Microbial source bactericide for preventing and treating plant diseases and preparation method thereof

A microbial source and plant disease technology, applied in the field of microbial pesticides, can solve the problems of soil compaction and salinization, the decline of agricultural product yield, and the great influence of farmers, and achieve the effect of promoting secretion, increasing secretion, and improving the control effect

Active Publication Date: 2020-12-01
JIANGXI NEW DRAGON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the long-term continuous cropping of crops, soil pathogens have accumulated for a long time, and soil-borne diseases have occurred seriously. In addition, the improper use of chemical pesticides and fertilizers has led to serious soil compaction and salinization. Low, great impact on farmers

Method used

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  • Microbial source bactericide for preventing and treating plant diseases and preparation method thereof
  • Microbial source bactericide for preventing and treating plant diseases and preparation method thereof
  • Microbial source bactericide for preventing and treating plant diseases and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] PDA medium:

[0036] Fresh potato 200g, agar 16g, glucose 20g, distilled water 1000mL; method: peel the potato and cut into small pieces of about 1cm3, put it in a pot filled with 800mL distilled water and boil for 30min, filter it with gauze, after filtering, take the filtrate and add glucose, After the agar was boiled, it was subpackaged and autoclaved at 121°C for 20 minutes for later use.

[0037] Solid state fermentation medium:

[0038] Wheat bran 60g, rice husk 15g, corn flour 5g, soybean meal 15g, water content 60%, pH adjusted to 7.0; autoclave at 121°C for 20 minutes before use.

[0039] A microbial source fungicide for preventing and treating plant diseases, the raw material components include: in parts by weight, 30 parts of fermentation broth, 60 parts of carriers, and 15 parts of auxiliary agents; wherein, the biocontrol bacteria include: in parts by weight, 40 parts Parts of Trichoderma harzianum, 30 parts of Paenibacillus polymyxa, 15 parts of Bacillus...

Embodiment 2

[0046] The preparation of PDA medium and solid-state fermentation medium is the same as in Example 1.

[0047] A microbial source fungicide for preventing and treating plant diseases, the raw material components include: in parts by weight, 25 parts of fermentation broth, 60 parts of carriers, and 15 parts of auxiliary agents; wherein, the biocontrol bacteria include: in parts by weight, 40 parts Parts of Trichoderma harzianum, 30 parts of Paenibacillus polymyxa, 15 parts of Bacillus licheniformis, 15 parts of Bacillus subtilis; the carrier includes vermiculite and MIL series materials, and the mass ratio is 1.2:1; the auxiliary agent includes: dispersant MF -6. The wetting agent 9500 and the protecting agent honey, the mass ratio is 3.2:4.8:1; the addition amount of kainic acid in the solid medium is 0.5%.

[0048] The steps of the preparation method are the same as in Example 1.

Embodiment 3

[0050] The preparation of PDA medium and solid-state fermentation medium is the same as in Example 1.

[0051] A microbial source fungicide for preventing and treating plant diseases, the raw material components include: in parts by weight, 25 parts of fermentation broth, 60 parts of carriers, and 15 parts of auxiliary agents; wherein, the biocontrol bacteria include: in parts by weight, 40 parts Parts of Trichoderma harzianum, 30 parts of Paenibacillus polymyxa, 15 parts of Bacillus licheniformis, 15 parts of Bacillus subtilis; the carrier includes vermiculite and MIL series materials, and the mass ratio is 1.2:1; the auxiliary agent includes: dispersant MF -6. The wetting agent 9500 and the protecting agent honey, the mass ratio is 3.2:4.8:1; the addition amount of kainic acid in the solid medium is 1.5%.

[0052] The steps of the preparation method are the same as in Example 1.

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Abstract

The invention provides a microbial source bactericide for preventing and treating plant diseases and a preparation method thereof, and belongs to the technical field of microbial pesticides. The preparation method of the microbial source bactericide comprises the following steps: performing slant culture on a biocontrol strain to obtain an activated strain; inoculating into a liquid culture mediumand culturing to obtain liquid seeds of each strain; transferring the liquid seeds of each strain into a solid-state fermentation culture medium containing red alga amino acid, carrying out fermentation culture, soaking in sterile water, and filtering to obtain fermentation liquor of each strain; and mixing the fermentation liquor, compounding with a carrier and an auxiliary agent, carrying out spray drying, and carrying out jet milling to obtain the microbial source bactericide. The microbial source bactericide prepared by the method has the advantages of good suspension rate, short wettingtime, strong bacteriostasis and obvious control effect on plant diseases.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a microbial source fungicide for preventing and controlling plant diseases and a preparation method thereof. Background technique [0002] The loss caused by crop diseases accounts for about 25% to 75% of the total output. Taking rice, the main food crop in my country, as an example, the annual planting area is about 400 million mu, and the yield per mu is 400 kg. Cause economic losses to reach more than 300 billion yuan huge, seriously threaten the safe production of food crops. At present, the control of plant diseases in production mainly depends on spraying chemical fungicides in addition to selecting improved varieties and improving cultivation measures. Most of the chemical fungicides currently used have varying degrees of toxicity to humans and animals, and the harmful components remaining in the edible parts of plants will pose a potential threat ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/22A01N63/25A01N63/38A01N25/14A01N25/08A01P21/00A01P3/00C12N1/20C12N1/14C12R1/125C12R1/10C12R1/01C12R1/885
CPCA01N25/08A01N25/14A01N63/22A01N63/25A01N63/38C12N1/14C12N1/20
Inventor 不公告发明人
Owner JIANGXI NEW DRAGON BIOTECH
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