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Bacillus subtilis and application thereof

A technology of Bacillus subtilis and strain, applied to Bacillus subtilis and its application field, can solve the problems of low crude protein and small peptide content, long fermentation time, incomplete degradation of anti-nutritional factors, etc., so as to improve nutritional quality and promote absorption effect

Active Publication Date: 2013-12-25
浙江科峰生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, it is reported that there are many problems in the microbial strains produced, such as long fermentation time (so it is easy to cause mildew), excessive moisture in the fermentation process, incomplete degradation of anti-nutritional factors, crude protein and small peptides (acid-soluble protein) after fermentation The problem of low content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Isolation, purification, mutagenesis and identification of Bacillus subtilis strain KF-2

[0020] Bacillus subtilis was screened under natural conditions, and after multiple rounds of mutation breeding by our company, the strain has been genetically improved, its ability to degrade and transform soybean meal has been significantly improved, and the production performance of the strain has been greatly improved.

[0021] The beef extract peptone medium plate was prepared, and Bacillus subtilis was isolated and cultured from organic matter such as beans, and the obtained strain was identified as Bacillus subtilis by colony observation and morphological microscopic identification.

[0022] Use Bacillus subtilis to ferment soybean meal, repeatedly screen and purify and rejuvenate (steps: isolate and cultivate Bacillus subtilis from better fermented soybean meal products, obtain purified strains and continue to cultivate, and then further ferment soybean meal to co...

Embodiment 2

[0034] Embodiment 2: Utilize Bacillus subtilis KF-2 fermentation to produce the method that is rich in active peptide protein feed

[0035] 1) The Bacillus subtilis KF-2 is activated on plate medium, seed shake flask culture, primary shake flask culture or fermentation tank is gradually enlarged to obtain seed liquid, the above plate medium, shake flask medium Both are PDA medium; the plate medium is activated by transferring the strains stored on the slant to the plate, culturing at 32°C, and then picking 3 single colonies from the plate to the seed shake flask, and the seed shake flask is heated at 32°C and 150 r / min Under shaking table conditions, after culturing for 15 h, all the seed culture liquid was inoculated into the fermenter, and the fermenter was ventilated for 24 h.

[0036] The above plate medium is: beef extract, peptone medium (5 g beef extract, 10 g peptone, 5 g sodium chloride, 15-20 g agar, 1000 mL distilled water, pH 7.0-7.2), seed shake flask medium For:...

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PUM

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Abstract

The invention relates to bacillus subtilis and an application thereof, and belongs to the technical field of biological engineering. The invention provides a bacillus subtilis strain KF-2 with a preservation number of CGMCC No. 7690. Besides, the invention provides a method for applying the bacillus subtilis in fermentation production of active peptide-rich protein feed. A plurality of indexes are determined after soybean meal is fermented by the strain. Crude protein content reaches over 51%; acid soluble protein content reaches over 6%; viable counts reach 2 billion per gram of the fermented soybean meal; and volatile salt-based nitrogen is lower than 50 mg / 100 g.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a bacillus subtilis and an application thereof. Background technique [0002] Feed-grade microbial additives are a new type of feed additives developed in recent years. They have a significant role in improving feed utilization, increasing production performance, regulating intestinal micro-ecological balance, and increasing immunity. At present, the most commonly used microorganisms in feed and animal production include lactic acid bacteria, yeast, bacillus and photosynthetic bacteria. Among them, bacillus has unique biological characteristics such as strong stress resistance, high temperature and high pressure resistance, acid and alkali bile salt resistance, and easy storage. , from the perspective of animal production and feed industry, it can better meet the requirements of modern industrial production of microorganisms for feed. [0003] Bacillus subtili...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/14C12R1/125
Inventor 章亭洲
Owner 浙江科峰生物技术有限公司
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