Plant tissue liquid automatic intermittent immersion circulation culture device

Abstract

The invention relates to a plant tissue liquid automatic intermittent immersion circulation cultivation device, which is characterized in that at least two groups of cultivation tanks are arranged around the nutrient solution tank, and the nutrient solution tank communicates with the cultivation tank through a high-temperature and high-pressure sterilized diversion tube to cultivate Gas concentration sensors, temperature sensors and humidity sensors are installed on the side wall of the tank, solenoid valve A and solenoid valve B are arranged on the upper and lower sides of the programmable integrated circuit board, and the reinforced polychloride on the left side of the programmable integrated circuit board A pressure regulator is set on the vinyl tube, the right side of the air pump is connected to the pressure regulator through a line, the lower end of the air pump is connected to the programmable integrated circuit board through a line, and an air filter is set on the reinforced polyvinyl chloride tube near the nutrient solution tank The front end of the branch pipe on the right side of the reinforced polyvinyl chloride pipe is provided with an air filter. The invention realizes liquid automatic culture, reduces dependence on agar in plant tissue culture, and reduces labor intensity.

Description

technical field [0001] The invention relates to a method and a device for plant tissue liquid culture, in particular to a plant tissue liquid automatic intermittent immersion cycle culture device, which belongs to the technical field of plant tissue culture. Background technique [0002] A variety of bioreactors dedicated to plant tissue culture have appeared in the prior art, such as raft bioreactors, spray bioreactors, and the like. Among them, batch immersion culture is the most widely used, including completely submerged batch culture and semi-submerged batch culture. The earliest intermittent immersion system appeared in 1985, and the plant automatic cultivation system was designed by Tisserat and Vandercook, referred to as the APCS system. The whole system consists of 4 parts: the nutrient solution storage system consists of 2 nutrient reservoirs; the nutrient solution drive system consists of 2 impeller pushers. In the late 1980s, Aitkenl Christie et al. improved th...

AI Technical Summary

Problems solved by technology

The research of Etienne and Berthouly in 2002 showed that the use of semi-automation and BIT can greatly reduce the cost of tissue culture, but the complex structure of RITA makes the manufacturing cost too high, and the redundant space of BIT culture bottle is too large, which reduces the utilization rate of space. limit their wide application

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