53 results about "Streptomyces thermoautotrophicus" patented technology

The invention discloses application of streptomyces griseoflavus in biological prevention and control of plant diseases, particularly streptomyces griseoflavus NMG6-3-9 CGMCC No. 3441 in biological prevention and control of plant diseases. The provided strains have good antagonism effect on pathogenic bacteria of alfalfa root rot, can be used for the biological prevention and control of alfalfa root rot and other plant diseases, and have wide application potential in the field of biological prevention and control of plant diseases. The invention lays a foundation for the biological prevention and control of alfalfa root rot and further provides a scientific basis for the development and the application of a biological prevention actinomycete preparation.

The invention relates to gopalamicin derivatives and application of the same in inhibition of infection by drug-resistant bacteria and drug-resistant mycobacterium tuberculosis. The derivatives are originated from fermentation products of marine actinomycete Streptomyces sp. 7-145. Results of experimental study show that discovered compounds with novel structures and known structures all have strong antibacterial activity on tested drug-resistant bacteria and drug-resistant mycobacterium tuberculosis and are expected to become clinically-useful novel drugs used for inhibition of infection by drug-resistant bacteria and drug-resistant mycobacterium tuberculosis.

The invention discloses engineering bacillus subtilis capable of expressing phospholipase D, and belongs to the technical field of gene engineering. The engineering bacillus subtilis capable of expressing the phospholipase D is constructed by taking bacillus subtilis (WB600) as an expression host and taking a phospholipase D encoding gene (PLD) from streptomyces racemochromogenes as a target gene.The activity of the phospholipase D produced by the engineering bacteria can be up to 5.916 U/mL and has an extremely high application value in the field of medicines, food and health care products;meanwhile, the phospholipase D adopted by the engineering bacteria is actinomyces-derived endogenous phospholipase D, which has high reaction activity, high phosphatidyl substrate selectivity and highorganic matter stability; furthermore, a catalysis structure is the most compact; the phospholipase D is more suitable for efficient synthesis of phospholipid and a phospholipid derivative in the industrial production.

The invention relates to a novel actinomycin A, a preparation and synthesis method thereof and an application of a compound to anti-drug-resistant bacteria and tumor resistance. The novel actinomycin A is a fermentation product of a marine streptomyces IMB094 (Streptomyces sp. IMB094). Experimental research results show that the novel actinomycin A has high antimicrobial activities for tested Gram-positive resistant bacteria and high inhibiting effects on tested tumor cells, and novel actinomycin A is expected to be novel clinical useful anti-drug-resistant bacteria and tumor-resistant medicines.

The invention discloses a method capable of promoting growth of rice in a normal growth environment and capable of promoting the rice to absorb an iron element in an environment lacking of directly available iron. According to the method, a strain of Streptomycessp. OsiSh-2 is utilized, and a spore suspension thereof is employed; the rice under the normal condition can be treated with two different measures: (1) the spore suspension is sprayed on leaf surfaces of the rice, and (2) disinfected rice seeds are embedded with the spore suspension of Streptomycessp. OsiSh-2. The method disclosed bythe invention promotes the growth of the rice, and increases the chlorophyll content and the photosynthesis rate, and thus, rice biomass including plant height, root length, the fresh and dry weight of aboveground and underground parts and the like of the rice is significantly increased; and under the adverse situation of iron deficiency, the disinfected rice seeds are embedded with the spore suspension of Streptomycessp. OsiSh-2, so that the iron content in the rice is significantly increased, the normal growth of the rice under iron deficiency stress is guaranteed.

The invention provides a compound streptodepsipeptide P11B having anti-glioma activity, wherein the streptodepsipeptide P11B is a marine natural active substance, and is obtained by carrying out separation culture purification in Streptomyces parvulus P11-23B. According to the present invention, the test results prove that the streptodepsipeptide P11B can significantly inhibit the proliferation of a variety of glioma cells, and can be used in preparation of drugs for glioma treatment, wherein the drugs comprise the separate streptodepsipeptide P11B active component and a pharmaceutically acceptable carrier, or the streptodepsipeptide P11B active component, other drugs or effective components, and a pharmaceutically acceptable carrier; the anti-glioma activity of the compound is firstly discovered, and the new way is provided for the preparation of the anti-glioma drug; and the chemical structure formula of the streptodepsipeptide P11B is defined in the specification.

The invention belongs to the technical field of metabolite extraction, and discloses a method for separating and extracting secondary metabolites of streptomyces, which comprises the following steps:carrying out elution on a fermentation broth by using an HP-20 resin column, performing chromatographic separation by a silica gel column and a Sephadex LH-20 gel column, and performing analysis and purification of a compound by (semi) preparing a high performance liquid chromatography. Modern instrument spectrum analysis technologies such as nuclear magnetic resonance and high-resolution mass spectrometry are used for identifying the structure of the monomeric compound. According to the invention, actinomycetes which are separated from a soil sample and have antibacterial and anti-tumor cellactivity are fermented, and induction research is carried out on bioactivity of metabolites and part of products generated by the actinomycetes, so that diversity of microbial compounds is enriched; areference and theoretical basis is provided for research and exploration of active substances produced by other actinomycetes; meanwhile, a premise is provided for exploring and developing active natural compounds and commercial drugs.

The invention provides a roof greening substrate, and belongs to the field of food waste recycling. Particularly, food waste is used as a raw material, dry-wet separation is carried out, fermenting iscarried out by using thermoactinomycetes, ageing is carried out, inoculating of nitrogen-fixing bacteria and phosphate-solubilizing and potassium-solubilizing bacteria is carried out, and finally mixing with conventional materials such as peat is carried out, so that the roof greening substrate is obtained. The substrate provided by the invention is viable, is rich in nutrition and has a certaincapability of inhibiting infectious microbes, the nitrogen-fixing bacteria and the phosphate-solubilizing and potassium-solubilizing bacteria can rapidly grow and reproduce, nutrients are provided forplants, and the growth speed of the plants is high.

The invention provides composite agricultural microorganism bacteria and a method for applying composite bacteria to lotus root planting. All strains of compound bacteria comprise, by weight, 20% of rhodospirillum, 10% of nitrospira, 10% of azotobacter, 10% of bacilli, 10% of thermoactinomyces, 10% of xanthomonas, 10% of lysobacter, 10% of acidophilic bacteria and 10% of novosphingobium. The composite bacteria are used for lotus root planting, the yield of lotus roots is increased, and the quality of the lotus roots is improved.

The invention discloses lipase L-1 and a coding gene and application thereof. The lipase gene l-1 is obtained from Streptomyces sp. SCSIO 13580 in a cloned mode, the nucleotide sequence of the lipase gene l-1 is shown as SEQ ID NO.1, the overall length of the lipase gene l-1 is 1005 bp, and the amino acid sequence of the coded lipase L-1 is shown as SEQ ID NO.2 and contains 334 amino acids in all. The lipase gene l-1 is cloned and connected with an expression vector pET-28a(+), then conversion is performed to obtain escherichia coli BL21 (DE3), and after culture and induced expression, the recombinant expression lipase L-1 is obtained. The lipase L-1 serving as a catalyst catalyzes a reaction between cinnamyl alcohol and an acyl donor, and cinnamyl acetate is prepared, wherein the yield of the obtained cinnamyl acetate can reach 48.3%. The lipase L-1 has the advantages of being high in stability and catalysis efficiency and can be applied to the fields of biological medicine, cosmetics, fine chemical engineering and the like.

The invention relates to a preparation method for a novel antifungal compound and an antifungal application. The invention discloses a novel compound with a structure formula I as follows, wherein the novel compound is prepared by separating Streptomyces-albolongus which is used as a source; the chemical name is (1beta, 4beta, 4abeta, 8a alpha)-4,8a-dimethyloctahydronaphthalene-1,4a(2H)-diol; the molecular formula is C12H22O2. The invention also provides a preparation method and an application of the compound. The method provided by the invention comprises the following steps: performing fermenting culture on the strain S.albolongus, and then centrifuging, thereby acquiring a culture solution; absorbing the culture solution by a macroreticular resin column, reducing pressure and concentrating the 95% alcohol eluting part, thereby acquiring a crude extract; treating the crude extract with normal phase silica gel, dextrangel LH-20 and reverse phase silica gel column chromatography, thereby acquiring a monomer compound shown as structure I; and adopting nuclear magnetic resonance spectrum and mass spectrum for identifying the structure as the novel compound. An in vitro antifungal activity research proves that the compound I provided by the invention has obvious inhibiting effect to various pathogenic fungi, including candida albicans, candida parapsilosis and cryptococcus neoformans and is expected to be developed into a novel antifungal drug.

The invention provides thermophilic streptomyces hygroscopicus cDNA library construction, and expression and expression methods of two greenhouse farmer's lung pathopoiesia antigen polypeptides. The problem needed solving is that: the thermophilic streptomyces hygroscopicus cDNA library is constructed, two greenhouse farmer's lung pathopoiesia virulence genes with virulence effects need screening out for cloning in vitro and inducing expression of the two greenhouse farmer's lung pathopoiesia antigen polypeptides. The key point of construction of the thermophilic streptomyces hygroscopicus cDNA library comprises: acquiring 978 sense sequences and 347 single genes, wherein the homology of two gene fragments respectively with actinobacillus pleuropneumoniae outer membrane protein and transferrin B is 51% and 42%, and the open reading frame length of the two gene fragments respectively is 1554 bp and 726 bp, and 517 and 241 amino acids are respectively encoded. The applications of the thermophilic streptomyces hygroscopicus cDNA library comprise that: from the thermophilic streptomyces hygroscopicus cDNA library, the two greenhouse farmer's lung pathopoiesia virulence genes with virulence effects are screened out for cloning in vitro and inducing expression of the two greenhouse farmer's lung pathopoiesia antigen polypeptides, and theoretical basis and practical basis are provided for subsequent development of new diagnosis methods for farmer's lung diseases.