A high-density enrichment method for autotrophic nitrifying bacteria consortia

Abstract

The invention belongs to the field of microbial fermentation, and in particular relates to a high-density enrichment method of an autotrophic nitrifying bacteria consortium and the application of the obtained high-density autotrophic nitrifying bacteria consortium to sewage treatment. The bacteria were inoculated in the initial culture medium of autotrophic nitrifying bacteria, at 28°C-32°C, pH 7.5-8.0, dissolved oxygen (DO) controlled at 20%-40%, cultured under agitation conditions, the NO 3 The relationship between ‑N accumulation and the consumption of inorganic carbon sources, the change of pH value during the cultivation process is monitored by pH electrodes, and the addition of inorganic nitrogen sources and inorganic carbon sources to the cultivation system is realized, so that the concentration of ammonia nitrogen is controlled at 150‑ during the cultivation process 300mg / L, and the pH of the system is kept at 7.5-8.0 by adding carbon sources, so as to realize the high-density automatic expansion of autotrophic nitrifying bacteria consortia. The invention effectively solves the problem of poor ammonia nitrogen treatment effect caused by such consortia in the application process of ammonia nitrogen wastewater treatment due to poor strain adaptability, loss of added strains, insufficient number of added strains and high cost.

Description

technical field

[0001] The invention belongs to the field of microbial fermentation, and in particular relates to a high-density enrichment method of an autotrophic nitrifying bacteria consortium and the application of the obtained high-density autotrophic nitrifying bacteria consortium to sewage treatment. Background technique

[0002] Autotrophic nitrifying bacteria are collectively referred to as nitrosifying bacteria and nitrifying bacteria consortia. These microorganisms can convert ammonia nitrogen into nitrite nitrogen and then into nitrate nitrogen, and play an important role in the natural N element cycle. Along with the development of microorganism separation technology, this kind of microorganism is applied in various fields, as patent CN101039692A discloses a kind of consortium composed of Nitrosomonaseutropea and Nitrobacter victoria in aquaculture. Subsequently, this type of consortium was widely used in the treatment of ammonia nitrogen wastewater, but the app...

Examples

Embodiment 1

[0030] The reaction device includes a fermenter, a carbon source storage tank, a peristaltic pump A, a pH electrode, a control system, a nitrogen source storage tank, a peristaltic pump B, a dissolved oxygen electrode, a temperature probe, a stirring motor, an air compressor, and a heater. The fermenter is inserted with a pH electrode, a dissolved oxygen electrode and a temperature probe, and the pH electrode, the dissolved oxygen electrode and the temperature probe are respectively connected to the control system through wires, and the fermenter is respectively connected to the external mixer, peristaltic pump A, and peristaltic pump B through wires. , the air compressor and the heater are connected, and the peristaltic pump A, peristaltic pump B, air compressor and heater are respectively connected to the control system through wires. In addition, the peristaltic pump A is connected to the carbon source storage tank and the peristaltic pump through pipelines. B passes through...

Embodiment 2

[0038] When Na 2 CO 3 When the cumulative amount X value of the solution consumption reaches V / 4 (L), the expansion is over, and the nitrification rate of the expansion solution is > 160 mg (NO 3 -N) / L / h. Stop stirring and stand still for 1 hour to collect the autotrophic nitrifying bacteria consortium, and the nitrification rate per unit mass of the harvested consortium is >600 mg (NO 3 -N) / g / h. Example 2 100L-scale automatic expansion of nitrosifying bacteria and nitrifying bacteria copolymers

[0039] The volume of the fermenter is 100L, the consortium of nitrosifying bacteria and nitrifying bacteria is used as the inoculum, the inoculum size is 1%, and the initial volume of the medium is V=80L. Stirring speed is 220r / min, keeps constant in the expansion cultivation process, and the inorganic carbon source is 10% Na 2 CO 3 solution, the inorganic nitrogen source is 20% NH 4 Cl solution. During the expansion process, the dissolved oxygen DO was controlled at 20%, and...

Embodiment 3

[0047] Example 3: Automatic enrichment and expansion of 100L-scale activated sludge autotrophic nitrifying bacteria flora

[0048] The volume of the fermenter is 100L, the activated sludge of the pesticide wastewater biochemical pool is used as the inoculum, the inoculum size is 10%, and the initial volume of the medium is V=80L. Stirring speed is 220r / min, keeps constant in the expansion cultivation process, and the inorganic carbon source is 10% Na 2 CO 3 solution, the inorganic nitrogen source is 20% NH 4 Cl solution. During the expansion process, the dissolved oxygen DO was controlled at 20%, and the pH was controlled at 7.50. Among them, the initial medium of autotrophic nitrifying bacteria per liter of Na in water 2 CO 3 2g-4g, MgSO 4 0.2g-0.4g, KCl 0.2g-0.4g, phosphoric acid to adjust the pH between 7.5-8.0, NH 4 Cl 0.5g-1.0g, initial NH 4 -N concentration 150mg / L-300mg / L, NO in the initial medium 3 -N concentration is controlled at 0mg / L-10mg / L.

[0049] Du...

technical field

[0001] The invention belongs to the field of microbial fermentation, and in particular relates to a high-density enrichment method of an autotrophic nitrifying bacteria consortium and the application of the obtained high-density autotrophic nitrifying bacteria consortium to sewage treatment. Background technique

[0002] Autotrophic nitrifying bacteria are collectively referred to as nitrosifying bacteria and nitrifying bacteria consortia. These microorganisms can convert ammonia nitrogen into nitrite nitrogen and then into nitrate nitrogen, and play an important role in the natural N element cycle. Along with the development of microorganism separation technology, this kind of microorganism is applied in various fields, as patent CN101039692A discloses a kind of consortium composed of Nitrosomonaseutropea and Nitrobacter victoria in aquaculture. Subsequently, this type of consortium was widely used in the treatment of ammonia nitrogen wastewater, but the app...

Examples

Embodiment 1

[0030] The reaction device includes a fermenter, a carbon source storage tank, a peristaltic pump A, a pH electrode, a control system, a nitrogen source storage tank, a peristaltic pump B, a dissolved oxygen electrode, a temperature probe, a stirring motor, an air compressor, and a heater. The fermenter is inserted with a pH electrode, a dissolved oxygen electrode and a temperature probe, and the pH electrode, the dissolved oxygen electrode and the temperature probe are respectively connected to the control system through wires, and the fermenter is respectively connected to the external mixer, peristaltic pump A, and peristaltic pump B through wires. , the air compressor and the heater are connected, and the peristaltic pump A, peristaltic pump B, air compressor and heater are respectively connected to the control system through wires. In addition, the peristaltic pump A is connected to the carbon source storage tank and the peristaltic pump through pipelines. B passes through...

Embodiment 2

[0038] When Na 2 CO 3 When the cumulative amount X value of the solution consumption reaches V / 4 (L), the expansion is over, and the nitrification rate of the expansion solution is > 160 mg (NO 3 -N) / L / h. Stop stirring and stand still for 1 hour to collect the autotrophic nitrifying bacteria consortium, and the nitrification rate per unit mass of the harvested consortium is >600 mg (NO 3 -N) / g / h. Example 2 100L-scale automatic expansion of nitrosifying bacteria and nitrifying bacteria copolymers

[0039] The volume of the fermenter is 100L, the consortium of nitrosifying bacteria and nitrifying bacteria is used as the inoculum, the inoculum size is 1%, and the initial volume of the medium is V=80L. Stirring speed is 220r / min, keeps constant in the expansion cultivation process, and the inorganic carbon source is 10% Na 2 CO 3 solution, the inorganic nitrogen source is 20% NH 4 Cl solution. During the expansion process, the dissolved oxygen DO was controlled at 20%, and...

Embodiment 3

[0047] Example 3: Automatic enrichment and expansion of 100L-scale activated sludge autotrophic nitrifying bacteria flora

[0048] The volume of the fermenter is 100L, the activated sludge of the pesticide wastewater biochemical pool is used as the inoculum, the inoculum size is 10%, and the initial volume of the medium is V=80L. Stirring speed is 220r / min, keeps constant in the expansion cultivation process, and the inorganic carbon source is 10% Na 2 CO 3 solution, the inorganic nitrogen source is 20% NH 4 Cl solution. During the expansion process, the dissolved oxygen DO was controlled at 20%, and the pH was controlled at 7.50. Among them, the initial medium of autotrophic nitrifying bacteria per liter of Na in water 2 CO 3 2g-4g, MgSO 4 0.2g-0.4g, KCl 0.2g-0.4g, phosphoric acid to adjust the pH between 7.5-8.0, NH 4 Cl 0.5g-1.0g, initial NH 4 -N concentration 150mg / L-300mg / L, NO in the initial medium 3 -N concentration is controlled at 0mg / L-10mg / L.

[0049] Du...

Images