Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nanometer antibacterial agent loaded with both nano-silver and curcumin and preparing method and application thereof

A nano antibacterial agent, curcumin technology, applied in the field of nano biomedical materials, can solve the problems of limited clinical development, low bioavailability, poor water solubility of curcumin, etc., achieve good biocompatibility, enhance antibacterial effect, improve stability Effects of Sexuality and Bioavailability

Active Publication Date: 2017-06-23
INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
View PDF1 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to poor water solubility and low bioavailability of curcumin, its clinical development is extremely limited.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nanometer antibacterial agent loaded with both nano-silver and curcumin and preparing method and application thereof
  • Nanometer antibacterial agent loaded with both nano-silver and curcumin and preparing method and application thereof
  • Nanometer antibacterial agent loaded with both nano-silver and curcumin and preparing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] A nano-antibacterial agent loaded with nano-silver and curcumin at the same time, it is an amphiphilic block copolymer PCL- b -PAsp self-assembles in aqueous solution to form PCL-b-PAsp polymer micelles PM, which utilizes the negative charge of PAsp in the hydrophilic shell to adsorb positively charged Ag + ions and reduce them into nano-silver in situ by a reducing agent, and then use the hydrophobic inner core PCL to wrap the natural non-toxic antibacterial component curcumin, thus forming a polymer micelle PM-Ag- which carries both nano-silver and curcumin Cur; wherein the ratio of PCL-b-PAsp polymer micelles PM: nano silver: curcumin is 10:1:2.

[0055] The preparation method of nano antibacterial agent:

[0056] 1) Dissolve 5.0 mg of PCL-b-PAsp in 1 mL of DMF, and slowly add the polymer solution dropwise to 9 mL of phosphate buffer, 1 mM, pH=7.4 under electromagnetic stirring, and then The solution was stirred at room temperature for 4 h to stabilize the micelles...

Embodiment 2

[0069] (1) Polycyclocaprolactone- b -polyaspartic acid (PCL- b -PAsp), the steps are as follows:

[0070] 1) Add 0.16 g N-(tert-butoxycarbonyl)ethanolamine (1 mmol) and 10.0 g ε-CL (ε-caprolactone) (87.8 mmol) into a dry 50 mL eggplant-shaped bottle and mix well, then add 20 mL redistilled toluene, then add a drop of Sn(Oct) 2 (0.1% wt / wt);

[0071] 2) Freeze with liquid nitrogen, vacuumize, ventilate with nitrogen, and thaw, repeat this process three times, and then place the reaction bottle in an oil bath at 110°C for 12 h under the protection of nitrogen;

[0072] 3) After the reaction, cool the reaction bottle to room temperature, add glacial ether to precipitate, let stand to make the precipitation complete, wash by suction filtration, and obtain white solid PCL-NHBoc after vacuum drying;

[0073] 4) Dissolve the PCL-NHBoc obtained in the above 3) in a mixed solvent of trifluoroacetic acid and dichloromethane (1 / 1, V / V), and then stir at a constant speed at room temp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a nanometer antibacterial agent loaded with both nano-silver and curcumin and a preparing method and application thereof. A polymeric micelle formed through self-assembly of the amphiphilic block copolymer PCL-b-PAsp is taken as a nano-carrier, positively charged Ag+ ions are adsorbed by means of the electronegativity of a PAsp chain segment in a hydrophilic shell and converted into nano-silver through in-situ reduction by means of a reductant, the natural and nontoxic antibacterial constituent curcumin is coated with hydrophobic core PCL, and in this way, the polymeric micelle loaded with both nano-silver and curcumin is formed and can be used for improving antibacterial activity. The nanometer antibacterial agent has high synergetic antibacterial efficiency for pseudomonas aeruginosa and staphylococcus aureus. The preparing method is simple, and raw materials have high biocompatibility and biodegradability, are cheap and easy to obtain and can be converted towards the clinical direction easily. Compared with a nanometer antibacterial agent loaded with either nano-silver or curcumin, the nanometer antibacterial agent loaded with both nano-silver and curcumin has the advantage that the synergetic pathogenic bacteria killing effect is good, and the anometer antibacterial agent loaded with both nano-silver and curcumin has broad application prospects.

Description

technical field [0001] The invention belongs to the field of nano biomedical materials, and relates to a preparation method of a nano antibacterial agent loaded with nano silver and curcumin and its application in enhancing antibacterial activity. Background technique [0002] In recent years, the rapid emergence of multidrug-resistant bacteria has become a growing global problem, and the World Health Organization has identified it as one of the three major threats to human health. Therefore, it is urgent to explore new antibacterial agents with good safety and strong antibacterial activity and without bacterial resistance. To this end, various antimicrobial materials, such as antimicrobial peptides, cationic polymers, carbon-based nanomaterials, polymers, and inorganic nanoparticles, have been extensively studied to enhance antibacterial properties. However, monotherapies are no longer sufficient to completely tackle complicated bacterial infections. For example, doctors ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K9/107A61K47/34A61K33/38A61P31/04A61K31/12
CPCA61K9/1075A61K31/12A61K33/38A61K47/34A61K2300/00
Inventor 刘鉴峰黄帆刘金剑张玉民高阳褚丽萍杨丽军任春华
Owner INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com