Application of microorganism bacterium agent in resisting of potato scab
A technology of potato scab, microbial agent, applied in the field of agriculture and biology
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Embodiment 1
[0019] Embodiment 1: the preparation of fermented liquid
[0020] 1) Inoculation: prepare solid culture medium of various strains, carry out strict aseptic operation after sterilization, transfer from inclined plane to flat plate, and cultivate at optimum temperature for 2 to 3 days;
[0021] 2) Primary culture: Pick a single colony from the well-grown plate and transfer it to a 50mL Erlenmeyer flask filled with primary liquid medium, perform aseptic operation, and culture at 30°C and 130r / min for 24-48 hours;
[0022] 3) Secondary fermentation: Inoculate the primary culture strains into a 500mL Erlenmeyer flask filled with secondary liquid medium, perform aseptic operation, and culture at 30°C and 130r / min for 24-48h to obtain Bacillus subtilis ( Bacillus subtilis) CGMCC1.3382 fermentation broth, Dactylosporangium sp. ACCC No.40661 fermentation broth, Streptomyces roseoflavus ACCC No.40400 fermentation broth.
[0023] Medium used in the above method:
[0024] The solid cult...
Embodiment 2
[0027] Embodiment 2: Optimization of fermentation strain culture conditions
[0028] For the Bacillus subtilis (Bacillus subtilis) CGMCC1.3382 fermentation liquid obtained by culturing in Example 1, the actinomycete (Dactylosporangium sp.) ACCC No.40661 fermentation liquid, and the Streptomyces roseoflavus (Streptomyces roseoflavus) ACCC No.40400 fermentation liquid were respectively Mix according to the mass percentage of 30-35%, 30-35%, 33-38%, and inoculate the mixed bacterial solution in the fermenter containing the fermentation medium, the inoculation amount is 1-5% of the total fermentation medium mass. %, cultured at 30°C for 4 to 7 days, so that the number of bacteria in the fermentation broth reached 10×10 8 individual / mL or more, the microbial bacterial agent for antagonizing potato scab can be obtained. Wherein, the fermentation medium is: glucose 15-30g / L, starch 10-15g / L, peptone 15-23g / L, NaCl5-10g / L, KH 2 PO 4 0.1~0.5g / L, the rest is distilled water; pH6.0~8....
Embodiment 3
[0038] Embodiment 3: Strain compound and liquid submerged fermentation
[0039] The various strains are mixed according to the following mass percentages: 30% of Bacillus subtilis fermentation broth, 35% of actinomycetes fermentation broth, and 35% of Streptomyces roseus flavum fermentation broth. Inoculate the mixed bacterial liquid into a fermenter filled with fermentation medium, the inoculum amount is 3% of the total fermentation medium mass, and cultivate at 30°C for 5 days, so that the number of bacteria in the fermentation liquid reaches 10×10 8 individual / mL or more, the microbial bacterial agent for antagonizing potato scab can be obtained. Wherein, the fermentation medium is: glucose 15-30g / L, starch 10-15g / L, peptone 15-23g / L, NaCl5-10g / L, KH 2 PO 4 0.1~0.5g / L, the rest is distilled water; pH6.0~8.0.
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