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A kind of affinity biomembrane, preparation method and application thereof

A biofilm and affinity technology, applied in the field of affinity biofilm and preparation, can solve the problems of low adsorption efficiency, difficult to popularize and use in a wide range, high production cost, and achieve improved antifouling performance, improved hydrophilic performance and high production cost. Mechanical properties, the effect of improving the service life

Inactive Publication Date: 2018-01-26
HENAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Some commercialized products also have problems such as low adsorption efficiency and high production costs, making it difficult to promote and use them on a large scale

Method used

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  • A kind of affinity biomembrane, preparation method and application thereof
  • A kind of affinity biomembrane, preparation method and application thereof
  • A kind of affinity biomembrane, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation of Affinity Biofilm Using Polyethyleneimine or Polymyxin B as Ligand:

[0028] (1) Preparation of amphiphilic carbon materials (ACPs): take 10 g of brewer's yeast (provided by Henan Oak Brewery) and shake to disperse evenly, take the gray-white bacterial suspension in the upper layer and centrifuge, discard the waste liquid, and then add 10~ 15ml of acetone, place on a shaker and shake at 180 r / min for 10-20 min, centrifuge, discard the acetone supernatant, repeat the same method 3-5 times to obtain the cell wall of brewer's yeast, and use the substrate (the obtained cell wall) with Disperse 100ml of 2% (v / v) glutaraldehyde PBS buffer solution with pH=6.0, pour it into a high-temperature and high-pressure reaction kettle, put it into an oven, and hydrothermally carbonize it at 150-200°C for 8-10 hours. After the reaction, cool To room temperature, pour off the upper layer solution, add 100~150ml of distilled water to the lower layer of sediment for ultrasoni...

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Abstract

The invention discloses a biological affinity membrane, a preparation method and application thereof. The biological affinity membrane is made by using an amphipathic carbon material as matrix carrier, grafting affinity ligands on the surface of the amphipathic carbon material by adopting a covalent binding method and using the functionalized amphipathic carbon material as an additive to be blended with a hydrophobic membrane material. The functionalized amphipathic carbon material is obtained by evenly fixing the affinity ligands to the surface of the amphipathic carbon material by means of the covalent binding method and is used as the additive to prepare the affinity membrane. The prepared biological affinity membrane is of a micropore / macroporous structure, has large inner surface area, high connectivity and mechanical stability and can remove endotoxin in a protein solution in a high-selectivity mode.

Description

technical field [0001] The invention belongs to the field of membrane technology, and in particular relates to an affinity biological membrane, a preparation method and an application thereof. Background technique [0002] Endotoxin is a highly toxic inflammatory and pyrogenic substance, and is the main pathogenic medium of endotoxemia and septic shock. Low doses of endotoxin can induce systemic inflammatory response syndrome (SIRs), multiple organ Dysfunction Syndrome (MODS), sepsis, etc. are leading to significant mortality in all age groups. [0003] With the development of related fields of biopharmaceutical and biomedical engineering, people pay more and more attention to the removal of trace harmful substances that may exist in biological products. Due to the relatively large molecular weight of endotoxin, which is on the same order of magnitude as that of macromolecular biological products, effective removal of endotoxin from products, especially biological products,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J5/18C08L27/16C08L39/06C08K9/04C08K3/04
CPCC08J5/18C08J2327/16C08J2439/06C08L27/16C08L2203/16C08L39/06C08K9/04C08K3/04
Inventor 吴强杨凯李旭鹏徐玥玥崔海磊张旭
Owner HENAN UNIVERSITY
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