A high-density enrichment method for autotrophic nitrifying bacteria consortia

Abstract

The invention belongs to the field of microbial fermentation, and in particular relates to a high-density enrichment method of an autotrophic nitrifying bacteria consortium and the application of the obtained high-density autotrophic nitrifying bacteria consortium to sewage treatment. The bacteria were inoculated in the initial culture medium of autotrophic nitrifying bacteria, at 28°C-32°C, pH 7.5-8.0, dissolved oxygen (DO) controlled at 20%-40%, cultured under agitation conditions, the NO 3 The relationship between ‑N accumulation and the consumption of inorganic carbon sources, the change of pH value during the cultivation process is monitored by pH electrodes, and the addition of inorganic nitrogen sources and inorganic carbon sources to the cultivation system is realized, so that the concentration of ammonia nitrogen is controlled at 150‑ during the cultivation process 300mg / L, and the pH of the system is kept at 7.5-8.0 by adding carbon sources, so as to realize the high-density automatic expansion of autotrophic nitrifying bacteria consortia. The invention effectively solves the problem of poor ammonia nitrogen treatment effect caused by such consortia in the application process of ammonia nitrogen wastewater treatment due to poor strain adaptability, loss of added strains, insufficient number of added strains and high cost.

Description

technical field

[0001] The invention belongs to the field of microbial fermentation, and in particular relates to a high-density enrichment method of an autotrophic nitrifying bacteria consortium and the application of the obtained high-density autotrophic nitrifying bacteria consortium to sewage treatment. Background technique

[0002] Autotrophic nitrifying bacteria are collectively referred to as nitrosifying bacteria and nitrifying bacteria consortia. These microorganisms can convert ammonia nitrogen into nitrite nitrogen and then into nitrate nitrogen, and play an important role in the natural N element cycle. Along with the development of microorganism separation technology, this kind of microorganism is applied in various fields, as patent CN101039692A discloses a kind of consortium composed of Nitrosomonaseutropea and Nitrobacter victoria in aquaculture. Subsequently, this type of consortium was widely used in the treatment of ammonia nitrogen wastewater, but the app...

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