Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Streptomyces virginiae and its application in transformation and degradation of steroid

A technology of Streptomyces virginis and steroid compounds, which is applied to Streptomyces virginis and its application fields, and can solve the problems of low yield, high price, and many steps.

Inactive Publication Date: 2006-12-13
EAST CHINA UNIV OF SCI & TECH
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This has fundamentally changed the shortcomings of the diosmin method in the production of certain steroid drugs, such as many steps, low yield, and high price.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Streptomyces virginiae and its application in transformation and degradation of steroid
  • Streptomyces virginiae and its application in transformation and degradation of steroid
  • Streptomyces virginiae and its application in transformation and degradation of steroid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Transformation and degradation of diosgenin by Streptomyces virginia IBL-14

[0052] In 30 ml of medium in a 250 ml Erlenmeyer flask, under the condition of 30°C, strain IBL-14 was cultured for 18 hours at the first level, and then 3 ml of the culture was inoculated into 30 ml of the second level medium. When the culture is in the logarithmic phase, that is, 3 hours to 9 hours, 1 milliliter of diosgenin ethanol solution with a concentration of 0.02 g / ml is added to 30 milliliters of culture fluid, and the culture is continued, and the progress of the reaction is monitored by TLC and HPLC analysis methods In this case, there is no obvious accumulation of metabolic intermediates, and the degradation results are the same as figure 1 shown.

[0053] The components and concentrations of the primary seed medium and the secondary medium all used the fermentation medium shown in Table 1.

Embodiment 2

[0055] Transformation and degradation of diosgenin by Streptomyces virginia IBL-14

[0056] In 30 ml of medium in a 250 ml Erlenmeyer flask, under the condition of 30°C, strain IBL-14 was cultured for 18 hours at the first level, and then 4 ml of the culture was inoculated into 30 ml of the second level medium. At the end of the logarithmic growth phase (about 15 hours), add 1 milliliter of diosgenin ethanol solution with a concentration of 0.02 g / ml into 30 milliliters of culture fluid, continue to cultivate, and monitor the progress of the reaction with TLC and HPLC analysis methods , the result is the same as figure 2 as shown, figure 2 Among them, 1 represents diosgenin, 2 represents diosgenone, and 3 represents 25-hydroxydioscinone. There are obvious accumulation of metabolic intermediates, and two intermediates can be accumulated in large quantities, namely 3-keto-4-ene-spiro Steroid (spirost-4-en-3-one, diosgenone) and 3-keto-4-ene-25-hydroxy spiroster (25-hydroxysp...

Embodiment 3

[0058] Transformation and degradation of diosgenin by Streptomyces virginia IBL-14

[0059] In 30 ml of medium in a 250 ml Erlenmeyer flask, at 30°C, strain IBL-14 was cultured to the end of logarithmic growth in the primary stage, and then 3 ml of the culture was inoculated into 30 ml of the secondary medium In the second stage of the logarithmic growth phase (about 15 hours), 1 milliliter of diosgenin ethanol solution with a concentration of 0.02 g / ml was added to 30 milliliters of culture fluid, and the culture was continued, and the reaction was monitored by TLC and HPLC analysis methods In this case, the metabolites of diosgenin were monitored by LC-MS to obtain the products 1-dehydrodiosgenone and 1-dehydroisonuatigenone.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a Streptomyces virginiae and discloses Streptomyces virginiae IBL-14 CCTCC M 206045 and its application in converting and degrading the steroid compound. The bacterial of this invention can effectively convert and degrade almost all kinds of steroid compounds. This bacterial is an actinomycete which can widely exist in physical environment so that it can be used in environmental conservation domain to clear the steroid contaminant. The said bacterial of this invention can convert and degrade the steroid compound to produce some new compounds, which has potential pharmaceutical activity, for example, the said bacterial can convert yam saponin to produce 25-hydroxy dioscoreassapoketone.

Description

technical field [0001] The present invention relates to Streptomyces virginiae and its application. Background technique [0002] Steroid drugs play a very important regulatory function on the body. The transformation and degradation of steroidal compounds by microorganisms has always been an important research content in the field of steroidal pharmaceutical industry and environmental protection: [0003] 1. Microbial transformation of steroids [0004] The formal application of microbial transformation in the field of pharmaceutical industry began with the development of steroidal drugs. In 1952, Peterson and Murry creatively used Rhizopus niger to carry out α-hydroxylation at the 11th position of progesterone, which made cortisone available. And the yield is as high as 90%. This creative work has aroused great interest of scientists from all over the world, so that microbial transformation can develop rapidly in the field of organic chemical industry, thus forming an in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12P1/04C12R1/465
Inventor 魏东芝童望宇王风清
Owner EAST CHINA UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com