Gopalamicin derivatives and application of same in inhibition of infection by drug-resistant bacteria and drug-resistant mycobacterium tuberculosis

A kind of technology of Mycobacterium tuberculosis, olive leaf element, is applied in the field of fermenting and preparing described natural compound, and marine microbial resource obtains new compound field, can solve the problem that there is no relevant report etc. yet

Active Publication Date: 2014-03-26
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical structures, preparation methods, anti-drug-resistant bacteria and anti-tuberculosis activities of compounds 1 and 2, and the antibacterial activities of olivine olivine derivatives such as compounds 3-6 against drug-resistant bacteria and Mycobacterium tuberculosis have not been seen so far. There are relevant reports

Method used

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  • Gopalamicin derivatives and application of same in inhibition of infection by drug-resistant bacteria and drug-resistant mycobacterium tuberculosis
  • Gopalamicin derivatives and application of same in inhibition of infection by drug-resistant bacteria and drug-resistant mycobacterium tuberculosis
  • Gopalamicin derivatives and application of same in inhibition of infection by drug-resistant bacteria and drug-resistant mycobacterium tuberculosis

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Experimental program
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Effect test

Embodiment 1

[0045] Identification of Streptomyces sp. 7-145

[0046] a) Source of strain: Streptomyces sp. 7-145 was isolated from marine sediments at a depth of about 40m in Heishijiao Bay (38°49'N, 121°34'E) in the Yellow Sea, Dalian, China in our laboratory.

[0047] b) Strain identification: Streptomyces sp. 7-145 was identified based on the conservation of the 16S rRNA gene sequence among microbial species. The genome of Streptomyces sp. 7-145 was extracted, its 16S rRNA gene was amplified by PCR and sequenced, and submitted to the NCBI GenBank database to obtain the accession number JQ782979, and its sequence is shown in SEQ ID NO.1. The results of the 16S rRNA gene comparison of Streptomyces sp. 7-145 showed that the strain was similar to Streptomyces samsunensis M1463 T The similarity is 99.85%. By the proximity method, the phylogenetic evolutionary tree was constructed ( figure 1 ).

[0048] SEQ ID NO.1

[0049] 1 atgaaccggt ttcggccggg gattagtggc gaacgggtga gtaacacgtg ggca...

Embodiment 2

[0073] Fermentation of Streptomyces sp. 7-145

[0074] Take the activated Streptomyces sp. 7-145 plate, pick about 1cm 2 Inoculate the bacterial lawn into 100mL fermentation medium [recipe: 1.0g starch, 2.5g glucose, 1.0g cottonseed cake powder, 0.3g peptone, inorganic salt such as KH 2 PO 4 0.01g, MgSO 4 0.01g, NaCl0.5g, CaCO 3 0.5g, etc., deionized water 100mL], in a 500mL shake flask, 28 ℃, 200r / min shaker culture 120h.

Embodiment 3

[0075] The extraction of fermented liquid and the obtaining of extract

[0076]Streptomyces sp. 7-145 fermentation broth was separated from solid to liquid to obtain filtrate and mycelium; mycelium was extracted by ultrasonic extraction with acetone to obtain acetone extract of mycelium; the filtrate was adsorbed by Amberlite XAD7HP macroporous adsorption resin column , sequentially eluted with water, 50%, 100% acetone; the 50%, 100% acetone eluate part was combined with the acetone extract of mycelium, concentrated under reduced pressure to remove acetone to obtain an aqueous solution, extracted with ethyl acetate; ethyl acetate The extracted part was concentrated under reduced pressure to obtain the fermented liquid extract.

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Abstract

The invention relates to gopalamicin derivatives and application of the same in inhibition of infection by drug-resistant bacteria and drug-resistant mycobacterium tuberculosis. The derivatives are originated from fermentation products of marine actinomycete Streptomyces sp. 7-145. Results of experimental study show that discovered compounds with novel structures and known structures all have strong antibacterial activity on tested drug-resistant bacteria and drug-resistant mycobacterium tuberculosis and are expected to become clinically-useful novel drugs used for inhibition of infection by drug-resistant bacteria and drug-resistant mycobacterium tuberculosis.

Description

Technical field: [0001] The invention relates to obtaining new compounds from marine microbial resources; the invention relates to a method for fermenting and preparing the natural compounds; the invention also relates to the application of the compounds in resisting multiple drug-resistant bacteria and drug-resistant Mycobacterium tuberculosis infection. Background technique: [0002] At present, bacterial drug resistance is becoming more and more serious, and the development of new antibiotics is imminent. Olivine (English name: Elaiophylin, Azalomycin B, Gopalamicin, or Salbomycin) is a class of sixteen-membered ring macrocyclic dilactone antibiotics with double rotational symmetry, which has antibacterial, antiparasitic, immunosuppressive, and stimulatory properties in rumen animals. Growth and other extensive biological activities [Yan Shuling et al., Microbiology Bulletin 2002,29,103-107]. In recent years, our laboratory has developed a series of biologically active s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C12P19/60A61K31/7048A61P31/04A61P31/06C12R1/465
Inventor 甘茂罗王以光肖春玲谭亿吴春彦周红霞
Owner MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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