Plant tissue liquid automatic intermittent immersion circulation culture device

A technology for plant tissue and cyclic culture, which is applied in tissue cell/virus culture devices, biochemical cleaning devices, enzymology/microbiology devices, etc., and can solve problems such as reduced space utilization, high manufacturing costs, and complex RITA structures. , to achieve the effect of reducing labor intensity and reducing dependence

Inactive Publication Date: 2011-12-21
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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Problems solved by technology

The research of Etienne and Berthouly in 2002 showed that the use of semi-automation and BIT can greatly reduce the cost of tissue culture, but the complex structure of RITA

Method used

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  • Plant tissue liquid automatic intermittent immersion circulation culture device
  • Plant tissue liquid automatic intermittent immersion circulation culture device

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Embodiment Construction

[0008] The present invention is described in detail below with reference to accompanying drawing; As shown in the accompanying drawing, structure air pump 1 of the present invention, pressure regulator 2, solenoid valve A3, solenoid valve B3.1, reinforced polyvinyl chloride pipe 4, high temperature resistant High pressure disinfection diversion tube 5, air filter 6, programmable integrated circuit board 7, circuit 8, gas concentration sensor 9, temperature sensor 10, humidity sensor 11, nutrient solution tank 12, culture tank 13, nutrient solution 14 1. Tissue culture seedling 15 is characterized in that at least two groups of culture tanks 13 are arranged around the nutrient solution tank 12, and the nutrient solution tank 12 communicates with the culture tank 13 through a high-temperature and high-pressure sterilization guide tube 5, which is resistant to high temperature and high pressure. One end of the disinfection guide tube 5 extends into the nutrient solution tank 12 an...

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Abstract

The invention relates to a plant tissue liquid automatic intermittent immersion circulation cultivation device, which is characterized in that at least two groups of cultivation tanks are arranged around the nutrient solution tank, and the nutrient solution tank communicates with the cultivation tank through a high-temperature and high-pressure sterilized diversion tube to cultivate Gas concentration sensors, temperature sensors and humidity sensors are installed on the side wall of the tank, solenoid valve A and solenoid valve B are arranged on the upper and lower sides of the programmable integrated circuit board, and the reinforced polychloride on the left side of the programmable integrated circuit board A pressure regulator is set on the vinyl tube, the right side of the air pump is connected to the pressure regulator through a line, the lower end of the air pump is connected to the programmable integrated circuit board through a line, and an air filter is set on the reinforced polyvinyl chloride tube near the nutrient solution tank The front end of the branch pipe on the right side of the reinforced polyvinyl chloride pipe is provided with an air filter. The invention realizes liquid automatic culture, reduces dependence on agar in plant tissue culture, and reduces labor intensity.

Description

technical field [0001] The invention relates to a method and a device for plant tissue liquid culture, in particular to a plant tissue liquid automatic intermittent immersion cycle culture device, which belongs to the technical field of plant tissue culture. Background technique [0002] A variety of bioreactors dedicated to plant tissue culture have appeared in the prior art, such as raft bioreactors, spray bioreactors, and the like. Among them, batch immersion culture is the most widely used, including completely submerged batch culture and semi-submerged batch culture. The earliest intermittent immersion system appeared in 1985, and the plant automatic cultivation system was designed by Tisserat and Vandercook, referred to as the APCS system. The whole system consists of 4 parts: the nutrient solution storage system consists of 2 nutrient reservoirs; the nutrient solution drive system consists of 2 impeller pushers. In the late 1980s, Aitkenl Christie et al. improved th...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/36
Inventor 王永平许建民刘永华李继伟
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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