Method for producing citrin and saponin from tuber crops by biotechnology

A biotechnology, citric acid technology, applied in the direction of biochemical equipment and methods, fermentation, etc., can solve the problems of low profit margin, serious pollution profit margin, high energy consumption, etc., and achieve the effect of improving quality and output

Inactive Publication Date: 2005-09-28
刘华祥 +3
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Problems solved by technology

The discharge of industrial wastewater and waste residues pollutes the environment, and the investment in this process is large. Take 10,000 tons of citric acid as an example
It requires an investm

Method used

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Embodiment Construction

[0017] The present invention utilizes biotechnology to produce the method for citric acid by yam plant, and concrete steps are as follows:

[0018] A Screening strains: re-screen the optimal citric acid-producing strains suitable for tuber plants (turmeric) with the original citric acid-producing Aspergillus niger strains to obtain Aspergillus niger strains.

[0019] B. The yam plants are planned to be purchased and stored in cold storage. When used, take 100Kg out, wash the tubers, add 50% water to pulverize, beat, and use the cultivated Bacillus subtilis, lactic acid bacteria or directly produce amylase, cellulase and organic matter degradation enzymes. The fungus liquid is added to 50% of the weight of yam and taro roots, stirred evenly, soaked, adjusted to PH = 7.3, enzymatically hydrolyzed at a temperature of 45-55°C for 20 minutes, and heated to 80-90°C for 1 hour of heat preservation and liquefaction. Then add water according to 15-20% of root tuber weight, sterilize, c...

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Abstract

The biological technological process of producing citric acid and saponin with yam plant material includes the biological technological process of producing citric acid with yam plant material and the biological technological process of producing saponin with the yam plant material residue. The present invention has the advantages of raised citric acid production utility and reduced environmental pollution caused by saponin production. The present invention has greatly raised saponin yield and quality, citric acid converting rate as high as 35 % and yield of 85 % and saponin yield of 0.6-0.8 %.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for producing citric acid and saponin from yam plants using biotechnology. Background technique [0002] For many years, citric acid and saponin have been produced from cassava, dried sweet potato, corn, wheat, rice, beet sugar, glucose mother liquor, etc., and the production of citric acid and saponin is carried out separately. All waste is wasted. Generally, cassava is used as the main raw material for production. The process is to crush cassava, ferment Aspergillus niger, then extract, refine, crystallize, centrifuge, and dry to obtain the finished product. The discharge of industrial waste water and waste residue pollutes the environment, and the investment in this process is large. Take 10,000 tons of citric acid as an example. It requires an investment of 30 million yuan, which consumes a lot of energy and has low profit margins. It is a product...

Claims

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Application Information

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IPC IPC(8): C12PC12P7/48C12P33/00
Inventor 刘华祥曾庆祥代自强李长荣
Owner 刘华祥
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