Anti-glioma active substance streptodepsipeptide P11B, preparation and applications thereof
A technology of streptomycin and glioma, applied in the field of medicine, can solve the problems of limited curative effect, drug resistance, large toxic and side effects, etc.
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[0029] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments. However, the present invention is not limited to these Examples.
[0030] 1. Isolation and cultivation of Streptomyces parvulus P11-23B
[0031] Take 1 gram of air-dried sea mud and dilute it with seawater to a concentration of 1×10 -6 g / mL sample diluent, take 200 μL of sample diluent and evenly disperse it in a petri dish containing Gause’s agar solid medium, and after culturing at 28°C for 7 days, transfer different colonies separately Transfer to another Petri dish containing Gore's agar solid medium, and continue culturing at 28°C for 7 days. Finally, a well-grown single colony (P11-23B) was inoculated on the slant medium of Gowell's agar for culture, and then stored in a 4°C refrigerator for later use.
[0032] 2. Identification of Streptomyces parvulus P11-23B
[0033] The species of the obtained strain P11-23B was identified by 16S r...
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