolet rays, which is applied in the direction of irradiation, disinfection, sanitary equipment for toilets, etc., can solve the problem of destruction of effective protein components and achieve good inactivation effect

Active Publication Date: 2017-12-22
广州白云山拜迪生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the existing method for inactivating viruses in protein solutions by ultraviolet irradiation often destroys effective protein components in protein solutions while inactivating viruses

Method used

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  • Method for inactivating viruses in protein solution by adopting ultraviolet irradiation
  • Method for inactivating viruses in protein solution by adopting ultraviolet irradiation
  • Method for inactivating viruses in protein solution by adopting ultraviolet irradiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Flow type protein solution ultraviolet inactivation instrument

[0046] like Figure 1 ~ Figure 3 As shown, the flow type protein solution ultraviolet inactivation instrument used in the present invention comprises a cylinder 1, the cylinder is a dark box, the inside of the cylinder is coated with a reflective coating 2, and the side of the cylinder is provided with an openable movable panel 10 , and the movable panel is provided with a visual window, which is convenient for observing the situation in the cylinder, and through this window, the probe of the ultraviolet intensity meter can be inserted into the cylinder to measure the intensity of ultraviolet rays, or the thermometer can be inserted into the cylinder to measure the temperature.

[0047] Two sets of UV lamp groups 3 arranged symmetrically up and down are fixed in the cylinder body 1. The purpose of symmetrical placement is to ensure that the protein solution can be irradiated with ultraviolet ray...

Embodiment 2

[0053] Example 2: Flow type protein solution ultraviolet inactivation instrument

[0054] like Figure 4~5 As shown, in the flow-type protein solution ultraviolet inactivation instrument of this embodiment, the protein solution flow tube 5 is in a spiral shape, and what is selected is a quartz glass tube. The input end of the protein solution flow pipe 5 passes through the lower end of the side panel of the cylinder body 1 and is arranged outside the cylinder body, and the output end of the protein solution flow pipe 5 passes through the upper end of the side panel of the cylinder body 1 and is arranged outside the cylinder body, and the protein solution flows The input end and output end of the tube 5 are respectively located at the two ends of the barrel. The structure of other parts of the flow-type protein solution ultraviolet inactivation instrument of this embodiment is the same as that of Embodiment 1.

[0055] In other embodiments, the protein solution flow pipe can ...

Embodiment 3

[0056] Example 3: Inactivation verification of porcine parvovirus (PPV) in fibrinogen

[0057] The cultivation of porcine parvovirus (PPV): add the porcine parvovirus (PPV) liquid into a monolayer or overgrow 90% porcine testicular cells (ST cells, Image 6 ), after 2 hours of adsorption, discard the virus liquid, add DMEM medium containing 2% fetal bovine serum (FBS) to culture the virus, and collect the virus liquid when 3 / 4 cells have lesions after about 72 hours. The culture medium containing the virus and host cells was placed at -20°C and repeatedly frozen and thawed 3 times to break up the host cells and release the virus. Then, centrifuge at 4° C. (3000 rpm, 10 min) to remove cell debris, and the supernatant is the desired virus suspension.

[0058] Determination of virus titer: 10-fold serial dilution of the virus stock solution was performed with DMEM culture solution containing 2% FBS. Take a 96-well cell culture plate covered with a monolayer of host cells, pour ...

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Abstract

The invention discloses a method for inactivating viruses in a protein solution by adopting ultraviolet irradiation. The method is characterized by comprising the following steps: S1, preparing the protein solution to be treated in a sealed container; S2, connecting the sealed container with a flow type protein solution ultraviolet inactivating instrument; and S3, switching on an ultraviolet lamp set for irradiating the protein solution, wherein the intensity for irradiating a protein solution flowing tube of ultraviolet rays is 2.0-10.0 mw / cm<2>, the irradiation time is 5s-60min, injecting the protein solution into the flowing tube, and after irradiation, collecting the protein solution with the viruses inactivated. According to the method provided by the invention, the protein solution is sufficiently mixed in a specific device, the ultraviolet rays can carry out inactivation on the microbes including viruses, bacteria and the like, while the inactivating effect is achieved, the active ingredients of protein in the protein solution are also reserved.

Description

technical field [0001] The invention relates to a method for inactivating virus in flowing liquid, in particular to a method for inactivating virus in protein solution by ultraviolet irradiation. Background technique [0002] In the production process of protein solutions, the uncertainty of production safety is often increased due to the use of materials derived from human or animal tissues or body fluids. Therefore, in order to improve the safety of process production and to avoid the threat of pathogens in the process of using the product, product safety must be fully considered during the research and development of protein solutions. my country's "Blood Products Removal / Inactivation Virus Technical Methods and Verification Guidelines" pointed out: In order to improve the safety of blood products, the production process must have a certain ability to remove / inactivate some viruses, and there should be specific removal / inactivation in the production process. virus method....

Claims

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Application Information

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IPC IPC(8): A61L2/10A61L2/26
CPCA61L2/0047A61L2/26A61L2202/21
Inventor 张俊辉陈柯君朱晋辉万华印
Owner 广州白云山拜迪生物医药有限公司
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