Cell-factory-based measles virus stock solution and measles-series attenuated live vaccine preparation

Abstract

The invention provides a cell-factory-based measles virus stock solution and a measles-series attenuated live vaccine preparation. A preparation method of the measles virus stock solution includes selecting SPF chick-embryo cells, and adding cell culture fluid to prepare cell suspension liquid, and adding the cell suspension liquid into a cell factory; inoculating the cell factory with working seeds of measles viruses and the cell suspension liquid according to the ratio of the working seeds to the cell suspension liquid being (0.005-0.05):1, and standing and cultivating the cell factory at the temperature of 33+/-1 DEG C for 3-4 days, pouring out archeocyte culture fluid, and replacing with fresh cell growth liquid for continuous cultivation; during cytopathy, collecting single measles virus liquid step by step. In an equal production scale, the batch number of cell dissociation is reduced, and the high-titer measles virus liquid is obtained, so that quality uniformity of measles-series vaccine products is improved effectively, and product yield is increased.

Description

technical field [0001] The invention belongs to the technical field of virus vaccines, and relates to a cell factory for preparing measles virus stock solution and measles series live attenuated vaccine preparations. Background technique [0002] Measles is caused by the measles virus and is an acute infectious disease transmitted through the respiratory tract. The susceptible population includes all children and adults with no specific immunity and low immunity. The morbidity and fatality rate of measles ranked first among childhood infectious diseases before the use of vaccines. At present, vaccination is still the most effective measure to prevent measles infection and control its epidemic. [0003] At this stage, the culture of measles virus at home and abroad mostly uses the primary chicken embryo cell spinner bottle culture technology. The spinner bottle technology is a traditional adherent cell culture technology. During the process, the spinner bottle is continuous...

AI Technical Summary

Problems solved by technology

[0004] (1) The degree of automation is low, and the workload of manual operation is large. Although there is a bottle turning machine to assist, a large number of manual operations are still required in the operation of each process, and automation and standardization cannot be realized;

[0005] (2) A large number of cleaning verification work, the cleaning quality is affected by human factors, it is difficult to ensure the consistency of the cleaning effect, and the cost of labor and cleaning verification is increasing year by year;

[0006] (3) Occupying a lot of space, under the existing conditions, the limitations of the spin bottle itself are difficult to industrialize or scale production;

[0007] (4) The manual operation is cumbersome and the glass is fragile, which increases the risk of personnel infection and the pollution risk of the operating environment significantly increases;

[0008] (5) The controllability of the production process is poor, the difference between bottles is large, it is difficult to ensure the consistency of cell batches in product quality, and it takes a long time for cells to grow into a monolayer of fibroblasts, which takes 72-96 hours;

[0009] (6) The virus harvest is a single harvest, and the cells cannot be fully and effectively used, which affects the amplification of production capacity

Theoretically speaking, it is feasible to use bioreactors for primary cell culture, but long-term process exploration is required

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