Water-soluble medicinal fertilizer containing strong-antagonism anti-rice blast bacterial strain, preparation method and application of medicinal fertilizer

A kind of rice blast fungus, water-soluble technology, applied in the field of water-soluble medicinal fertilizer and its preparation, to achieve the effects of advanced scientific preparation method, enhanced invasion ability, and promotion of healthy growth

Inactive Publication Date: 2015-02-18
HUNAN RANSUN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many scholars at home and abroad have isolated and screened antagonistic strains to control rice blast from soil and rice tissues, but few have isolated from weeds that are rich in resources and have a similar growth environment to crops.

Method used

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  • Water-soluble medicinal fertilizer containing strong-antagonism anti-rice blast bacterial strain, preparation method and application of medicinal fertilizer
  • Water-soluble medicinal fertilizer containing strong-antagonism anti-rice blast bacterial strain, preparation method and application of medicinal fertilizer
  • Water-soluble medicinal fertilizer containing strong-antagonism anti-rice blast bacterial strain, preparation method and application of medicinal fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 contains the water-soluble medicinal fertilizer of strong antagonistic rice blast strain

[0043] Preparation of Bacillus subtilis-W3 spore powder in HR biocontrol agent:

[0044] 1. Slant culture: inoculate the Bacillus subtilis-W3 strain on the slant medium under aseptic conditions: glucose 15g, fish peptone 5g, yeast extract 5g, water 1000mL, agar 15g, cultivate at 30°C for 48h;

[0045] 2. Shaker culture: inoculate the bacterial classification cultivated in step 1 in the liquid medium under aseptic conditions: corn flour 13g, glucose 5g, bean cake powder 20g, fish meal 5g, CaCO 3 6g, (NH 4 ) 2 SO 4 1g, K 2 HPO 4 0.3g, MgSO 4 7H 2 O 0.2g, MnSO 4 ·H 2 O 0.2g, water 1000ml, under the conditions of pH 6.5 and temperature 30°C, cultivate on a shaker at 160r / min for 36h;

[0046] 3. Fermentation tank culture: inoculate the strains cultivated in step 2 in the fermentation tank medium under aseptic conditions: 26 kg of corn flour, 16 kg of bean ca...

Embodiment 2

[0050] Embodiment 2 contains the water-soluble medicinal fertilizer of strong antagonistic rice blast strain

[0051] Compared with Example 1, the difference is that the composition of the water-soluble medicinal fertilizer containing strong antagonistic rice blast strains in this example is: 55 parts of HR biocontrol bacteria agent, 30 parts of γ-PGA, and 15 parts of plant cell solidin.

[0052] Wherein, the preparation of Bacillus subtilis-W3 spore powder in the HR biocontrol agent:

[0053] 1. Slant culture: Inoculate the original strain of Bacillus subtilis on the slant medium under sterile conditions, and culture at 29°C for 48 hours;

[0054] 2. Cultivation on a shaker: Inoculate the bacteria cultured in the above step 1 in a liquid medium under sterile conditions, and culture on a shaker at 160r / min for 36 hours at pH 6.5 and a temperature of 29°C;

[0055] 3. Fermentation tank culture: Inoculate the strains cultivated in the above step 2 into the fermenter medium unde...

Embodiment 3

[0060] Embodiment 3 contains the water-soluble medicinal fertilizer of strong antagonistic rice blast strain

[0061] Compared with Example 1, the difference is that the composition of the water-soluble medicinal fertilizer containing strong antagonistic rice blast strains in this example is: 85 parts of HR biocontrol bacterial agent, 10 parts of γ-PGA, and 5 parts of plant cell solidin.

[0062] Wherein, the Bacillus subtilis-W3 in the HR biocontrol bacterial agent is prepared by the following steps:

[0063] 1. Slant culture: inoculate the original strain of Bacillus subtilis on the slant medium under sterile conditions, and culture at 30°C for 48 hours;

[0064] 2. Cultivation on a shaker: inoculate the bacteria cultured in the above step 1 into the liquid medium under sterile conditions, and culture on a shaker at 160 r / min for 48 hours at pH 6.5 and a temperature of 29°C;

[0065] 3. Fermentation tank culture: Inoculate the strains cultivated in the above step ② into the...

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Abstract

The invention provides a water-soluble medicinal fertilizer containing a strong-antagonism anti-rice blast bacterial strain, a preparation method and application of the medicinal fertilizer. The medicinal fertilizer is prepared from the following constituents in parts by weight: 55-85 parts of an HR (Hypersensitive Response) antibiological inoculant, 10-30 parts of polyglutamic acid, and 5-15 parts of a plant cell stabilizer, wherein the HR antibiological inoculant comprises bacillus subtilis-w3 with an effective viable count of 7.0-9.0*10<9> cfu/g. The medicinal fertilizer disclosed by the invention can achieve a good biological control effect on rice blast, banded sclerotial blight and the like, significantly promotes growth of the rice, increases number of productive ears, setting percentage and thousand seed weight of the rice, and remarkably improves the vegetable yield and the quality.

Description

technical field [0001] The invention relates to the field of microbial medicinal fertilizers, in particular to a water-soluble medicinal fertilizer containing strong antagonistic rice blast strains and a preparation method and application thereof. Background technique [0002] Rice blast (Pyricularia grisea) is an important disease widely distributed in various rice producing areas in the world. It occurs in the vast rice areas in the north and south of my country. For a long time, most of the rice areas in my country have mainly used fungicides such as isoprothiolane and tricyclazole for control. Long-term use of fungicides in large areas can easily lead to the occurrence of rice blast bacteria Drug resistance, and cause environmental pollution and damage to ecological balance. Therefore, the biological control of plant diseases is considered to be an important control method with development potential. Many scholars at home and abroad have isolated and screened antagonist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/04C05G3/80
CPCC05D9/00C05G3/00C05G3/80
Inventor 胡九洲孟三宏刘海涛唐毓杰
Owner HUNAN RANSUN BIOTECH
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