Preparation method of vaccine for epidemic encephalitis B

Abstract

The invention relates to a preparation method of a vaccine for epidemic encephalitis B, which comprises the processes of cell culture, virus propagation and vaccine preparation. After cells overgrow with a single layer, a seed culture of viruses and a culture solution are inoculated, and the use quantity of the seed culture of the viruses is that every 100cm<2> of cells is inoculated with 1ml of seed culture of the viruses. The culture solution of the viruses mainly comprises 90-95% of MEM culture solution, 3-5% of calf serum and 7.5% of sodium bicarbonate solution the pH of which is regulated to 7.2-7.6. The vaccine is prepared by adding human serum albumin with the final concentration of 0.3% after being clarified and filtered. The invention adopts an SA14-14-2 attenuated strain to produce an encephalitis purified vaccine. Compared with a vaccine produced by a P3 strain, the attenuated strain is safer and more effective. The invention has the advantages that: (1) the attenuated strain is used for preparing the purified vaccine, which has good safety; (2) the inactivation effect of inactivating the viruses by using beta-propiolactone is good, and an inactivator is naturally degraded and has no residue; and (3) the column chromatography purification property is mild, and the injury to the viruses is small.

Description

Technical field

[0001] The invention belongs to a preparation method of a vaccine, and mainly relates to a preparation method of an epidemic Japanese encephalitis vaccine. Background technique

[0002] Japanese encephalitis is collectively referred to as Japanese encephalitis in the world, it used to be called Japanese encephalitis, and it is called Japanese encephalitis (JE) in my country. In my country, chicken embryos and mouse brains were used to make inactivated vaccines, but the immunogenicity of the chicken embryo vaccines was poor, and the mouse brain vaccines contained more mouse brain tissue components, and the side effects and immunity of the vaccines were not satisfactory. Later, hamsters were used. Kidney cells are used to culture virus to prepare vaccine, which not only has less side effects, but also has better effect.

[0003] JE vaccines currently on the market mainly include live vaccines and inactivated vaccines (i.e. purified vaccines). Live vaccines are...

Examples

Embodiment Construction

[0014] Below in conjunction with embodiment the invention will be further described:

[0015] This patent uses SA 14 -14-2 attenuated strain produces purified Japanese encephalitis vaccine, which is an inactivated vaccine. The mother strain virus is the JEV SA14 strain isolated from Xi'an mosquito larvae in 1954. Through the method of multiplying the extraneural tissues of animals to improve immunity, a production strain with low virulence and high immunity (SA 14 -14-2). SA 14 The -14-2 attenuated strain has high thermal stability, and the virus is not completely inactivated when heated at 50°C for 2 to 4 hours, which is no different from the original strain of SA14. Its genotype is also quite stable, and it remains stable in the 17th passage of hamster kidney cell virus.

[0016] The present invention relates to the method for epidemic Japanese encephalitis vaccine, its steps are as follows:

[0017] Choose healthy hamsters aged 10-14 days and weighing 12-15 grams, kill...

Technical field

[0001] The invention belongs to a preparation method of a vaccine, and mainly relates to a preparation method of an epidemic Japanese encephalitis vaccine. Background technique

[0002] Japanese encephalitis is collectively referred to as Japanese encephalitis in the world, it used to be called Japanese encephalitis, and it is called Japanese encephalitis (JE) in my country. In my country, chicken embryos and mouse brains were used to make inactivated vaccines, but the immunogenicity of the chicken embryo vaccines was poor, and the mouse brain vaccines contained more mouse brain tissue components, and the side effects and immunity of the vaccines were not satisfactory. Later, hamsters were used. Kidney cells are used to culture virus to prepare vaccine, which not only has less side effects, but also has better effect.

[0003] JE vaccines currently on the market mainly include live vaccines and inactivated vaccines (i.e. purified vaccines). Live vaccines are...

Examples

Embodiment Construction

[0014] Below in conjunction with embodiment the invention will be further described:

[0015] This patent uses SA 14 -14-2 attenuated strain produces purified Japanese encephalitis vaccine, which is an inactivated vaccine. The mother strain virus is the JEV SA14 strain isolated from Xi'an mosquito larvae in 1954. Through the method of multiplying the extraneural tissues of animals to improve immunity, a production strain with low virulence and high immunity (SA 14 -14-2). SA 14 The -14-2 attenuated strain has high thermal stability, and the virus is not completely inactivated when heated at 50°C for 2 to 4 hours, which is no different from the original strain of SA14. Its genotype is also quite stable, and it remains stable in the 17th passage of hamster kidney cell virus.

[0016] The present invention relates to the method for epidemic Japanese encephalitis vaccine, its steps are as follows:

[0017] Choose healthy hamsters aged 10-14 days and weighing 12-15 grams, kill...

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